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Antibacterial and antibiotic-potentiation activities of some Cameroonian food plants against multi-drug resistant gram-negative bacteria
Antibacterial and antibiotic-potentiation activities of some Cameroonian food plants against multi-drug resistant gram-negative bacteria
- 中国结合医学杂志（英文版） 2014年20卷第7期 页码：546-554
- Affiliations：
Department of Biochemistry, Faculty of Science, University of Dschang, Dschang,Cameroon
- Author bio：
- Funds：
DOI：10.1007/s11655-014-1866-7
中图分类号：纸质出版日期：2014，
网络出版日期：2014-6-28，
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Tankeo, S.B., Lacmata, S.T., Noumedem, J.A.K. et al. Antibacterial and antibiotic-potentiation activities of some Cameroonian food plants against multi-drug resistant gram-negative bacteria., Chin. J. Integr. Med. 20, 546–554 (2014). https://doi.org/10.1007/s11655-014-1866-7
Simplice B. Tankeo, Stephen T. Lacmata, Jaures A. K. Noumedem, et al. Antibacterial and antibiotic-potentiation activities of some Cameroonian food plants against multi-drug resistant gram-negative bacteria[J]. Chinese Journal of Integrative Medicine, 2014,20(7):546-554.
Tankeo, S.B., Lacmata, S.T., Noumedem, J.A.K. et al. Antibacterial and antibiotic-potentiation activities of some Cameroonian food plants against multi-drug resistant gram-negative bacteria., Chin. J. Integr. Med. 20, 546–554 (2014). https://doi.org/10.1007/s11655-014-1866-7 DOI：
Simplice B. Tankeo, Stephen T. Lacmata, Jaures A. K. Noumedem, et al. Antibacterial and antibiotic-potentiation activities of some Cameroonian food plants against multi-drug resistant gram-negative bacteria[J]. Chinese Journal of Integrative Medicine, 2014,20(7):546-554. DOI： 10.1007/s11655-014-1866-7.
摘要
To evaluate the in vitro antibacterial properties and the ability to potentiate some common antibiotics effects of the methanol extracts of 11 Cameroonian food plants on 29 Gram-negative bacteria expressing multidrug resistant (MDR) phenotypes. The antimicrobial activity of the extracts was performed using the broth microdilution method. The phytochemical screening of these extracts was also performed using standard methods. Ocimum basilicum
Gnetum africanum and Eucalyptus robusta extracts possessed an antibacterial activity against all the 29 studied bacteria. The extracts from G. africanum and E. robusta were the most active with the lowest minimal inhibitory concentration of 64 μg/mL on Escherichia coli AG100A for both extracts and also against Klebsiella pneumoniae K24 for G. africanum. When tested in the presence of phenylalanine-arginine β-Naphtylamide (PAβN)
an efflux pump inhibitor
the extract of Thymus vulgaris and E. Robusta showed the best activities on most tested strains. E. Robusta extract showed good synergistic effects
improving the activity of commonly used antibiotics in about 85% of cases. The overall results obtained provide the baseline information for the use of the tested plants in the treatment of bacterial infections.
Abstract
To evaluate the in vitro antibacterial properties and the ability to potentiate some common antibiotics effects of the methanol extracts of 11 Cameroonian food plants on 29 Gram-negative bacteria expressing multidrug resistant (MDR) phenotypes. The antimicrobial activity of the extracts was performed using the broth microdilution method. The phytochemical screening of these extracts was also performed using standard methods. Ocimum basilicum
Gnetum africanum and Eucalyptus robusta extracts possessed an antibacterial activity against all the 29 studied bacteria. The extracts from G. africanum and E. robusta were the most active with the lowest minimal inhibitory concentration of 64 μg/mL on Escherichia coli AG100A for both extracts and also against Klebsiella pneumoniae K24 for G. africanum. When tested in the presence of phenylalanine-arginine β-Naphtylamide (PAβN)
an efflux pump inhibitor
the extract of Thymus vulgaris and E. Robusta showed the best activities on most tested strains. E. Robusta extract showed good synergistic effects
improving the activity of commonly used antibiotics in about 85% of cases. The overall results obtained provide the baseline information for the use of the tested plants in the treatment of bacterial infections.
关键词
food plantsCameroonmultidrug resistant Gram-negative bacteriaefflux pumps
Keywords
food plantsCameroonmultidrug resistant Gram-negative bacteriaefflux pumps
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