Effects of Panax Quinquefolium Saponin on phosphatidylinositol 3-kinase/serine threonine kinase pathway of neonatal rat myocardial cells subjected to hypoxia

Chun-yu Guo; Xiao-juan Ma; Jing-shang Wang; Ying Shi; Xin Liu; Hui-jun Yin; Ke-ji Chen

doi:10.1007/s11655-014-1881-8

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Effects of Panax Quinquefolium Saponin on phosphatidylinositol 3-kinase/serine threonine kinase pathway of neonatal rat myocardial cells subjected to hypoxia

OriginalPaper | Updated：2021-08-27

- Effects of Panax Quinquefolium Saponin on phosphatidylinositol 3-kinase/serine threonine kinase pathway of neonatal rat myocardial cells subjected to hypoxia
- Effects of Panax Quinquefolium Saponin on phosphatidylinositol 3-kinase/serine threonine kinase pathway of neonatal rat myocardial cells subjected to hypoxia
- 中国结合医学杂志（英文版） 2015年21卷第5期页码：384-388
- Affiliations：
  
  Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the China Postdoctoral Science Fondation (No. 20100480427) and the National Natural Science Fondation of China (No. 81041038)
- DOI：10.1007/s11655-014-1881-8
  中图分类号：
- 纸质出版日期：2015，
  
  网络出版日期：2014-7-14，
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Guo, Cy., Ma, Xj., Wang, Js. et al. Effects of Panax Quinquefolium Saponin on phosphatidylinositol 3-kinase/serine threonine kinase pathway of neonatal rat myocardial cells subjected to hypoxia., Chin. J. Integr. Med. 21, 384–388 (2015). https://doi.org/10.1007/s11655-014-1881-8

Chun-yu Guo, Xiao-juan Ma, Jing-shang Wang, et al. Effects of Panax Quinquefolium Saponin on phosphatidylinositol 3-kinase/serine threonine kinase pathway of neonatal rat myocardial cells subjected to hypoxia[J]. Chinese Journal of Integrative Medicine, 2015,21(5):384-388.
Guo, Cy., Ma, Xj., Wang, Js. et al. Effects of Panax Quinquefolium Saponin on phosphatidylinositol 3-kinase/serine threonine kinase pathway of neonatal rat myocardial cells subjected to hypoxia., Chin. J. Integr. Med. 21, 384–388 (2015). https://doi.org/10.1007/s11655-014-1881-8 DOI：

Chun-yu Guo, Xiao-juan Ma, Jing-shang Wang, et al. Effects of Panax Quinquefolium Saponin on phosphatidylinositol 3-kinase/serine threonine kinase pathway of neonatal rat myocardial cells subjected to hypoxia[J]. Chinese Journal of Integrative Medicine, 2015,21(5):384-388. DOI： 10.1007/s11655-014-1881-8.

摘要

To explore the effects of Panax Quinquefolium Saponin (PQS) on phosphatidylinositol 3-kinase/serine threonine kinase (PI3K/Akt) pathway of neonatal rat myocardial cells subjected to hypoxia. Neonatal rat myocardial cells were cultured in vitro. After the myocardial cell injury was induced by hypoxia

the cells were randomized into 5 groups: the normal group

the model group

the positive control group (Ciclosporin A

2 µ mol/L)

the low-dose PQS group (PQSL

25mg/L)

and the high-dose PQS group (PQSH

50 mg/L). Morphology and behavior of myocardial cells were observed under an inverted microscope. Apoptosis rate and lactate dehydrogenase (LDH) leakage rate of myocardial cells were determined by colorimetry. Mitochondrial transmembrane potential was assessed using a fluorexon laser. Phospho-glycogen synthase kinase (GSK)-3β and phospho-Akt as well as cytochrome C were determined by Western blot LDH leakage in the Ciclosporin A group

PQSH group and PQSL group reduced progressively compared with the model group (P<0.05). Akt and GSK-3β was strongly phosphorylated after treatment with Ciclosporin A and PQS compared with the model group (P<0.05

P<0.01). Compared with the model group (16.41±1.74; 35.28±6.30)

both the integrated optical density of mitochondrial permeability transition pore (MPTP) and the mitochondrial transmembrane potential significantly increased in the PQSH group (42.74±2.12; 71.36±6.54) and the PQSL group (39.58±1.49; 66.99±5.45; P<0.05

P<0.01). However

the protein of cytochrome C outside the mitochondrion decreased in the PQSH group (273.66±14.61) and the PQSL group (259.62±17.31) compared with the model group (502.41±17.76; P<0.05). Through activation of the PI3K/Akt pathway and inhibition of the MPTP

PQS might protect the heart against ischemia injury and apoptosis of myocardial cells.

Abstract

the cells were randomized into 5 groups: the normal group

the model group

the positive control group (Ciclosporin A

2 µ mol/L)

the low-dose PQS group (PQSL

25mg/L)

and the high-dose PQS group (PQSH

P<0.01). Compared with the model group (16.41±1.74; 35.28±6.30)

P<0.01). However

PQS might protect the heart against ischemia injury and apoptosis of myocardial cells.

关键词

Panax Quinquefolium Saponinischemic cardiomyocytesphosphatidylinositol 3-kinase/serinethreonine kinasemitochondrial permeability transition pore

Keywords

Panax Quinquefolium Saponinischemic cardiomyocytesphosphatidylinositol 3-kinase/serinethreonine kinasemitochondrial permeability transition pore

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