Neuroprotective effect of Shenfu Injection (参附注射液) following cardiac arrest in pig correlates with improved mitochondrial function and cerebral glucose uptake

Yi Zhang; Chun-sheng Li; Cai-jun Wu; Jun Yang; Chen-chen Hang

doi:10.1007/s11655-014-1890-7

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Neuroprotective effect of Shenfu Injection (参附注射液) following cardiac arrest in pig correlates with improved mitochondrial function and cerebral glucose uptake

OriginalPaper | Updated：2021-08-27

- Neuroprotective effect of Shenfu Injection (参附注射液) following cardiac arrest in pig correlates with improved mitochondrial function and cerebral glucose uptake
- Neuroprotective effect of Shenfu Injection (参附注射液) following cardiac arrest in pig correlates with improved mitochondrial function and cerebral glucose uptake
- 中国结合医学杂志（英文版） 2014年20卷第11期页码：835-843
- Affiliations：
  
  1. Department of Hyperbaric Oxygen, Beijing Chao-Yang Hospital, Capital Medical University,Beijing,China
  2. Department of Emergency, Beijing Chao-Yang Hospital, Capital Medical University,Beijing,China
- Author bio：
- Funds：
  
  Supported by the Beijing Natural Science Foundation (No. 7132092) and Beijing Scientific Research Project for Outstanding Doctoral Thesis Guidance Teacher (No. 20121002501)
- DOI：10.1007/s11655-014-1890-7
  中图分类号：
- 纸质出版日期：2014，
  
  网络出版日期：2014-11-20，
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Zhang, Y., Li, Cs., Wu, Cj. et al. Neuroprotective effect of Shenfu Injection (参附注射液) following cardiac arrest in pig correlates with improved mitochondrial function and cerebral glucose uptake., Chin. J. Integr. Med. 20, 835–843 (2014). https://doi.org/10.1007/s11655-014-1890-7

Yi Zhang, Chun-sheng Li, Cai-jun Wu, et al. Neuroprotective effect of Shenfu Injection (参附注射液) following cardiac arrest in pig correlates with improved mitochondrial function and cerebral glucose uptake[J]. Chinese Journal of Integrative Medicine, 2014,20(11):835-843.
Zhang, Y., Li, Cs., Wu, Cj. et al. Neuroprotective effect of Shenfu Injection (参附注射液) following cardiac arrest in pig correlates with improved mitochondrial function and cerebral glucose uptake., Chin. J. Integr. Med. 20, 835–843 (2014). https://doi.org/10.1007/s11655-014-1890-7 DOI：

Yi Zhang, Chun-sheng Li, Cai-jun Wu, et al. Neuroprotective effect of Shenfu Injection (参附注射液) following cardiac arrest in pig correlates with improved mitochondrial function and cerebral glucose uptake[J]. Chinese Journal of Integrative Medicine, 2014,20(11):835-843. DOI： 10.1007/s11655-014-1890-7.

摘要

To test whether Shenfu Injection (参附注射液

SFI) might attenuate the impact of cerebral energy dysfunction after resuscitation in a pig model of cardiac arrest (CA). Thirty-four Wuzhishan miniature inbred pigs were randomly divided into three groups: the SFI group (n=12)

the saline group (SA group

n=12)

and the sham-operated group (sham group

n=10). Following successful return of spontaneous circulation (ROSC) from 8-min untreated ventricular fibrillation

animals received a continuous infusion of either SFI (0.2 mL/min) or saline for 6 h. Cerebral performance category score was evaluated at 24 and 48 h after ROSC

followed by positron emission tomography and computed tomography scans of cerebral glucose uptake. Surviving pigs were euthanized 48 h after ROSC

and the brains were removed for detecting mitochondrial function. Compared with the SA group

SFI treatment produced a better neurologic outcome 48 h after ROSC (P<0.05). However

there was no significant difference of survival rate between the SA and SFI groups (83.3% vs. 81.8%

P>0.05). After ROSC

the SA group showed a decrease in the maximum standardized uptake value of different regions in the brain tissue

where SFI treatment can ameliorate these decreases (P<0.01 or P<0.05). Improved mitochondrial respiratory properties and higher mitochondrial membrane potential were also found following SFI treatment compared with the SA group at 48 h after ROSC (P<0.05 or P<0.01). SFI treatment after resuscitation has significant neuroprotective effects against disruption of cerebral energy metabolism from CA by improving glucose uptake and by normalizing mitochondrial function.

Abstract

To test whether Shenfu Injection (参附注射液

the saline group (SA group

n=12)

and the sham-operated group (sham group

n=10). Following successful return of spontaneous circulation (ROSC) from 8-min untreated ventricular fibrillation

animals received a continuous infusion of either SFI (0.2 mL/min) or saline for 6 h. Cerebral performance category score was evaluated at 24 and 48 h after ROSC

followed by positron emission tomography and computed tomography scans of cerebral glucose uptake. Surviving pigs were euthanized 48 h after ROSC

and the brains were removed for detecting mitochondrial function. Compared with the SA group

SFI treatment produced a better neurologic outcome 48 h after ROSC (P<0.05). However

there was no significant difference of survival rate between the SA and SFI groups (83.3% vs. 81.8%

P>0.05). After ROSC

the SA group showed a decrease in the maximum standardized uptake value of different regions in the brain tissue

关键词

cardiopulmonary resuscitationShenfu Injectionpositron emission tomography/computed tomographymitochondrial membrane potentialmitochondrial respiratory propertyChinese Medicine

Keywords

cardiopulmonary resuscitationShenfu Injectionpositron emission tomography/computed tomographymitochondrial membrane potentialmitochondrial respiratory propertyChinese Medicine

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