Genome-wide association study on susceptibility genes associated with yang-deficiency constitution: A small sample case-control study

Shi-lin Yao; Qi Wang; Zu-zhi Zhang; Jun-xia Wu; Nan Cheng; Xia Xu; Guang-yan Xie; Jian Cao

doi:10.1007/s11655-014-1957-5

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Genome-wide association study on susceptibility genes associated with yang-deficiency constitution: A small sample case-control study

OriginalPaper | Updated：2021-08-27

- Genome-wide association study on susceptibility genes associated with yang-deficiency constitution: A small sample case-control study
- Genome-wide association study on susceptibility genes associated with yang-deficiency constitution: A small sample case-control study
- 中国结合医学杂志（英文版） 2015年21卷第8期页码：601-609
- Affiliations：
  
  1. Department of Science and Technology, Anhui University of Chinese Medicine,Hefei,China
  2. College of Basic Medicine, Beijing University of Chinese Medicine,Beijing,China
  3. College of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine,Hefei,China
  4. Institute of Neuropathy, Anhui University of Chinese Medicine,Hefei,China
  5. Clinical College of Traditional Chinese Medicine, Anhui University of Chinese Medicine,Hefei,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81072715)
- DOI：10.1007/s11655-014-1957-5
  中图分类号：
- 纸质出版日期：2015，
  
  网络出版日期：2014-12-22，
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Yao, Sl., Wang, Q., Zhang, Zz. et al. Genome-wide association study on susceptibility genes associated with yang-deficiency constitution: A small sample case-control study., Chin. J. Integr. Med. 21, 601–609 (2015). https://doi.org/10.1007/s11655-014-1957-5

Shi-lin Yao, Qi Wang, Zu-zhi Zhang, et al. Genome-wide association study on susceptibility genes associated with yang-deficiency constitution: A small sample case-control study[J]. Chinese Journal of Integrative Medicine, 2015,21(8):601-609.
Yao, Sl., Wang, Q., Zhang, Zz. et al. Genome-wide association study on susceptibility genes associated with yang-deficiency constitution: A small sample case-control study., Chin. J. Integr. Med. 21, 601–609 (2015). https://doi.org/10.1007/s11655-014-1957-5 DOI：

Shi-lin Yao, Qi Wang, Zu-zhi Zhang, et al. Genome-wide association study on susceptibility genes associated with yang-deficiency constitution: A small sample case-control study[J]. Chinese Journal of Integrative Medicine, 2015,21(8):601-609. DOI： 10.1007/s11655-014-1957-5.

摘要

To explore susceptibility genes associated with yang-deficiency constitution using single nucleotide polymorphism (SNP) genotyping. Based on an epidemiological survey

30 volunteers with yang-deficiency constitution and 30 volunteers with a balanced constitution were included according to the Classification and Determination Standards of Constitutions in Traditional Chinese Medicine. Peripheral blood was collected and DNA was extracted from white blood cells. A genome-wide association study (GWAS) was conducted by SNP 6.0 genotyping at the Beijing CapitalBio Corporation Ltd. A minimum association P-value (Fisher’s exact value) of less than 10-4 in the allele

genotype

dominant

and recessive models served as the standard for significant association of SNP with yang-deficiency constitution. Among the four genetic models

a total of 42 SNPs were significantly associated with yang-deficiency constitution (Fisher’s exact P-values P<10-4). These SNPs were adjacent to more than 20 genes

including RGS6

mGluR5

GAPDHL19

and IKZF1. Yang-deficiency constitution exhibits the characteristics of polygenic inheritance. This pilot study suggests that the polymorphisms in RGS6

mGluR5

GAPDHL19

and IKZF1 are associated with changes in cyclic adenosine monophosphate and cyclic guanosine monophosphate levels

memory

metabolic energy status

and immune function

respectively in people with yang-deficiency constitution.

Abstract

To explore susceptibility genes associated with yang-deficiency constitution using single nucleotide polymorphism (SNP) genotyping. Based on an epidemiological survey

genotype

dominant

and recessive models served as the standard for significant association of SNP with yang-deficiency constitution. Among the four genetic models

a total of 42 SNPs were significantly associated with yang-deficiency constitution (Fisher’s exact P-values P<10-4). These SNPs were adjacent to more than 20 genes

including RGS6

mGluR5

GAPDHL19

and IKZF1. Yang-deficiency constitution exhibits the characteristics of polygenic inheritance. This pilot study suggests that the polymorphisms in RGS6

mGluR5

GAPDHL19

and IKZF1 are associated with changes in cyclic adenosine monophosphate and cyclic guanosine monophosphate levels

memory

metabolic energy status

and immune function

respectively in people with yang-deficiency constitution.

关键词

yang-deficiency constitutiongenome-wide association analysissingle nucleotide polymorphismcase-control studyChinese Medicine

Keywords

yang-deficiency constitutiongenome-wide association analysissingle nucleotide polymorphismcase-control studyChinese Medicine

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