Effects of berberine and cinnamic acid on palmitic acid-induced intracellular triglyceride accumulation in NIT-1 pancreatic β cells

Li Zhao; Shu-jun Jiang; Fu-er Lu; Li-jun Xu; Xin Zou; Kai-fu Wang; Hui Dong

doi:10.1007/s11655-014-1986-0

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Effects of berberine and cinnamic acid on palmitic acid-induced intracellular triglyceride accumulation in NIT-1 pancreatic β cells

OriginalPaper | Updated：2021-08-27

- Effects of berberine and cinnamic acid on palmitic acid-induced intracellular triglyceride accumulation in NIT-1 pancreatic β cells
- Effects of berberine and cinnamic acid on palmitic acid-induced intracellular triglyceride accumulation in NIT-1 pancreatic β cells
- 中国结合医学杂志（英文版） 2016年22卷第7期页码：496-502
- Affiliations：
  
  1. Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan,China
  2. Department of Nephrology, Wuhan Integrated Traditional Chinese Medicine and Western Medicine Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81373871 and 81273683)
- DOI：10.1007/s11655-014-1986-0
  中图分类号：
- 纸质出版日期：2016，
  
  网络出版日期：2014-12-9，
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Zhao, L., Jiang, Sj., Lu, Fe. et al. Effects of berberine and cinnamic acid on palmitic acid-induced intracellular triglyceride accumulation in NIT-1 pancreatic β cells., Chin. J. Integr. Med. 22, 496–502 (2016). https://doi.org/10.1007/s11655-014-1986-0

Li Zhao, Shu-jun Jiang, Fu-er Lu, et al. Effects of berberine and cinnamic acid on palmitic acid-induced intracellular triglyceride accumulation in NIT-1 pancreatic β cells[J]. Chinese Journal of Integrative Medicine, 2016,22(7):496-502.
Zhao, L., Jiang, Sj., Lu, Fe. et al. Effects of berberine and cinnamic acid on palmitic acid-induced intracellular triglyceride accumulation in NIT-1 pancreatic β cells., Chin. J. Integr. Med. 22, 496–502 (2016). https://doi.org/10.1007/s11655-014-1986-0 DOI：

Li Zhao, Shu-jun Jiang, Fu-er Lu, et al. Effects of berberine and cinnamic acid on palmitic acid-induced intracellular triglyceride accumulation in NIT-1 pancreatic β cells[J]. Chinese Journal of Integrative Medicine, 2016,22(7):496-502. DOI： 10.1007/s11655-014-1986-0.

摘要

To investigate the effects of berberine (BBR) and cinnamic acid (CA)

the main active components in Jiaotai Pill (交泰丸

JTP)

on palmitic acid (PA)-induced intracellular triglyceride (TG) accumulation in NIT-1 pancreatic β cells. Cells were incubated in culture medium containing PA (0.25 mmol/L) for 24 h. Then treatments with BBR (10 μmol/L)

CA (100 μmol/L) and the combination of BBR and CA (BBR+CA) were performed respectively. Intracellular lipid accumulation was assessed by Oil Red O staining and TG content was measured by colorimetric assay. The expression of adenosine monophosphate-activated protein kinase (AMPK) protein and its downstream lipogenic and fatty acid oxidation genes

including fatty acid synthase (FAS)

acetyl-coA carboxylase (ACC)

phosphorylation acetyl-coA carboxylase (pACC)

carnitine acyl transferase 1 (CPT-1) and sterol regulating element binding protein 1c (SREBP-1c) were determined by Western blot or real time polymerase chain reaction. PA induced an obvious lipid accumulation and a significant increase in intracellular TG content in NIT-1 cells. PA also induced a remarkable decrease in AMPK protein expression and its downstream targets such as pACC and CPT-1. Meanwhile

AMPK downstream lipogenic genes including SREBP-1c mRNA

FAS and ACC protein expressions were increased. Treatments with BBR and BBR+CA

superior to CA

significantly reversed the above genes changes in NIT-1 pancreatic β cells. However

the synergistic effect of BBR and CA on intracellular TG content was not observed in the present study. It can be concluded that in vitro

BBR and BBR+CA could inhibit PA-induced lipid accumulation by decreasing lipogenesis and increasing lipid oxidation in NIT-1 pancreatic β cells.

Abstract

To investigate the effects of berberine (BBR) and cinnamic acid (CA)

the main active components in Jiaotai Pill (交泰丸

JTP)

including fatty acid synthase (FAS)

acetyl-coA carboxylase (ACC)

phosphorylation acetyl-coA carboxylase (pACC)

AMPK downstream lipogenic genes including SREBP-1c mRNA

FAS and ACC protein expressions were increased. Treatments with BBR and BBR+CA

superior to CA

significantly reversed the above genes changes in NIT-1 pancreatic β cells. However

the synergistic effect of BBR and CA on intracellular TG content was not observed in the present study. It can be concluded that in vitro

BBR and BBR+CA could inhibit PA-induced lipid accumulation by decreasing lipogenesis and increasing lipid oxidation in NIT-1 pancreatic β cells.

关键词

Berberinecinnamic acidpalmitic acidNIT-1 cellslipidChinese MedicineJiaotai Pill

Keywords

Berberinecinnamic acidpalmitic acidNIT-1 cellslipidChinese MedicineJiaotai Pill

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