Effects of panaxadiol saponins component as a new Chinese patent medicine on proliferation, differentiation and corresponding gene expression profile of megakaryocytes

Wei-wei Wen; Xin Sun; Hai-feng Zhuang; Xiao-jie Lin; Zhi-yin Zheng; Rui-lan Gao; Li-ming Yin

doi:10.1007/s11655-015-1970-3

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Effects of panaxadiol saponins component as a new Chinese patent medicine on proliferation, differentiation and corresponding gene expression profile of megakaryocytes

OriginalPaper | Updated：2021-08-27

- Effects of panaxadiol saponins component as a new Chinese patent medicine on proliferation, differentiation and corresponding gene expression profile of megakaryocytes
- Effects of panaxadiol saponins component as a new Chinese patent medicine on proliferation, differentiation and corresponding gene expression profile of megakaryocytes
- 中国结合医学杂志（英文版） 2016年22卷第1期页码：28-35
- Affiliations：
  
  1. The Third People’s Hospital of Hangzhou,Hangzhou,China
  2. Department of Oncology, Zhejiang Provincial People’s Hospital,Hangzhou,China
  3. Institution of Hematology Research, the First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou,China
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 81373876), Zhejiang Provincial Natural Science Foundation (No. LY14H290004) and Science and Technology Program of Zhejiang Province (No. 2010C33098)
- DOI：10.1007/s11655-015-1970-3
  中图分类号：
- 纸质出版日期：2016，
  
  网络出版日期：2015-4-24，
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Wen, Ww., Sun, X., Zhuang, Hf. et al. Effects of panaxadiol saponins component as a new Chinese patent medicine on proliferation, differentiation and corresponding gene expression profile of megakaryocytes., Chin. J. Integr. Med. 22, 28–35 (2016). https://doi.org/10.1007/s11655-015-1970-3

Wei-wei Wen, Xin Sun, Hai-feng Zhuang, et al. Effects of panaxadiol saponins component as a new Chinese patent medicine on proliferation, differentiation and corresponding gene expression profile of megakaryocytes[J]. Chinese Journal of Integrative Medicine, 2016,22(1):28-35.
Wen, Ww., Sun, X., Zhuang, Hf. et al. Effects of panaxadiol saponins component as a new Chinese patent medicine on proliferation, differentiation and corresponding gene expression profile of megakaryocytes., Chin. J. Integr. Med. 22, 28–35 (2016). https://doi.org/10.1007/s11655-015-1970-3 DOI：

Wei-wei Wen, Xin Sun, Hai-feng Zhuang, et al. Effects of panaxadiol saponins component as a new Chinese patent medicine on proliferation, differentiation and corresponding gene expression profile of megakaryocytes[J]. Chinese Journal of Integrative Medicine, 2016,22(1):28-35. DOI： 10.1007/s11655-015-1970-3.

摘要

To investigate the effects of panaxadiol saponins component (PDS-C) isolated from total saponins of panax ginseng on proliferation

differentiation and corresponding gene expression profile of megakaryocytes. Bone marrow culture of colony forming assay of megakaryocytic progenitor cells (CFU-MK) was observed for the promoting proliferation mediated by PDS-C

and differentiation of megakaryocytic blasts caused by PDS-C was analyzed with flow cytometry in CHRF-288 and Meg-01 cells

as well as proliferation

differentiation-related genes expression profile and protein expression levels were detected by human gene expression microarray and western blot. In response to PDS-C 10

20 and 50 mg/L

CFU-MK from 10 human bone marrow samples was increased by 28.9%±2.7%

41.0%±3.2% and 40.5%±2.6% over untreated control

respectively (P <0.01

each). Flow cytometry analysis showed that PDS-C treated CHRF-288 cells and Meg-01 cells significantly increased in CD42b

CD41

TSP and CD36 positive ratio

respectively. PDS-C induced 29 genes up-regulated more than two-fold commonly in both cells detected by human expression microarray representing 4000 known genes. The protein expression levels of ZNF91

c-Fos

BTF3a

GATA-1

RGS2

NDRG2 and RUNX1 were increased with western blot in correspond to microarray results. PDS-C as an effective component for hematopoiesis

play the role to enhance proliferation and differentiation of megakaryocytes

also up-regulated expression of proliferation

differentiation-related genes and proteins in vitro.

Abstract

To investigate the effects of panaxadiol saponins component (PDS-C) isolated from total saponins of panax ginseng on proliferation

and differentiation of megakaryocytic blasts caused by PDS-C was analyzed with flow cytometry in CHRF-288 and Meg-01 cells

as well as proliferation

differentiation-related genes expression profile and protein expression levels were detected by human gene expression microarray and western blot. In response to PDS-C 10

20 and 50 mg/L

CFU-MK from 10 human bone marrow samples was increased by 28.9%±2.7%

41.0%±3.2% and 40.5%±2.6% over untreated control

respectively (P <0.01

each). Flow cytometry analysis showed that PDS-C treated CHRF-288 cells and Meg-01 cells significantly increased in CD42b

CD41

TSP and CD36 positive ratio

c-Fos

BTF3a

GATA-1

RGS2

NDRG2 and RUNX1 were increased with western blot in correspond to microarray results. PDS-C as an effective component for hematopoiesis

play the role to enhance proliferation and differentiation of megakaryocytes

also up-regulated expression of proliferation

differentiation-related genes and proteins in vitro.

关键词

panaxadiol saponinsmegakaryocytegene expression profileproliferationdifferentiation

Keywords

panaxadiol saponinsmegakaryocytegene expression profileproliferationdifferentiation

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