Neuroprotective effects of electroacupuncture on hypoxic-ischemic encephalopathy in newborn rats are associated with increased expression of GDNF-RET and protein kinase B

Tao Xu; Neng-gui Xu; Zhong-hua Yang; Yan-zhen Wan; Qing-long Wu; Kang-bai Huang

doi:10.1007/s11655-015-1972-1

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Neuroprotective effects of electroacupuncture on hypoxic-ischemic encephalopathy in newborn rats are associated with increased expression of GDNF-RET and protein kinase B

OriginalPaper | Updated：2021-08-27

- Neuroprotective effects of electroacupuncture on hypoxic-ischemic encephalopathy in newborn rats are associated with increased expression of GDNF-RET and protein kinase B
- Neuroprotective effects of electroacupuncture on hypoxic-ischemic encephalopathy in newborn rats are associated with increased expression of GDNF-RET and protein kinase B
- 中国结合医学杂志（英文版） 2016年22卷第6期页码：457-466
- Affiliations：
  
  1. Traditional Chinese Medicine Department, Qingdao Women and Children Hospital,Shandong Province,Qingdao,China
  2. College of Acupuncture and Moxibustion and Massage, Guangzhou University of Traditional Chinese Medicine,Guangzhou,China
- Author bio：
- Funds：
  
  Supported by the 45th China Postdoctoral Science Foundation (No. 20090450856)
- DOI：10.1007/s11655-015-1972-1
  中图分类号：
- 纸质出版日期：2016，
  
  网络出版日期：2015-5-12，
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Xu, T., Xu, Ng., Yang, Zh. et al. Neuroprotective effects of electroacupuncture on hypoxic-ischemic encephalopathy in newborn rats are associated with increased expression of GDNF-RET and protein kinase B., Chin. J. Integr. Med. 22, 457–466 (2016). https://doi.org/10.1007/s11655-015-1972-1

Tao Xu, Neng-gui Xu, Zhong-hua Yang, et al. Neuroprotective effects of electroacupuncture on hypoxic-ischemic encephalopathy in newborn rats are associated with increased expression of GDNF-RET and protein kinase B[J]. Chinese Journal of Integrative Medicine, 2016,22(6):457-466.
Xu, T., Xu, Ng., Yang, Zh. et al. Neuroprotective effects of electroacupuncture on hypoxic-ischemic encephalopathy in newborn rats are associated with increased expression of GDNF-RET and protein kinase B., Chin. J. Integr. Med. 22, 457–466 (2016). https://doi.org/10.1007/s11655-015-1972-1 DOI：

Tao Xu, Neng-gui Xu, Zhong-hua Yang, et al. Neuroprotective effects of electroacupuncture on hypoxic-ischemic encephalopathy in newborn rats are associated with increased expression of GDNF-RET and protein kinase B[J]. Chinese Journal of Integrative Medicine, 2016,22(6):457-466. DOI： 10.1007/s11655-015-1972-1.

摘要

To explore the neuroprotective effects of electroacupuncture (EA) on hypoxic-ischemic encephalopathy (HIE) and to further investigate the role of glial cell line-derived neurotrophic factor (GDNF) family receptor member RET (rearranged during transfection) and its key downstream phosphatidylinositol 3 kinase (PI-3K)/protein kinase B (Akt) pathway in the process. A total of 220 seven-day-old SD rats (of either sex

from 22 broods) were randomly divided into two groups

one (30 rats) for sham-surgery group and the other (190 rats) for HIE model group. The HIE model was established using the left common carotid artery ligation method in combination with hypoxic treatment. The successfully established rats were randomly divided into five groups

including control model group

EA group

sham-EA group

antagonist group and antagonist plus electroacupuncture group

with 35 rats in each group. Baihui (GV 20)

Dazhui (GV 14)

Quchi (LI 11) and Yongquan (KI 1) acupoints were chosen for acupuncture. EA was performed at Baihui and Quchi for 10 min once a day for continuous 1

7 and 21 days

respectively. The rats were then killed after the operation and injured cerebral cortex was taken for the measurement of neurologic damage by hematoxylin-eosin (HE) staining and the degenerative changes of cortical ultrastructure by transmission electron microscopy. RET mRNA level and Akt protein level were detected by real-time reverse-transcription polymerase chain reaction (RT-PCR) and western blot analysis

respectively. EA could ameliorate neurologic damage of the first somatic sensory area (S1Tr) and alleviate the degenerative changes of ultrastructure of cortical neurons in rats subjected to HIE. And the longer acupuncture treatment lasted

the better its therapeutic effect would be. This was accompanied by gradually increased expression of GDNF family receptor RET at the mRNA level and its downstream signaling Akt at the protein level in the ischemic cortex. EA has neuroprotective effects on HIE and could be a potential therapeutic strategy for HIE in the neonate. Activation of RET/Akt signaling pathway might be involved in this process.

Abstract

from 22 broods) were randomly divided into two groups

including control model group

EA group

sham-EA group

antagonist group and antagonist plus electroacupuncture group

with 35 rats in each group. Baihui (GV 20)

Dazhui (GV 14)

Quchi (LI 11) and Yongquan (KI 1) acupoints were chosen for acupuncture. EA was performed at Baihui and Quchi for 10 min once a day for continuous 1

7 and 21 days

关键词

hypoxic-ischemic encephalopathyelectroacupunctureglial cell line-derived neurotrophic factorrearranged during transfectionprotein kinase B

Keywords

hypoxic-ischemic encephalopathyelectroacupunctureglial cell line-derived neurotrophic factorrearranged during transfectionprotein kinase B

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