Effect of Puerarin on the Pharmacokinetics of Baicalin in Gegen Qinlian Decoction (葛根芩连汤) in Mice

Hui Kong; Xue-qian Wang; Qing-guo Wang; Yan Zhao; Ye Sun; Yue Zhang; Jie-kun Xu; Hui-hua Qu

doi:10.1007/s11655-015-1973-0

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Effect of Puerarin on the Pharmacokinetics of Baicalin in Gegen Qinlian Decoction (葛根芩连汤) in Mice

OriginalPaper | Updated：2021-08-27

- Effect of Puerarin on the Pharmacokinetics of Baicalin in Gegen Qinlian Decoction (葛根芩连汤) in Mice
- Effect of Puerarin on the Pharmacokinetics of Baicalin in Gegen Qinlian Decoction (葛根芩连汤) in Mice
- 中国结合医学杂志（英文版） 2018年24卷第7期页码：525-530
- Affiliations：
  
  1. School of Preclinical Medicine, Beijing University of Chinese Medicine,Beijing,China
  2. Center of Scientific Experiment, Beijing University of Chinese Medicine,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No.81274043)
- DOI：10.1007/s11655-015-1973-0
  中图分类号：
- 纸质出版日期：2018，
  
  网络出版日期：2015-4-24，
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Kong, H., Wang, Xq., Wang, Qg. et al. Effect of Puerarin on the Pharmacokinetics of Baicalin in Gegen Qinlian Decoction (葛根芩连汤) in Mice., Chin. J. Integr. Med. 24, 525–530 (2018). https://doi.org/10.1007/s11655-015-1973-0

Hui Kong, Xue-qian Wang, Qing-guo Wang, et al. Effect of Puerarin on the Pharmacokinetics of Baicalin in Gegen Qinlian Decoction (葛根芩连汤) in Mice[J]. Chinese Journal of Integrative Medicine, 2018,24(7):525-530.
Kong, H., Wang, Xq., Wang, Qg. et al. Effect of Puerarin on the Pharmacokinetics of Baicalin in Gegen Qinlian Decoction (葛根芩连汤) in Mice., Chin. J. Integr. Med. 24, 525–530 (2018). https://doi.org/10.1007/s11655-015-1973-0 DOI：

Hui Kong, Xue-qian Wang, Qing-guo Wang, et al. Effect of Puerarin on the Pharmacokinetics of Baicalin in Gegen Qinlian Decoction (葛根芩连汤) in Mice[J]. Chinese Journal of Integrative Medicine, 2018,24(7):525-530. DOI： 10.1007/s11655-015-1973-0.

摘要

To study the pharmacokinetics of puerarin (PUE) in Gegen Qinlian Decoction (葛根芩连汤

GQD)

and the effects of PUE dosage variations on the pharmacokinetics of baicalin (BAL) in mice. GQD is composed of the concentrated granules of four Chinese herbs. Three dosages with different levels of PUE

including GQD

GQD co-administered with PUE

and GQD co-administration with two times the amount of PUE

were used to research the pharmacokinetics of PUE and BAL in mice. The indirect competitive enzyme-linked immunosorbent assay (icELISA) methods based on an anti PUE-monoclonal antibody (MAb)and BAL-MAb were employed to determine the concentration of PUE and BAL in mice blood. After the co-administration of GQD with PUE

the area under the curves (AUC0–14h) of PUE increased 2.8 times compared with GQD. At the dose of GQD co-administration at two times that of PUE

the (AUC0–14h) of PUE was almost equal to that of GQD co-administration of PUE

showing non-linear pharmacokinetics. The (AUC0–48h) of BAL showed a good dose-related increase of PUE (r=0.993) in the range from 100 to 300 mg/kg

indicating that PUE dramatically affects the absorption of BAL in mice. There was no significant difference in the other pharmacokinetic parameters

such as the first time of maximum concentration (Tmax)

the second Tmax

or the mean residence time. The icELISA methods were successfully applied to pharmacokinetic studies of PUE and BAL in GQD in mice. The dosage variability of PUE of the main ingredient in GQD affects its own pharmacokinetic characteristics and the absorption characteristics of BAL.

Abstract

To study the pharmacokinetics of puerarin (PUE) in Gegen Qinlian Decoction (葛根芩连汤

GQD)

including GQD

GQD co-administered with PUE

and GQD co-administration with two times the amount of PUE

the area under the curves (AUC0–14h) of PUE increased 2.8 times compared with GQD. At the dose of GQD co-administration at two times that of PUE

the (AUC0–14h) of PUE was almost equal to that of GQD co-administration of PUE

showing non-linear pharmacokinetics. The (AUC0–48h) of BAL showed a good dose-related increase of PUE (r=0.993) in the range from 100 to 300 mg/kg

indicating that PUE dramatically affects the absorption of BAL in mice. There was no significant difference in the other pharmacokinetic parameters

such as the first time of maximum concentration (Tmax)

the second Tmax

关键词

pharmacokineticsGegen Qinlian Decoctionindirect competitive enzyme-linked immunosorbent assayPuerarinBaicalinChinese Medicine

Keywords

pharmacokineticsGegen Qinlian Decoctionindirect competitive enzyme-linked immunosorbent assayPuerarinBaicalinChinese Medicine

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