Matrine and CYC116 synergistically inhibit growth and induce apoptosis in multiple myeloma cells

Yu-hong Zhou; Jin-yi Feng; Liang-shun You; Hai-tao Meng; Wen-bin Qian

doi:10.1007/s11655-015-1975-y

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Matrine and CYC116 synergistically inhibit growth and induce apoptosis in multiple myeloma cells

OriginalPaper | Updated：2021-08-27

- Matrine and CYC116 synergistically inhibit growth and induce apoptosis in multiple myeloma cells
- Matrine and CYC116 synergistically inhibit growth and induce apoptosis in multiple myeloma cells
- 中国结合医学杂志（英文版） 2015年21卷第8期页码：635-639
- Affiliations：
  
  1. The First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou,China
  2. Institute of Hematology, the First Affiliated Hospital, College of Medicine, Zhejiang University,Hangzhou,China
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 30871099), Zhejiang Provincial Natural Science Foundation of China (No. R2090392), and Administration of Traditional Chinese Medicine of Zhejiang Province (No. 2000C25)
- DOI：10.1007/s11655-015-1975-y
  中图分类号：
- 纸质出版日期：2015，
  
  网络出版日期：2015-3-24，
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Zhou, Yh., Feng, Jy., You, Ls. et al. Matrine and CYC116 synergistically inhibit growth and induce apoptosis in multiple myeloma cells., Chin. J. Integr. Med. 21, 635–639 (2015). https://doi.org/10.1007/s11655-015-1975-y

Yu-hong Zhou, Jin-yi Feng, Liang-shun You, et al. Matrine and CYC116 synergistically inhibit growth and induce apoptosis in multiple myeloma cells[J]. Chinese Journal of Integrative Medicine, 2015,21(8):635-639.
Zhou, Yh., Feng, Jy., You, Ls. et al. Matrine and CYC116 synergistically inhibit growth and induce apoptosis in multiple myeloma cells., Chin. J. Integr. Med. 21, 635–639 (2015). https://doi.org/10.1007/s11655-015-1975-y DOI：

Yu-hong Zhou, Jin-yi Feng, Liang-shun You, et al. Matrine and CYC116 synergistically inhibit growth and induce apoptosis in multiple myeloma cells[J]. Chinese Journal of Integrative Medicine, 2015,21(8):635-639. DOI： 10.1007/s11655-015-1975-y.

摘要

To investigate whether CYC116 can potentiate matrine-dependent growth inhibition and apoptosis in multiple myeloma (MM) cells. The dose response relationship of matrine to dexamethasone-resistant and dexamethasone-sensitive MM cells was first established. Myeloma RPMI8226 cells were treated with matrine alone or combined with CYC116 for 24 h. Cell proliferation was measured using an MTT assay and apoptosis induction was evaluated by flow cytometry. Activation of the caspase pathway and expression of apoptosis regulator proteins were detected by Western blotting. Matrine significantly induced growth arrest and apoptosis in both drug-resistant and drug-sensitive MM cells. Treatment with the combination of matrine and CYC116 had a stronger cytotoxic effect on MM cells than did single drug treatments. Enhanced apoptosis observed following the combined treatment of matrine and CYC116 was associated with higher levels of activation of caspase-9

caspase-3

and poly adenosine diphosphate ribose polymerase (PARP) and down-regulation of the anti-apoptotic proteins Bcl-2 and Mcl-1 and the signaling proteins p-Akt and nuclear factor κB (NF-κB). CYC116 enhances the growth inhibitory and apoptotic effects of matrine on MM cells.

Abstract

caspase-3

关键词

apoptosisaurora kinaseCYC116inhibitorMatrinemultiple myeloma

Keywords

apoptosisaurora kinaseCYC116inhibitorMatrinemultiple myeloma

references

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