Hydroxysafflor yellow A attenuate lipopolysaccharide-induced endothelium inflammatory injury

Ming Jin; Chun-yan Sun; Bao-xia Zang

doi:10.1007/s11655-015-1976-x

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Hydroxysafflor yellow A attenuate lipopolysaccharide-induced endothelium inflammatory injury

OriginalPaper | Updated：2021-08-27

- Hydroxysafflor yellow A attenuate lipopolysaccharide-induced endothelium inflammatory injury
- Hydroxysafflor yellow A attenuate lipopolysaccharide-induced endothelium inflammatory injury
- 中国结合医学杂志（英文版） 2016年22卷第1期页码：36-41
- Affiliations：
  
  Department of Pharmacology, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing Anzhen Hospital, Capital Medical University,Beijing,China
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 81270103) and Natural Science Foundation of Beijing (No. 7102025)
- DOI：10.1007/s11655-015-1976-x
  中图分类号：
- 纸质出版日期：2016，
  
  网络出版日期：2015-5-27，
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Jin, M., Sun, Cy. & Zang, Bx. Hydroxysafflor yellow A attenuate lipopolysaccharide-induced endothelium inflammatory injury., Chin. J. Integr. Med. 22, 36–41 (2016). https://doi.org/10.1007/s11655-015-1976-x

Ming Jin, Chun-yan Sun, Bao-xia Zang. Hydroxysafflor yellow A attenuate lipopolysaccharide-induced endothelium inflammatory injury[J]. Chinese Journal of Integrative Medicine, 2016,22(1):36-41.
Jin, M., Sun, Cy. & Zang, Bx. Hydroxysafflor yellow A attenuate lipopolysaccharide-induced endothelium inflammatory injury., Chin. J. Integr. Med. 22, 36–41 (2016). https://doi.org/10.1007/s11655-015-1976-x DOI：

Ming Jin, Chun-yan Sun, Bao-xia Zang. Hydroxysafflor yellow A attenuate lipopolysaccharide-induced endothelium inflammatory injury[J]. Chinese Journal of Integrative Medicine, 2016,22(1):36-41. DOI： 10.1007/s11655-015-1976-x.

摘要

This study observed attenuating effect of hydroxysafflor yellow A (HSYA)

an effective ingredient of aqueous extract of Carthamus tinctorius L

on lipopolysaccharide (LPS)-induced endothelium inflammatory injury. Eahy926 human endothelium cell (EC) line was used; thiazolyl blue tetrazolium bromide (MTT) test was assayed to observe the viability of EC; Luciferase reporter gene assay was applied to measure nuclear factor-κB (NF-κB) p65 subunit nuclear binding activity in EC; Western blot technology was used to monitor mitogen activated protein kinase (MAPKs) and NF-κB activation. Reverse transcription polymerase chain reaction (RT-PCR) method was applied to observe intercellular cell adhesion molecule-1 (ICAM-1) and E-selectin mRNA level; EC surface ICAM-1 expression was measured with flow cytometry and leukocyte adhesion to EC was assayed with Rose Bengal spectrophotometry technology. HSYA protected EC viability against LPS-induced injury (P <0.05). LPS-induced NF-κB p65 subunit DNA binding (P <0.01) and nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor α (IκBα) phosphorylation was inhibited by HSYA. HSYA attenuated LPS triggered ICAM-1 and E-selectin mRNA levels elevation and phosphorylation of p38 MAPK or c-Jun N-terminal kinase MAPK. HSYA also inhibited LPS-induced cell surface ICAM-1 protein expression P <0.01) and leukocyte adhesion to EC (P <0.05). HSYA is effective to protect LPS-induced high expression of endothelium adhesive molecule and inflammatory signal transduction.

Abstract

This study observed attenuating effect of hydroxysafflor yellow A (HSYA)

an effective ingredient of aqueous extract of Carthamus tinctorius L

关键词

hydroxysafflor yellow Alipopolysaccharideendothelium cellinflammationadhesive molecule

Keywords

hydroxysafflor yellow Alipopolysaccharideendothelium cellinflammationadhesive molecule

references

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Jin M, Sun CY, Pei CQ, Wang L, Zhang PC. Study of the effect of safflor yellow injection on inhibiting lipopolysaccharide-induced pulmonary inflammatory injury in mice. Chin J Integr Med 2013;19:836–843.

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