Extract of Zuojin Pill (左金丸) induces apoptosis of SGC-7901 cells via mitochondria-dependent pathway

Qiu-xian Peng; Hong-bing Cai; Jiang-li Peng; Kin Lam Yung; Jue Shi; Zhi-xian Mo

doi:10.1007/s11655-015-2043-3

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Extract of Zuojin Pill (左金丸) induces apoptosis of SGC-7901 cells via mitochondria-dependent pathway

OriginalPaper | Updated：2021-08-27

- Extract of Zuojin Pill (左金丸) induces apoptosis of SGC-7901 cells via mitochondria-dependent pathway
- Extract of Zuojin Pill (左金丸) induces apoptosis of SGC-7901 cells via mitochondria-dependent pathway
- 中国结合医学杂志（英文版） 2015年21卷第11期页码：837-845
- Affiliations：
  
  1. School of Chinese Medicine, Southern Medical University,Guangzhou,China
  2. Faculty of Science, Hong Kong Baptist University,Hong Kong SAR,China
  3. College of Pharmacy, Hunan University of Chinese Medicine,Changsha,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-015-2043-3
  中图分类号：
- 纸质出版日期：2015，
  
  网络出版日期：2015-4-6，
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Peng, Qx., Cai, Hb., Peng, Jl. et al. Extract of Zuojin Pill (左金丸) induces apoptosis of SGC-7901 cells via mitochondria-dependent pathway., Chin. J. Integr. Med. 21, 837–845 (2015). https://doi.org/10.1007/s11655-015-2043-3

Qiu-xian Peng, Hong-bing Cai, Jiang-li Peng, et al. Extract of Zuojin Pill (左金丸) induces apoptosis of SGC-7901 cells via mitochondria-dependent pathway[J]. Chinese Journal of Integrative Medicine, 2015,21(11):837-845.
Peng, Qx., Cai, Hb., Peng, Jl. et al. Extract of Zuojin Pill (左金丸) induces apoptosis of SGC-7901 cells via mitochondria-dependent pathway., Chin. J. Integr. Med. 21, 837–845 (2015). https://doi.org/10.1007/s11655-015-2043-3 DOI：

Qiu-xian Peng, Hong-bing Cai, Jiang-li Peng, et al. Extract of Zuojin Pill (左金丸) induces apoptosis of SGC-7901 cells via mitochondria-dependent pathway[J]. Chinese Journal of Integrative Medicine, 2015,21(11):837-845. DOI： 10.1007/s11655-015-2043-3.

摘要

To observe the effects of water extract of Zuojin Pill (左金丸

ZJP) on inhibiting the growth of human gastric cancer cell line SGC-7901 and its potential mechanism. Effects of ZJP on SGC-7901 cells growth were determined by 3-(4

5-dimethyl-2-thiazolyl)-2

5-diphenyl-2-H-tetrazolium bromide (MTT) assay

cell apoptosis and cell cycle were determined by flow cytometry

and apoptosis induction was detected by means of DNA gel electrophoresis. The cellular mechanism of drug-induced cell death was unraveled by assaying oxidative injury level of SGC-7901 cell

mitochondrial membrane potentials

expression of apoptosis-related genes

such as B cell lymphoma/lewkmia-2 (Bcl-2)

Bcl-2 associated X protein (Bax) and cleaved caspase-3 and caspase-9. ZJP exerted evident inhibitory effect on SGC-7901 cells by activating production of reactive oxygen species and elevating Bax/Bcl-2 ratio in SGC-7901 cells

leading to attenuation of mitochondrial membrane potential and DNA fragmentation. ZJP inhibits the cancer cell growth via activating mitochondria-dependent apoptosis pathway. ZJP can potentially serve as an antitumor agent.

Abstract

To observe the effects of water extract of Zuojin Pill (左金丸

ZJP) on inhibiting the growth of human gastric cancer cell line SGC-7901 and its potential mechanism. Effects of ZJP on SGC-7901 cells growth were determined by 3-(4

5-dimethyl-2-thiazolyl)-2

5-diphenyl-2-H-tetrazolium bromide (MTT) assay

cell apoptosis and cell cycle were determined by flow cytometry

and apoptosis induction was detected by means of DNA gel electrophoresis. The cellular mechanism of drug-induced cell death was unraveled by assaying oxidative injury level of SGC-7901 cell

mitochondrial membrane potentials

expression of apoptosis-related genes

such as B cell lymphoma/lewkmia-2 (Bcl-2)

关键词

Zuojin Pillmitochondria-dependent pathwayapoptosisreactive oxygen speciesChinese Medicine

Keywords

Zuojin Pillmitochondria-dependent pathwayapoptosisreactive oxygen speciesChinese Medicine

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