Electroacupuncture attenuates spinal nerve ligation-induced microglial activation mediated by p38 mitogen-activated protein kinase

Yi Liang; Jun-ying Du; Yu-jie Qiu; Jun-fan Fang; Jin Liu; Jian-qiao Fang

doi:10.1007/s11655-015-2045-1

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Electroacupuncture attenuates spinal nerve ligation-induced microglial activation mediated by p38 mitogen-activated protein kinase

OriginalPaper | Updated：2021-08-27

- Electroacupuncture attenuates spinal nerve ligation-induced microglial activation mediated by p38 mitogen-activated protein kinase
- Electroacupuncture attenuates spinal nerve ligation-induced microglial activation mediated by p38 mitogen-activated protein kinase
- 中国结合医学杂志（英文版） 2016年22卷第9期页码：704-713
- Affiliations：
  
  1. Department of Neurobiology and Acupuncture Research, The Third Clinical Medical College, Zhejiang Chinese Medical University,Hangzhou,China
  2. Department of Acupuncture and Moxibustion, The Third Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou,China
  3. Department of Acupuncture and Moxibustion, Xixi Hospital of Hangzhou,Hangzhou,China
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 81102643), Zhejiang Provincial Natural Science Foundation (No. Y2091151), State Administration of Traditional Chinese Medicine (Acupuncture) of Key Subjects Construction Funding (No. [2009]30), and Research Fund of Zhejiang Provincial First-Foremost Key Subject—Acupuncture and Moxibustion (No. [2008]255)
- DOI：10.1007/s11655-015-2045-1
  中图分类号：
- 纸质出版日期：2016，
  
  网络出版日期：2015-4-6，
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Liang, Y., Du, Jy., Qiu, Yj. et al. Electroacupuncture attenuates spinal nerve ligation-induced microglial activation mediated by p38 mitogen-activated protein kinase., Chin. J. Integr. Med. 22, 704–713 (2016). https://doi.org/10.1007/s11655-015-2045-1

Yi Liang, Jun-ying Du, Yu-jie Qiu, et al. Electroacupuncture attenuates spinal nerve ligation-induced microglial activation mediated by p38 mitogen-activated protein kinase[J]. Chinese Journal of Integrative Medicine, 2016,22(9):704-713.
Liang, Y., Du, Jy., Qiu, Yj. et al. Electroacupuncture attenuates spinal nerve ligation-induced microglial activation mediated by p38 mitogen-activated protein kinase., Chin. J. Integr. Med. 22, 704–713 (2016). https://doi.org/10.1007/s11655-015-2045-1 DOI：

Yi Liang, Jun-ying Du, Yu-jie Qiu, et al. Electroacupuncture attenuates spinal nerve ligation-induced microglial activation mediated by p38 mitogen-activated protein kinase[J]. Chinese Journal of Integrative Medicine, 2016,22(9):704-713. DOI： 10.1007/s11655-015-2045-1.

摘要

To investigate whether analgesic effect of electroacupuncture (EA) is affected by p38 mitogen-activated protein kinase (p38 MAPK) on microglia. There were two experiments. The experiment 1: 40 male Sprague-Dawley (SD) rats were randomly divided into the normal

surgery

EA and sham EA groups

and the L5 spinal nerve ligation (SNL) on the right side was used to establish neuropathic pain model. EA was applied to bilateral Zusanli (ST36) and Kunlun (BL60) at 24

48 and 72 h after SNL for 30 min

once per day. The paw withdrawal thresholds (PWTs) were measured before surgery (as base) and at 24

49 and 73 h after surgery. Phospho-p38 MAPK (p-p38 MAPK)

oxycocin-42 (OX-42

marker of microglia)

and glial fibrillary acidic protein (GFAP

marker of astrocyte) in bilateral spinal cord dorsal horn (SCDH) were detected by immunofluorescence

respectively. The experiment 2: 40 male SD rats were cannulated for SNL-induced neuropathic pain

and then were randomly divided into the dimethyl sulfoxide (DMSO)

EA plus DMSO

4-(4-fluorophenyl)-2-(4-methylsulfonylpheny)-5-(4-pyridyl)-1H-imidazole (SB203580) and EA plus SB203580 groups. SB203580 (30 nmol/L) was administered 5 min prior to EA treatment. The PWTs and OX-42 in bilateral SCDH were measured as mentioned above. SNL-induced neuropathic pain reduced PWTs and increased the expression of p-p38 MAPK and OX-42 in bilateral lumbar SCDH of rats (P<0.01). Spinal p-p38 MAPK was only co-localized with OX-42 in our study. EA treatment significantly alleviated SNL-mediated mechanical hyperalgesia

and suppressed the expression of p-p38 MAPK and OX-42 in lumbar SCDH (P<0.05 or P<0.01). Intrathecal injection of low dose SB203580 had no influence on PWTs (P>0.05)

but significantly inhibited the expression of OX-42 positive cells in bilateral SCDH (P<0.01 or P<0.05). EA plus SB203580 synergistically increased PWTs

and reduced the expression of bilateral spinal OX-42 (P<0.01 or P<0.05). The central mechanism of EA-induced anti-hyperalgesia may be partially associated with the reduced expression of p-p38 MAPK

and subsequently reducing the activation of OX-42 in neuropathic pain. Therefore

EA may be a new complementary and alternative therapy for neuropathic pain.

Abstract

surgery

EA and sham EA groups

and the L5 spinal nerve ligation (SNL) on the right side was used to establish neuropathic pain model. EA was applied to bilateral Zusanli (ST36) and Kunlun (BL60) at 24

48 and 72 h after SNL for 30 min

once per day. The paw withdrawal thresholds (PWTs) were measured before surgery (as base) and at 24

49 and 73 h after surgery. Phospho-p38 MAPK (p-p38 MAPK)

oxycocin-42 (OX-42

marker of microglia)

and glial fibrillary acidic protein (GFAP

marker of astrocyte) in bilateral spinal cord dorsal horn (SCDH) were detected by immunofluorescence

respectively. The experiment 2: 40 male SD rats were cannulated for SNL-induced neuropathic pain

and then were randomly divided into the dimethyl sulfoxide (DMSO)

EA plus DMSO

and suppressed the expression of p-p38 MAPK and OX-42 in lumbar SCDH (P<0.05 or P<0.01). Intrathecal injection of low dose SB203580 had no influence on PWTs (P>0.05)

but significantly inhibited the expression of OX-42 positive cells in bilateral SCDH (P<0.01 or P<0.05). EA plus SB203580 synergistically increased PWTs

and reduced the expression of bilateral spinal OX-42 (P<0.01 or P<0.05). The central mechanism of EA-induced anti-hyperalgesia may be partially associated with the reduced expression of p-p38 MAPK

and subsequently reducing the activation of OX-42 in neuropathic pain. Therefore

EA may be a new complementary and alternative therapy for neuropathic pain.

关键词

electroacupunctureneuropathic painmicrogliap38 mitogen-activated protein kinaseoxycocin-42

Keywords

electroacupunctureneuropathic painmicrogliap38 mitogen-activated protein kinaseoxycocin-42

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