Compound Tufuling Granules (复方土茯苓颗粒) regulate glucose transporter 9 expression in kidney to influence serum uric acid level in hyperuricemia mice

Ying-wan Liu; Wei-feng Sun; Xian-xian Zhang; Jing Li; Huan-huan Zhang

doi:10.1007/s11655-015-2052-2

Your Location：

Home >

Browse articles >

Compound Tufuling Granules (复方土茯苓颗粒) regulate glucose transporter 9 expression in kidney to influence serum uric acid level in hyperuricemia mice

OriginalPaper | Updated：2021-08-27

- Compound Tufuling Granules (复方土茯苓颗粒) regulate glucose transporter 9 expression in kidney to influence serum uric acid level in hyperuricemia mice
- Compound Tufuling Granules (复方土茯苓颗粒) regulate glucose transporter 9 expression in kidney to influence serum uric acid level in hyperuricemia mice
- 中国结合医学杂志（英文版） 2015年21卷第11期页码：823-829
- Affiliations：
  
  1. Department of Traditional Chinese Medicine, General Hospital of Guangzhou Military Command of PLA,Guangzhou,China
  2. Guangzhou University of Traditional Chinese Medicine,Guangzhou,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-015-2052-2
  中图分类号：
- 纸质出版日期：2015，
  
  网络出版日期：2015-4-11，
Scan for full text
Liu, Yw., Sun, Wf., Zhang, Xx. et al. Compound Tufuling Granules (复方土茯苓颗粒) regulate glucose transporter 9 expression in kidney to influence serum uric acid level in hyperuricemia mice., Chin. J. Integr. Med. 21, 823–829 (2015). https://doi.org/10.1007/s11655-015-2052-2

Ying-wan Liu, Wei-feng Sun, Xian-xian Zhang, et al. Compound Tufuling Granules (复方土茯苓颗粒) regulate glucose transporter 9 expression in kidney to influence serum uric acid level in hyperuricemia mice[J]. Chinese Journal of Integrative Medicine, 2015,21(11):823-829.
Liu, Yw., Sun, Wf., Zhang, Xx. et al. Compound Tufuling Granules (复方土茯苓颗粒) regulate glucose transporter 9 expression in kidney to influence serum uric acid level in hyperuricemia mice., Chin. J. Integr. Med. 21, 823–829 (2015). https://doi.org/10.1007/s11655-015-2052-2 DOI：

Ying-wan Liu, Wei-feng Sun, Xian-xian Zhang, et al. Compound Tufuling Granules (复方土茯苓颗粒) regulate glucose transporter 9 expression in kidney to influence serum uric acid level in hyperuricemia mice[J]. Chinese Journal of Integrative Medicine, 2015,21(11):823-829. DOI： 10.1007/s11655-015-2052-2.

摘要

To explore the effect of Compound Tufuling Granules (复方土茯苓颗粒

CTG) on regulating glucose transporter 9 (GLUT9) expression in the kidney to influence the uric acid excretion by the kidney and serum uric acid (SUA) level in hyperuricemia mice. Sixty Kunming male mice were randomly divided into the control group

model group

benzbromarone group

and CTG high-

middle- and lowdose groups. The yeast extract and uricase inhibition method were used to build hyperuricemia model

and the corresponding drugs were administrated on the 7th day. On the 21st day the 24-h urine was collected

on the 22nd day the blood was collected

the SUA level was detected by uricase colorimetry

and the mRNA and protein expressions of GLUT9 were detected by quantitative real-time polymerase chain reaction and Western blot

respectively. Compared with the model group

the levels of SUA and the mRNA and protein expressions of GLUT9 were signifificantly decreased

and the fraction excretion of uric acid (FEUA) was significantly increased in the CTG groups and benzbromarone group (all P<0.05). There was no significant difference in the above indicators between the CTG high-dose group and benzbromarone group (P>0.05). SUA is positively related to the GLUT9 mRNA and protein expressions in the kidney (P<0.05 or P<0.01). CTG can signifificantly reduce the SUA and increase the FEUA. In addition

CTG can effectively inhibit the mRNA and protein expressions of GLUT9 in the kidney of hyperuricemia mice to inhibit the uric acid re-absorption

promote uric acid excretion and reduce SUA.

Abstract

To explore the effect of Compound Tufuling Granules (复方土茯苓颗粒

model group

benzbromarone group

and CTG high-

middle- and lowdose groups. The yeast extract and uricase inhibition method were used to build hyperuricemia model

and the corresponding drugs were administrated on the 7th day. On the 21st day the 24-h urine was collected

on the 22nd day the blood was collected

the SUA level was detected by uricase colorimetry

and the mRNA and protein expressions of GLUT9 were detected by quantitative real-time polymerase chain reaction and Western blot

respectively. Compared with the model group

the levels of SUA and the mRNA and protein expressions of GLUT9 were signifificantly decreased

CTG can effectively inhibit the mRNA and protein expressions of GLUT9 in the kidney of hyperuricemia mice to inhibit the uric acid re-absorption

promote uric acid excretion and reduce SUA.

关键词

Compound Tufuling Granuleshyperuricemiaglucose transporter 9uric acidChinese Medicine

Keywords

Compound Tufuling Granuleshyperuricemiaglucose transporter 9uric acidChinese Medicine

references

Zhang XX, Sun WF, Xu W, Wang T. Assessment on the clinical efficacy and safety of Xiezhuo Chubi Recipe in treating hyperuricemia. Chin J Integr Tradit West Med (Chin) 2011;31:1216–1219.

Zhang XX, Sun WF, Xu W, Wang T. Study on influence of Xiezhuo Chubi Decoction on uric acid and URAT1 in hyperuricemia mice. Chin J Exp Tradit Med Formul (Chin) 2012;18:144–147.

Sun WF, Zhang XX, Sun FY, Xu W, Liang J, Feng SM, et al. MicroRNA expression patterns of the kidney in hyperuricemia mice treated with Xiezhuo Chubi Decoction. Chin J Integr Med 2011;17:35–42.

Chen GL, Zhang QL, Ma XQ, XU SY. Hyperuricemia model induced by yeast in mice. Chin Pharmacolog Bull (Chin) 2003;19:467–469.

Song LJ, Zhang XY, Xilipu D, Xi QM, Zhao P, Rehemu N. Establishment of a stable hyperuricemia and hyperuricemia renal impairment model in rats. J Xinjiang Med Univ (Chin) 2011;34:55–57.

Liu SF, Zeng XJ. Advance in the research of animal models of hyperuricemia. Basic Clin Med (Chin) 2011;31:344–347.

Pan Z, Duan FJ, Wang YH, Zhang JB, Wei XH. Effect of extract of Cortex Phellodendri and Atractylodes lancea on hyperuricemia in mice. Lishizhen Med Mater Med Res (Chin) 2008;19:112–113.

Liu XY, Zhu XJ, Guo J, Wu HW, Wang YW. The effect of different strains on mouse hyperucicemia model. Progr Modern Biomed (Chin) 2011;11:4824–4827.

Jin SR, Zheng J, Liu ST. Preliminary studies on animal models of hyperuricemic mice. J Chendu Univ Tradit Chin Med (Chin) 1999;22:49–50.

Sun XL, Wang KY, Zhang DQ. Study on the inflammatory and analgesic effects of Tufuling Injection. Chin J Tradit Med Sci Technol (Chin) 2004;11:231–232.

Xu TT, Cheng ZK, Yin L, Xu WK. Material basis research on the Rhizoma Smilacis Glabrae inhibition of xanthine oxidase. J Chin Med Mater (Chin) 2012;35:582–585.

Xia DZ, Zhang Y, Lu GY. Research progress of on flavonoids preventing hyperuricemia and gout. Chin Pharm J (Chin) 2009;44:721–723.

Chen GL, Liu HP, Han R, Xu SY. Studies on the rationality of total saponin of Rhizoma Dioscoreae Hypoglaucae combined with total saponin of Radix Achyranthis Bidentataeon reducing serum uric acid and anti-inflammatory in mice. Chin Pharmacolog Bull (Chin) 2007;23:1467–1471.

Li XL, Li W, Chen GH, Hou SL. Experimental study of Radix Achyranthis Bidentatae on promoting blood circulation. Tradit Chin Med Res (Chin) 1990;3:27–29.

Xu Q, Wang R, Xu LH, Jiang JY. Effect of Rhizoma Smilacis Glabrae on cellular immunity and humoral immunity. Chin J Immunol (Chin) 1993;9:39–42.

Chen GL, Lu HX, Wang YY, Li L. Effects and mechanisms of total saponin of Rhizoma Dioscoreae Hypoglaucae and Radix Achyranthis Bidentatae on rat with acute gouty arthritis induced by monosodium urate crystal. Pharmacol Clin Chin Mater Med (Chin) 2010;26:34–37.

Wei L, Liu JT, Gou W, Liu CG, Li XK, Wang Z. Experimental study on Fengling Gel in fighting inflammation and analgesia. Shanghai J Tradit Chin Med (Chin) 2008;42:67–69.

Anzai N, Ichida K, Jutabha P, Kimura T, Babu E, Jin CJ, et al. Plasma urate level is directly regulated by a voltagedriven urate efflux transporter URATv1 (SLC2A9) in humans. J Biol Chem 2008;283:26834–26838.

Dinour D, Gray NK, Campbell S, Shu XH, Sawyer L, Richardson W, et al. Homozygous SLC2A9 mutations cause severe renal hypouricemia. J Am Soc Nephrol 2010;21:64–72.

Caulfield MJ, Munroe PB, O'Neill D, Witkowska K, Charchar FJ, Doblado M, et al. SLC2A9 is a high-capacity urate transporter in humans. PLoS Med 2008;5:1509–1523.

Kimura T, Amonpatumrat S, Tsukada A, Fukutomi T, Jutabha P, Thammapratip T, et al. Increased expression of SLC2A9 decreases urate excretion from the kidney. Nucleos Nucleot Nucleic Acids 2011;30:1295–1301.

FULL TEXT LINK

More>

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Effect of Rhizoma Polygoni Cuspidati and Ramulus Cinnamomi compatibility on uric acid metabolism and urinary neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 in rats with hyperuricemia

Future view and development of immunology: Exploring the immunology based on Chinese medicine and culture

Cost-effectiveness analysis of combined Chinese medicine and Western medicine for ischemic stroke patients

Objective tongue inspection on 142 liver cancer patients with damp-heat syndrome

Effect of Ermiao Recipe (二妙方) with medicinal guide Angelicae Pubescentis Radix on promoting the homing of bone marrow stem cells to treat cartilage damage in osteoarthritis rats