Improvement of Kidney yang syndrome by icariin through regulating hypothalamus-pituitary-adrenal axis

Rui An; Bo Li; Li-sha You; Xin-hong Wang

doi:10.1007/s11655-015-2063-z

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Improvement of Kidney yang syndrome by icariin through regulating hypothalamus-pituitary-adrenal axis

OriginalPaper | Updated：2021-08-27

- Improvement of Kidney yang syndrome by icariin through regulating hypothalamus-pituitary-adrenal axis
- Improvement of Kidney yang syndrome by icariin through regulating hypothalamus-pituitary-adrenal axis
- 中国结合医学杂志（英文版） 2015年21卷第10期页码：765-771
- Affiliations：
  
  Department of Chemistry, College of Pharmacy, Shanghai University of Traditional Chinese Medicine,Shanghai,China
- Author bio：
- Funds：
  
  Supported by Shanghai Municipal Education Commission (No.11ZZ110 and 11YZ69), Natural Science Foundation of Shangshai (No.12ZR1431500)
- DOI：10.1007/s11655-015-2063-z
  中图分类号：
- 纸质出版日期：2015，
  
  网络出版日期：2015-3-31，
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An, R., Li, B., You, Ls. et al. Improvement of Kidney yang syndrome by icariin through regulating hypothalamus-pituitary-adrenal axis., Chin. J. Integr. Med. 21, 765–771 (2015). https://doi.org/10.1007/s11655-015-2063-z

Rui An, Bo Li, Li-sha You, et al. Improvement of Kidney yang syndrome by icariin through regulating hypothalamus-pituitary-adrenal axis[J]. Chinese Journal of Integrative Medicine, 2015,21(10):765-771.
An, R., Li, B., You, Ls. et al. Improvement of Kidney yang syndrome by icariin through regulating hypothalamus-pituitary-adrenal axis., Chin. J. Integr. Med. 21, 765–771 (2015). https://doi.org/10.1007/s11655-015-2063-z DOI：

Rui An, Bo Li, Li-sha You, et al. Improvement of Kidney yang syndrome by icariin through regulating hypothalamus-pituitary-adrenal axis[J]. Chinese Journal of Integrative Medicine, 2015,21(10):765-771. DOI： 10.1007/s11655-015-2063-z.

摘要

To investigate whether Epimedium brevicornu Maxim (EB) and icariin could exert their protective effects on hydrocortisone induced (HCI) rats by regulating the hypothalamus-pituitary-adrenal (HPA) axis and endocrine system and the possible mechanism. Male 10-week-old Sprague Dawley (SD) rats were allotted to 6 groups (A–F) with 12 each

group A was injected normal saline (NS) 3 mL/kg day intraperitoneally

group A and B were given NS 6 mL/kg day by gastrogavage

group B–F were injected hydrocortisone 15 mg/kg intraperitoneally

group C and D were given EB 8 or 5 g/(kg day) by gastrogavage

group E and F were given icariin 25 or 50 mg/(kg day) by gastrogavage. Gene expressions of hypothalamus corticotropin releasing hormone (CRH) and pituitary proopiomelanocortin (POMC) were detected by reverse transcription-polymerase chain reaction (RT-PCR)

and protein of pituitary POMC by Western-blot. The serum T4

testosterone

cortisol and POMC mRNA expression were increased after treatment with EB or icariin in HCI rats

the serum CRH and the hypothalamus CRH mRNA expression released from hypothalamus corticotropin decreased compared with group B (P<0.05).The treatment with only icariin increased serum adrenocorticotropic hormone (ACTH) compared with group B (P<0.05). EB and icariin might be therapeutically beneficial in the treatment of HCI rats through attuning the HPA axis and endocrine system which was involved in the release of CRH in hypothalamic

and the production of POMC-derived peptide ACTH in anterior pituitary

the secretion of corticosteroids in adrenal cortex.

Abstract

group A was injected normal saline (NS) 3 mL/kg day intraperitoneally

group A and B were given NS 6 mL/kg day by gastrogavage

group B–F were injected hydrocortisone 15 mg/kg intraperitoneally

group C and D were given EB 8 or 5 g/(kg day) by gastrogavage

and protein of pituitary POMC by Western-blot. The serum T4

testosterone

cortisol and POMC mRNA expression were increased after treatment with EB or icariin in HCI rats

and the production of POMC-derived peptide ACTH in anterior pituitary

the secretion of corticosteroids in adrenal cortex.

关键词

drug discoveryicariinEpimedium brevicornu Maximhypothalamus-pituitary-adrenal axisHydrocortisoneChinese Medicine

Keywords

drug discoveryicariinEpimedium brevicornu Maximhypothalamus-pituitary-adrenal axisHydrocortisoneChinese Medicine

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