Extract from Phyllanthus urinaria L. inhibits hepatitis B virus replication and expression in hepatitis B virus transfection model in vitro

Ying Wu; Ying Lu; Shu-yu Li; Yue-han Song; Yu Hao; Qian Wang

doi:10.1007/s11655-015-2076-7

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Extract from Phyllanthus urinaria L. inhibits hepatitis B virus replication and expression in hepatitis B virus transfection model in vitro

OriginalPaper | Updated：2021-08-27

- Extract from Phyllanthus urinaria L. inhibits hepatitis B virus replication and expression in hepatitis B virus transfection model in vitro
- Extract from Phyllanthus urinaria L. inhibits hepatitis B virus replication and expression in hepatitis B virus transfection model in vitro
- 中国结合医学杂志（英文版） 2015年21卷第12期页码：938-943
- Affiliations：
  
  Department of Immunology and Microbiology, Beijing University of Chinese Medicine,Beijing,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-015-2076-7
  中图分类号：
- 纸质出版日期：2015，
  
  网络出版日期：2015-4-13，
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Wu, Y., Lu, Y., Li, Sy. et al. Extract from Phyllanthus urinaria L. inhibits hepatitis B virus replication and expression in hepatitis B virus transfection model in vitro, ., Chin. J. Integr. Med. 21, 938–943 (2015). https://doi.org/10.1007/s11655-015-2076-7

Ying Wu, Ying Lu, Shu-yu Li, et al. Extract from Phyllanthus urinaria L. inhibits hepatitis B virus replication and expression in hepatitis B virus transfection model in vitro[J]. Chinese Journal of Integrative Medicine, 2015,21(12):938-943.
Wu, Y., Lu, Y., Li, Sy. et al. Extract from Phyllanthus urinaria L. inhibits hepatitis B virus replication and expression in hepatitis B virus transfection model in vitro, ., Chin. J. Integr. Med. 21, 938–943 (2015). https://doi.org/10.1007/s11655-015-2076-7 DOI：

Ying Wu, Ying Lu, Shu-yu Li, et al. Extract from Phyllanthus urinaria L. inhibits hepatitis B virus replication and expression in hepatitis B virus transfection model in vitro[J]. Chinese Journal of Integrative Medicine, 2015,21(12):938-943. DOI： 10.1007/s11655-015-2076-7.

摘要

To explore the effects of the extract from Phyllanthus urinaria L. on hepatitis B virus (HBV) replication and expression in HBV transient transfection model in vitro. The eukaryotic expression plasmid pHBV1.1

which contains 1.1-fold-overlength genome of HBV

was transfected into the human hepatoma cell line

HepG2

to establish and assess the HBV transient transfection model. The extract from Phyllanthus urinaria L. was prepared in different concentrations and methyl thiazolyl tetrazolium was used to detect the maximum nontoxic concentration of the drug. The extract from Phyllanthus urinaria L. were added into the transfected cell

at the concentrations of 0.8

0.2 and 0.05 g/L

respectively. Four days after drug application

enzyme-linked immuno sorbent assay was used to detect the concentration of HBsAg in the supernatants

Southern blot was applied to analyze HBV DNA level

and Western blot was used to detect the expression of HBcAg in cells. After the transfection of plasmid pHBV1.1 into HepG2 cells

the concentration of HBsAg in supernatants was increased obviously as compared with that of the normal cells (P<0.05)

and all expected HBV replicative intermediates were confirmed by Southern blot analysis

which ensured the successful establishment of the HBV transient transfection model. After the application of drugs at the concentrations of 0.8 and 0.2 g/L

the level of HBsAg was obviously decreased in the supernatants

as compared with that of the virus group (P<0.05); Southern blot showed that the level of HBV rc DNA

ds DNA

ss DNA was obviously reduced compared with that of the virus group (P<0.01); Western blot revealed that the expression of HBcAg in the drug group was obviously inhibited

as compared with that of the virus group (P<0.01). The extract from Phyllanthus urinaria L. obviously inhibited replication and expression of HBV in HBV transfected cell lines in vitro

thus exerting distinctive anti-HBV effects.

Abstract

which contains 1.1-fold-overlength genome of HBV

was transfected into the human hepatoma cell line

HepG2

at the concentrations of 0.8

0.2 and 0.05 g/L

respectively. Four days after drug application

enzyme-linked immuno sorbent assay was used to detect the concentration of HBsAg in the supernatants

Southern blot was applied to analyze HBV DNA level

and Western blot was used to detect the expression of HBcAg in cells. After the transfection of plasmid pHBV1.1 into HepG2 cells

the concentration of HBsAg in supernatants was increased obviously as compared with that of the normal cells (P<0.05)

and all expected HBV replicative intermediates were confirmed by Southern blot analysis

which ensured the successful establishment of the HBV transient transfection model. After the application of drugs at the concentrations of 0.8 and 0.2 g/L

the level of HBsAg was obviously decreased in the supernatants

as compared with that of the virus group (P<0.05); Southern blot showed that the level of HBV rc DNA

ds DNA

ss DNA was obviously reduced compared with that of the virus group (P<0.01); Western blot revealed that the expression of HBcAg in the drug group was obviously inhibited

as compared with that of the virus group (P<0.01). The extract from Phyllanthus urinaria L. obviously inhibited replication and expression of HBV in HBV transfected cell lines in vitro

thus exerting distinctive anti-HBV effects.

关键词

Phyllanthus urinaria L.hepatitis B virusreplicationexpressiontransient transfection modelChinese Medicine

Keywords

Phyllanthus urinaria L.hepatitis B virusreplicationexpressiontransient transfection modelChinese Medicine

references

Peng LS, He JS, Tong GD, Zhou DQ, Zhang X, Pan JB. Inhibition of extract of phyllanthus Urinaria L. on hepatitis B virus and hepatitis B X gene in vitro. Chin J Integr Trad West Med Liver Dis (Chin) 2006;16:340–343.

Thyagarajan SP, Subramanian S, Thirunalasundari T, Venkateswaran PS, Blumberg BS. Effect of Phyllanthus amarus on chronic carriers of hepatitis B virus. Lancet 1988;2:764–766.

Chen YX, Guo SH, Qi ZY, Zhang DF. Experimental study on the effect of Guangxi phyllanthus urinaria on anti-duck hepatitis B virus. J Chongqing Med Univ (Chin) 2000;25:39–40.

Tang N, Huang AL, Zhang BQ, Yan G, Xiang MQ, Pu Dan, et al. Construction of recombinant eukaryotic expression plasmid containing 1.3-fold-overlength genome of HBV and its expression in HepG2 cells. Chin J Hepatol (Chin) 2003;11:464–466.

Sprinzl MF, Oberwinkler H, Schaller H, Protzer U. Transfer of hepatitis B virus genome by adenovirus vectors into cultured cells and mice: crossing the species barrier. J Virol 2001;75:5108–5118.

Tang H, Mclachlan A. Transcriptional regulation of hepatitis B virus by nuclear hormone receptors is critical determinant of viral tropism. Proc Natl Acad Sci USA 2001;98:1841–1846.

Liu JH, Yan ZJ, Lai WH, Wei SL. Inhibitory effect of Phyllanthus urinaria L on HBV in vitro. Food Industry (Chin) 2008;29:104–106.

Dougherty AM, Guo H, Westby G, Liu Y, Simsek E, Guo JT, et al. A substituted tetrahydro-tetrazolo-pyrimidine is a specific and novel inhibitor of hepatitis B virus surface antigen secretion. Antimicrob Agents Chemother 2007;51:4427–4437.

Zhang L, Wang G, Hou W, Li P, Dulin A, Bonkovsky HL. Contemporary clinical research of traditional Chinese medicines for chronic hepatitis B in China: an analytical review. Hepatology 2010;51:690–698.

McCulloch M, Broffman M, Gao J, Colford JM. Chinese herbal medicine and interferon in the treatment of chronic hepatitis B: a meta-analysis of randomized, controlled trials. Am J Public Health 2002;92:1619–1628.

Qi FH, Wang ZX, Cai PP, Zhao L, Gao JJ, Kokudo N, et al. Traditional Chinese medicine and related active compounds: a review of their role on hepatitis B virus infection. Drug Discov Ther 2013;7:212–224.

Li M, Sun XH, Zhou ZH, Yu Z, Jin SG, Zhu XJ, et al. Beneficial therapeutic effect of Chinese herbal Bushen Formula on CHB patients with mildly elevated alanine aminotransferase by down-regulating CD4+CD25+T cells. J Ethnopharmacol 2013;146:614–622.

Zhang CP, Tian ZB, Liu XS, Zhao QX, Wu J, Liang YX. Effects of Zhaoyangwan on chronic hepatitis B and posthepatic cirrhosis. World J Gastroenterol 2004;10:295–298.

Cheng YA, Wang SD, Dang SS, Gao N, Yang Y. Clinical study of Phyllanthus Pill on treating chronic hepatitis B. Chin J Integr Trad West Med Liver Dis (Chin) 2009;19:195–197.

Guidotti LG, Matzke B, Schaller H, Chisari FV. High-level hepatitis B virus replication in transgenic mice. J Virol 1995; 69:6158–6169.

Wong GL, Wong VW, Chan HL. Combination therapy of interferon and nucleotide/nucleoside analogues for chronic hepatitis B. J Viral Hepatol 2014;21:825–834.

Song MN, Hong MZ, Luo DQ, Huang WQ, Min F, Fan RH, et al. Efficacy of 3 years of adefovir monotherapy in chronic hepatitis B patients with lamivudine resistance. World J Hepatol 2012;4:389–393.

Preda CM, Baicus C, Negreanu L, Tugui L, Olariu SV, Andrei A, et al. Effectiveness of entecavir treatment and predictive factors for virologic response. Rev Esp Enferm Dig 2014;106:305–311.

Zou S, Li XS, Li XJ. Comparision of the efficacy of two kinds of antiviral drugs adefovir and entecavir. Chin J Modern Med (Chin) 2010;20:1311–1393.

Zhang LZ, Guo YJ, Tu GZ, Guo WB, Miao F. Studies on chemical constituents of Phyllanthus urinaria L. China J Chin Mater Med (Chin) 2000;25:615–617.

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