Wenxia Changfu Formula (温下肠腑方) induces apoptosis of lung adenocarcinoma in a transplanted tumor model of drug-resistance nude mice

Xu-ming Ji; Zhi-chun Wu; Guo-wei Liu; Hua-yun Yu; Heng Liu; Zheng-tao Wang; Xiao-hui Wei; Bing Ouyang

doi:10.1007/s11655-015-2087-4

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Wenxia Changfu Formula (温下肠腑方) induces apoptosis of lung adenocarcinoma in a transplanted tumor model of drug-resistance nude mice

OriginalPaper | Updated：2021-08-27

- Wenxia Changfu Formula (温下肠腑方) induces apoptosis of lung adenocarcinoma in a transplanted tumor model of drug-resistance nude mice
- Wenxia Changfu Formula (温下肠腑方) induces apoptosis of lung adenocarcinoma in a transplanted tumor model of drug-resistance nude mice
- 中国结合医学杂志（英文版） 2016年22卷第10期页码：752-758
- Affiliations：
  
  1. School of Basic Medical Science, Shandong University of Traditional Chinese Medicine,Jinan,China
  2. Department of Neurology, Qilu Hospital, Shandong University,Jinan,China
  3. Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine,Shanghai,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81072832, 81273634), and the Opening Project of Shanghai Key Laboratory of Complex Prescription (No. 11DZ2272300), National Science and Technology Major Projects for "Major New Drugs Innovation and Development (No. 2010Z0940)
- DOI：10.1007/s11655-015-2087-4
  中图分类号：
- 纸质出版日期：2016，
  
  网络出版日期：2015-12-14，
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Ji, Xm., Wu, Zc., Liu, Gw. et al. Wenxia Changfu Formula (温下肠腑方) induces apoptosis of lung adenocarcinoma in a transplanted tumor model of drug-resistance nude mice., Chin. J. Integr. Med. 22, 752–758 (2016). https://doi.org/10.1007/s11655-015-2087-4

Xu-ming Ji, Zhi-chun Wu, Guo-wei Liu, et al. Wenxia Changfu Formula (温下肠腑方) induces apoptosis of lung adenocarcinoma in a transplanted tumor model of drug-resistance nude mice[J]. Chinese Journal of Integrative Medicine, 2016,22(10):752-758.
Ji, Xm., Wu, Zc., Liu, Gw. et al. Wenxia Changfu Formula (温下肠腑方) induces apoptosis of lung adenocarcinoma in a transplanted tumor model of drug-resistance nude mice., Chin. J. Integr. Med. 22, 752–758 (2016). https://doi.org/10.1007/s11655-015-2087-4 DOI：

Xu-ming Ji, Zhi-chun Wu, Guo-wei Liu, et al. Wenxia Changfu Formula (温下肠腑方) induces apoptosis of lung adenocarcinoma in a transplanted tumor model of drug-resistance nude mice[J]. Chinese Journal of Integrative Medicine, 2016,22(10):752-758. DOI： 10.1007/s11655-015-2087-4.

摘要

To explore the apoptosis mechanism of Wenxia Changfu Formula (温下肠腑方

WCF) in reversing drug resistance of lung cancer in vivo. Thirty model mice were randomly assigned to three groups: control group

cisplatin (CDDP) group

and WCF group. A transplanted tumor model of lung adenocarcinoma was established in all groups. Mice in the WCF group received intragastric administration of WCF (0.2 mL/10 g body weight) everyday in addition to CDDP intraperitoneally (5 mg/kg body weight) twice a week. The mice in the CDDP group received CDDP intraperitoneally (5 mg/kg body weight) twice a week

while the control group received normal saline intraperitoneally (0.2 mL/10 g body weight) everyday. The weight of the nude mice and respective tumors

tumor volume and tumor-inhibiting rate were measured. Electron microscopy was used to observe the existence of apoptosis body. Apoptosis index (AI) was detected by TdT-mediated dUTP nick end labeling staining. The expression of Fas and FasL mRNA was investigated by reverse transcription polymerase chain reaction

while immunohistochemistry was applied to detect the protein expression of Fas and FasL

caspase-3 and caspase-activated DNase (CAD)

respectively. Compared with CDDP group and control group

WCF could significantly reduce the tumor volume from the 19th day and alleviate the tumor weight (P <0.05)

and the apoptosis body was found in tumor cells in the WCF group. WCF could also enhance the level of AI

up-regulate the expression of caspase apoptosis pathway related protein caspase-3 and CAD

as well as the expression of Fas

FasL mRNA and protein (P <0.05). WCF could improve the sensitivity of tumor cells to CDDP and reverse the drug resistance by inducing the apoptosis.

Abstract

To explore the apoptosis mechanism of Wenxia Changfu Formula (温下肠腑方

WCF) in reversing drug resistance of lung cancer in vivo. Thirty model mice were randomly assigned to three groups: control group

cisplatin (CDDP) group

while the control group received normal saline intraperitoneally (0.2 mL/10 g body weight) everyday. The weight of the nude mice and respective tumors

while immunohistochemistry was applied to detect the protein expression of Fas and FasL

caspase-3 and caspase-activated DNase (CAD)

respectively. Compared with CDDP group and control group

WCF could significantly reduce the tumor volume from the 19th day and alleviate the tumor weight (P <0.05)

and the apoptosis body was found in tumor cells in the WCF group. WCF could also enhance the level of AI

up-regulate the expression of caspase apoptosis pathway related protein caspase-3 and CAD

as well as the expression of Fas

FasL mRNA and protein (P <0.05). WCF could improve the sensitivity of tumor cells to CDDP and reverse the drug resistance by inducing the apoptosis.

关键词

Wenxia Changfu Formulaanimal modeldrug resistanceapoptosisin vivo

Keywords

Wenxia Changfu Formulaanimal modeldrug resistanceapoptosisin vivo

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