Effect of Qiguiyin Decoction (芪归银方) on multidrug-resistant Pseudomonas aeruginosa infection in rats

Ling-bo Kong; Qun Ma; Jie Gao; Guo-song Qiu; Li-xia Wang; Shu-min Zhao; Yong-gang Bao; Qing-quan Liu

doi:10.1007/s11655-015-2089-2

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Effect of Qiguiyin Decoction (芪归银方) on multidrug-resistant Pseudomonas aeruginosa infection in rats

OriginalPaper | Updated：2021-08-27

- Effect of Qiguiyin Decoction (芪归银方) on multidrug-resistant Pseudomonas aeruginosa infection in rats
- Effect of Qiguiyin Decoction (芪归银方) on multidrug-resistant Pseudomonas aeruginosa infection in rats
- 中国结合医学杂志（英文版） 2015年21卷第12期页码：916-921
- Affiliations：
  
  1. Department of Emergency Medicine, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine,Beijing,China
  2. School of Chinese Materia Medica, Beijing University of Chinese Medicine,Beijing,China
  3. PepMetric Technologies Inc.,Ottawa,Canada
  4. Beijing Genovax Biotech Corperation,Beijing,China
  5. Department of Emergency Medicine, Beijing Hospital of Traditional Chinese Medicine,Beijing,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-015-2089-2
  中图分类号：
- 纸质出版日期：2015，
  
  网络出版日期：2015-9-30，
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Kong, Lb., Ma, Q., Gao, J. et al. Effect of Qiguiyin Decoction (芪归银方) on multidrug-resistant Pseudomonas aeruginosa infection in rats., Chin. J. Integr. Med. 21, 916–921 (2015). https://doi.org/10.1007/s11655-015-2089-2

Ling-bo Kong, Qun Ma, Jie Gao, et al. Effect of Qiguiyin Decoction (芪归银方) on multidrug-resistant Pseudomonas aeruginosa infection in rats[J]. Chinese Journal of Integrative Medicine, 2015,21(12):916-921.
Kong, Lb., Ma, Q., Gao, J. et al. Effect of Qiguiyin Decoction (芪归银方) on multidrug-resistant Pseudomonas aeruginosa infection in rats., Chin. J. Integr. Med. 21, 916–921 (2015). https://doi.org/10.1007/s11655-015-2089-2 DOI：

Ling-bo Kong, Qun Ma, Jie Gao, et al. Effect of Qiguiyin Decoction (芪归银方) on multidrug-resistant Pseudomonas aeruginosa infection in rats[J]. Chinese Journal of Integrative Medicine, 2015,21(12):916-921. DOI： 10.1007/s11655-015-2089-2.

摘要

To investigate the effect of Qiguiyin Decoction (芪归银方

QGYD) on multidrug-resistant Pseudomonas aeruginosa infection in Sprague-Dawley (SD) rats. A pseudomonal infection model in SD rats was established by injecting multidrug-resistant P. aeruginosa intraperitoneally. Infected rats were randomized into four groups treated with Pure water

QGYD

ceftazidime

or combined QGYD and ceftazidime. Blood samples were obtained from the abdominal aorta. Serum was then collected and analyzed by peptide array for immune responsiveness to multidrug-resistant beta-lactamase proteins

including Verona integronen-coded metallo-beta-lactamase 1 (VIM-1)

Sao Paulo metallo-beta-lactamase 1 (SPM-1)

and Temoniera (TEMs). Blood levels of interleukin-1β (IL-1β)

interleukin-4 (IL-4)

and interferon-γ (IFN-γ) were assessed by enzyme-linked immunosorbent assay. QGYD enhanced antibody reactivity against VIM-1 [epitopes 7–11 and 36–40] and TEM-1 [epitopes 26–27

52–55

and 66–70]. QGYD treatment restored the compromised antibody reactivity against VIM-1 [epitopes 53–54 and 56–58] and SPM-1 [epitopes 16–19 and 82–85] following pseudomonal infection. Serum levels of IL-1β and Th1/Th2 in the rats were significantly elevated following pseudomonal infection (P<0.05 orP<0.01). In contrast

QGYD and combination QGYD and ceftazidime treatment restored the elevated serum IL-1β and Th1/Th2 levels to normal (P>0.05). QGYD improves the immune response to pseudomonal infection in rats by stimulating the production of protective antibodies against drug-resistant proteins VIM-1

SPM-1

and TEM-1. In addition

it protects the immune system and maintains immune responsiveness by restoring IL-1β and Th1/Th2 levels.

Abstract

To investigate the effect of Qiguiyin Decoction (芪归银方

QGYD

ceftazidime

including Verona integronen-coded metallo-beta-lactamase 1 (VIM-1)

Sao Paulo metallo-beta-lactamase 1 (SPM-1)

and Temoniera (TEMs). Blood levels of interleukin-1β (IL-1β)

interleukin-4 (IL-4)

and interferon-γ (IFN-γ) were assessed by enzyme-linked immunosorbent assay. QGYD enhanced antibody reactivity against VIM-1 [epitopes 7–11 and 36–40] and TEM-1 [epitopes 26–27

52–55

SPM-1

and TEM-1. In addition

it protects the immune system and maintains immune responsiveness by restoring IL-1β and Th1/Th2 levels.

关键词

Chinese MedicineQiguiyin Decoctionmultidrug-resistantPseudomonas aeruginosapeptide array

Keywords

Chinese MedicineQiguiyin Decoctionmultidrug-resistantPseudomonas aeruginosapeptide array

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