The expression of calcitonin gene-related peptide on the neurons associated Zusanli (ST 36) in rats

Jing-jing Cui; Xin-long Zhu; Hong Shi; Yang-shuai Su; Xiang-hong Jing; Wan-zhu Bai

doi:10.1007/s11655-015-2111-8

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The expression of calcitonin gene-related peptide on the neurons associated Zusanli (ST 36) in rats

OriginalPaper | Updated：2021-08-27

- The expression of calcitonin gene-related peptide on the neurons associated Zusanli (ST 36) in rats
- The expression of calcitonin gene-related peptide on the neurons associated Zusanli (ST 36) in rats
- 中国结合医学杂志（英文版） 2015年21卷第8期页码：630-634
- Affiliations：
  
  Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81072759), the National Basic Research Program of China (973 program, No. 2011CB505201) and the Self-selected Research Program from China Academy of Chinese Medical Sciences (No. 2009Z02024)
- DOI：10.1007/s11655-015-2111-8
  中图分类号：
- 纸质出版日期：2015，
  
  网络出版日期：2015-7-31，
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Cui, Jj., Zhu, Xl., Shi, H. et al. The expression of calcitonin gene-related peptide on the neurons associated Zusanli (ST 36) in rats., Chin. J. Integr. Med. 21, 630–634 (2015). https://doi.org/10.1007/s11655-015-2111-8

Jing-jing Cui, Xin-long Zhu, Hong Shi, et al. The expression of calcitonin gene-related peptide on the neurons associated Zusanli (ST 36) in rats[J]. Chinese Journal of Integrative Medicine, 2015,21(8):630-634.
Cui, Jj., Zhu, Xl., Shi, H. et al. The expression of calcitonin gene-related peptide on the neurons associated Zusanli (ST 36) in rats., Chin. J. Integr. Med. 21, 630–634 (2015). https://doi.org/10.1007/s11655-015-2111-8 DOI：

Jing-jing Cui, Xin-long Zhu, Hong Shi, et al. The expression of calcitonin gene-related peptide on the neurons associated Zusanli (ST 36) in rats[J]. Chinese Journal of Integrative Medicine, 2015,21(8):630-634. DOI： 10.1007/s11655-015-2111-8.

摘要

To investigate the biochemical characteristic of the neurons associated Zusanli (ST 36) in the rat by using Alexa Fluor 594 conjugated cholera toxin subunit B (AF594-CTB) neural tracing and calcitonin gene-related peptide (CGRP) fluorescent immunohistochemical techniques. Four male Sprague Dawley rats were injected with AF594-CTB into the corresponding area of the Zusanli in the human body. After 3 surviving days

the rat’s spinal cord and dorsal root ganglia (DRGs) at lumbar segments were dissected following perfusion with 4% paraformaldehyde

cut into sections

and then stained with CGRPfluorescent immunohistochemical method. AF594-CTB labeled sensory neurons were detected in the L3–L6 DRGs with high concentration in L4 DRG

and the labeled motor neurons located in the dorsolateral and intermediate regions of lamina IX from L3–L5 segments with high concentration at L4. Meanwhile

CGRPpositive neural labeling distributed symmetrically on both sides of DRGs

anterior and dorsal horns of spinal cord. In the AF594-CTB labeled neurons

37% sensory neurons and 100% motor neurons expressed CGRPpositive. These findings present the morphological evidence to demonstrate that the sensory and motor neurons associated Zusanli in the rat distributed with segmental and regional patterns

and contained CGRP-expression.

Abstract

the rat’s spinal cord and dorsal root ganglia (DRGs) at lumbar segments were dissected following perfusion with 4% paraformaldehyde

cut into sections

and then stained with CGRPfluorescent immunohistochemical method. AF594-CTB labeled sensory neurons were detected in the L3–L6 DRGs with high concentration in L4 DRG

and the labeled motor neurons located in the dorsolateral and intermediate regions of lamina IX from L3–L5 segments with high concentration at L4. Meanwhile

CGRPpositive neural labeling distributed symmetrically on both sides of DRGs

anterior and dorsal horns of spinal cord. In the AF594-CTB labeled neurons

and contained CGRP-expression.

关键词

Zusanli (ST 36)neural tracingcalcitonin gene-related peptideAlexa Fluor 594 conjugated cholera toxin subunit Bsensory neuronmotor neuron

Keywords

Zusanli (ST 36)neural tracingcalcitonin gene-related peptideAlexa Fluor 594 conjugated cholera toxin subunit Bsensory neuronmotor neuron

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