Effects of acupuncture at Baihui (GV 20) and Zusanli (ST 36) on peripheral serum expression of MicroRNA 124, laminin and integrin β1 in rats with cerebral ischemia reperfusion injury

Su-hui Chen; Hua Sun; Ya-min Zhang; Hong Xu; Yang Yang; Fu-ming Wang

doi:10.1007/s11655-015-2112-7

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Effects of acupuncture at Baihui (GV 20) and Zusanli (ST 36) on peripheral serum expression of MicroRNA 124, laminin and integrin β1 in rats with cerebral ischemia reperfusion injury

OriginalPaper | Updated：2021-08-27

- Effects of acupuncture at Baihui (GV 20) and Zusanli (ST 36) on peripheral serum expression of MicroRNA 124, laminin and integrin β1 in rats with cerebral ischemia reperfusion injury
- Effects of acupuncture at Baihui (GV 20) and Zusanli (ST 36) on peripheral serum expression of MicroRNA 124, laminin and integrin β1 in rats with cerebral ischemia reperfusion injury
- 中国结合医学杂志（英文版） 2016年22卷第1期页码：49-55
- Affiliations：
  
  Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81273850)
- DOI：10.1007/s11655-015-2112-7
  中图分类号：
- 纸质出版日期：2016，
  
  网络出版日期：2015-10-1，
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Chen, Sh., Sun, H., Zhang, Ym. et al. Effects of acupuncture at Baihui (GV 20) and Zusanli (ST 36) on peripheral serum expression of MicroRNA 124, laminin and integrin β1 in rats with cerebral ischemia reperfusion injury., Chin. J. Integr. Med. 22, 49–55 (2016). https://doi.org/10.1007/s11655-015-2112-7

Su-hui Chen, Hua Sun, Ya-min Zhang, et al. Effects of acupuncture at Baihui (GV 20) and Zusanli (ST 36) on peripheral serum expression of MicroRNA 124, laminin and integrin β1 in rats with cerebral ischemia reperfusion injury[J]. Chinese Journal of Integrative Medicine, 2016,22(1):49-55.
Chen, Sh., Sun, H., Zhang, Ym. et al. Effects of acupuncture at Baihui (GV 20) and Zusanli (ST 36) on peripheral serum expression of MicroRNA 124, laminin and integrin β1 in rats with cerebral ischemia reperfusion injury., Chin. J. Integr. Med. 22, 49–55 (2016). https://doi.org/10.1007/s11655-015-2112-7 DOI：

Su-hui Chen, Hua Sun, Ya-min Zhang, et al. Effects of acupuncture at Baihui (GV 20) and Zusanli (ST 36) on peripheral serum expression of MicroRNA 124, laminin and integrin β1 in rats with cerebral ischemia reperfusion injury[J]. Chinese Journal of Integrative Medicine, 2016,22(1):49-55. DOI： 10.1007/s11655-015-2112-7.

摘要

To explore the effects of acupuncture at Baihui (GV 20) and Zusanli (ST 36) on the peripheral serum expression of microRNA 124 (miRNA 124)

laminin and integrin β1 in rats with cerebral ischemia reperfusion injury (CIRI). Seventy-two healthy male Sprague-Dawley rats were randomized into a model group

an acupuncture group

and a sham-operated group using a random digits table

with 24 rats per group. Each group was further randomly divided into 1-

and 7-day subgroups based on the reperfusion time according to a random digits table

with 6 rats in each subgroup. In the model and acupuncture groups

CIRI was induced using the thread occlusion method. Electroacupuncture stimulation was applied daily to GV 20 and left ST 36 for 20 min at the indicated time points after successful operations. Serum was sampled for detecting laminin and integrin β1 protein via enzyme-linked immunosorbent assay

and serum miRNA 124 was examined using quantitative polymerase chain reaction. The serum level of miRNA 124 in the cerebral ischemia rats increased significantly

and the peak expression of miRNA 124 in both the model and acupuncture groups occurred at 3 days. The expression of miRNA 124 in the acupuncture group was higher than in the model group at the same time point (5.96±0.01 vs. 3.11±0.04

P <0.05). Laminin expression in serum from the cerebral ischemia group was higher than that in the sham-operated group. Compared with the model group

the level of laminin in the serum of the acupuncture group was significantly lower at each time point

especially at the 3-day

and 7-day time points (589.12±3.57 vs. 793.05±5.28

and 600.53±3.05 vs. 899.06±5.74

P <0.05). The level of integrin β1 in the serum from the acupuncture group was lower than that in the model group particularly at the 3-day and 7-day time points (208.66±0.95 vs. 280.83±1.77

and 212.36±0.95 vs. 316.77±2.42

P <0.05). Additionally

the model group and the acupuncture group showed dual peaks of integrin β1 and laminin expression at 3-day and 7-day. Acupuncture at GV 20 and ST 36 in rats alleviated CIRI and was associated with upregulated expression of miRNA 124 and with downregulated expression of integrin β1 and laminin in peripheral serum. These changes may represent one of the mechanisms underlying acupuncture’s attenuation of CIRI.

Abstract

To explore the effects of acupuncture at Baihui (GV 20) and Zusanli (ST 36) on the peripheral serum expression of microRNA 124 (miRNA 124)

laminin and integrin β1 in rats with cerebral ischemia reperfusion injury (CIRI). Seventy-two healthy male Sprague-Dawley rats were randomized into a model group

an acupuncture group

and a sham-operated group using a random digits table

with 24 rats per group. Each group was further randomly divided into 1-

and 7-day subgroups based on the reperfusion time according to a random digits table

with 6 rats in each subgroup. In the model and acupuncture groups

and serum miRNA 124 was examined using quantitative polymerase chain reaction. The serum level of miRNA 124 in the cerebral ischemia rats increased significantly

P <0.05). Laminin expression in serum from the cerebral ischemia group was higher than that in the sham-operated group. Compared with the model group

the level of laminin in the serum of the acupuncture group was significantly lower at each time point

especially at the 3-day

and 7-day time points (589.12±3.57 vs. 793.05±5.28

and 600.53±3.05 vs. 899.06±5.74

P <0.05). The level of integrin β1 in the serum from the acupuncture group was lower than that in the model group particularly at the 3-day and 7-day time points (208.66±0.95 vs. 280.83±1.77

and 212.36±0.95 vs. 316.77±2.42

P <0.05). Additionally

关键词

acupuncturecerebral ischemia reperfusion injurymicroRNA 124lamininintegrin β1

Keywords

acupuncturecerebral ischemia reperfusion injurymicroRNA 124lamininintegrin β1

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