Icariin protects SH-SY5Y cells from formaldehyde-induced injury through suppression of Tau phosphorylation

Yi-xiang Song; Jun-ye Miao; Min Qiang; Rong-qiao He; Xue-mei Wang; Wei-wei Li

doi:10.1007/s11655-015-2116-3

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Icariin protects SH-SY5Y cells from formaldehyde-induced injury through suppression of Tau phosphorylation

OriginalPaper | Updated：2021-08-27

- Icariin protects SH-SY5Y cells from formaldehyde-induced injury through suppression of Tau phosphorylation
- Icariin protects SH-SY5Y cells from formaldehyde-induced injury through suppression of Tau phosphorylation
- 中国结合医学杂志（英文版） 2016年22卷第6期页码：430-437
- Affiliations：
  
  1. Integrated Laboratory of Chinese and Western Medicine, Peking University First Hospital,Beijing,China
  2. State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81102683 and No. 81173369), the Doctoral Fellowships of Ministry of Education of China (No. 20120001110105 and No. 20110001120055) and Beijing Natural Science Foundation (No. 7132210 )
- DOI：10.1007/s11655-015-2116-3
  中图分类号：
- 纸质出版日期：2016，
  
  网络出版日期：2015-11-20，
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Song, Yx., Miao, Jy., Qiang, M. et al. Icariin protects SH-SY5Y cells from formaldehyde-induced injury through suppression of Tau phosphorylation., Chin. J. Integr. Med. 22, 430–437 (2016). https://doi.org/10.1007/s11655-015-2116-3

Yi-xiang Song, Jun-ye Miao, Min Qiang, et al. Icariin protects SH-SY5Y cells from formaldehyde-induced injury through suppression of Tau phosphorylation[J]. Chinese Journal of Integrative Medicine, 2016,22(6):430-437.
Song, Yx., Miao, Jy., Qiang, M. et al. Icariin protects SH-SY5Y cells from formaldehyde-induced injury through suppression of Tau phosphorylation., Chin. J. Integr. Med. 22, 430–437 (2016). https://doi.org/10.1007/s11655-015-2116-3 DOI：

Yi-xiang Song, Jun-ye Miao, Min Qiang, et al. Icariin protects SH-SY5Y cells from formaldehyde-induced injury through suppression of Tau phosphorylation[J]. Chinese Journal of Integrative Medicine, 2016,22(6):430-437. DOI： 10.1007/s11655-015-2116-3.

摘要

To investigate the neuroprotective effects of icariin on formaldehyde (FA)-treated human neuroblastoma SH-SY5Y cells and the possible mechanisms involved. SH-SY5Y cells were divided into FA treatment group

FA treatment group with icariin

and the control group. Cell viability

apoptosis

and morphological changes were determined by cell counting kit-8 (CCK 8)

flow cytometry

and confocal microscopy

respectively. The phosphorylation of Tau protein was examined by western blotting. FA showed a half lethal dose (LD50) of 0.3 mmol/L in SH-SY5Y cells under the experimental conditions. Icariin (1–10 µmol/L) prevented FA-induced cell death in SH-SY5Y cells in a dose-dependent manner

with the optimal effect observed at 5 µmol/L. After FA treatment

the absorbance in FA group was 1.31±0.05

while in the group of icariin (5 µmol/L) was 1.63±0.05. Examination of cell morphology by confocal microscopy demonstrated that 5 µmol/L icariin significantly attenuated FA-induced cell injury (P <0.05). Additionally

Icariin inhibited FA-induced cell apoptosis in SH-SY5Y cells. Results from western blotting showed that icariin suppressed FA-induced phosphorylation at Thr 181 and Ser 396 of Tau protein

while having no effect on the expression of the total Tau protein level. Furthermore

FA activated Tau kinase glycogen synthase kinase 3 beta (GSK-3β) by enhancement of Y216 phosphorylation

but icariin reduced Y216 phosphorylation and increased Ser 9 phosphorylation. Icariin protects SH-SY5Y cells from FA-induced injury poßsibly through the inhibition of GSK-3β-mediated Tau phosphorylation.

Abstract

FA treatment group with icariin

and the control group. Cell viability

apoptosis

and morphological changes were determined by cell counting kit-8 (CCK 8)

flow cytometry

and confocal microscopy

with the optimal effect observed at 5 µmol/L. After FA treatment

the absorbance in FA group was 1.31±0.05

Icariin inhibited FA-induced cell apoptosis in SH-SY5Y cells. Results from western blotting showed that icariin suppressed FA-induced phosphorylation at Thr 181 and Ser 396 of Tau protein

while having no effect on the expression of the total Tau protein level. Furthermore

FA activated Tau kinase glycogen synthase kinase 3 beta (GSK-3β) by enhancement of Y216 phosphorylation

关键词

icariinformaldehydeTau phosphorylationglycogen synthase kinase 3 betaneuroprotection

Keywords

icariinformaldehydeTau phosphorylationglycogen synthase kinase 3 betaneuroprotection

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