Effects of Weipixiao (胃痞消) on Wnt pathway-associated proteins in gastric mucosal epithelial cells from rats with gastric precancerous lesions

Jin-hao Zeng; Hua-feng Pan; You-zhang Liu; Hai-bo Xu; Zi-ming Zhao; Hai-wen Li; Jin-ling Ren; Long-hui Chen; Xia Hu; Yan Yan

doi:10.1007/s11655-015-2131-4

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Effects of Weipixiao (胃痞消) on Wnt pathway-associated proteins in gastric mucosal epithelial cells from rats with gastric precancerous lesions

OriginalPaper | Updated：2021-08-27

- Effects of Weipixiao (胃痞消) on Wnt pathway-associated proteins in gastric mucosal epithelial cells from rats with gastric precancerous lesions
- Effects of Weipixiao (胃痞消) on Wnt pathway-associated proteins in gastric mucosal epithelial cells from rats with gastric precancerous lesions
- 中国结合医学杂志（英文版） 2016年22卷第4期页码：267-275
- Affiliations：
  
  1. The First Clinical Medical College, Guangzhou University of Chinese Medicine,Guangzhou,China
  2. Department of Pharmacology, Chengdu University of Traditional Chinese Medicine,Chengdu,China
  3. Guangdong Provincial Institute of Chinese Medicine,Guangzhou,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81273739)
- DOI：10.1007/s11655-015-2131-4
  中图分类号：
- 纸质出版日期：2016，
  
  网络出版日期：2015-4-14，
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Zeng, Jh., Pan, Hf., Liu, Yz. et al. Effects of Weipixiao (胃痞消) on Wnt pathway-associated proteins in gastric mucosal epithelial cells from rats with gastric precancerous lesions., Chin. J. Integr. Med. 22, 267–275 (2016). https://doi.org/10.1007/s11655-015-2131-4

Jin-hao Zeng, Hua-feng Pan, You-zhang Liu, et al. Effects of Weipixiao (胃痞消) on Wnt pathway-associated proteins in gastric mucosal epithelial cells from rats with gastric precancerous lesions[J]. Chinese Journal of Integrative Medicine, 2016,22(4):267-275.
Zeng, Jh., Pan, Hf., Liu, Yz. et al. Effects of Weipixiao (胃痞消) on Wnt pathway-associated proteins in gastric mucosal epithelial cells from rats with gastric precancerous lesions., Chin. J. Integr. Med. 22, 267–275 (2016). https://doi.org/10.1007/s11655-015-2131-4 DOI：

Jin-hao Zeng, Hua-feng Pan, You-zhang Liu, et al. Effects of Weipixiao (胃痞消) on Wnt pathway-associated proteins in gastric mucosal epithelial cells from rats with gastric precancerous lesions[J]. Chinese Journal of Integrative Medicine, 2016,22(4):267-275. DOI： 10.1007/s11655-015-2131-4.

摘要

To study the effects of Weipixiao (胃痞消

WPX) on Wnt pathway-associated proteins in gastric mucosal epithelial cells from rats with gastric precancerous lesions (GPL). Sprague Dawley rats were randomly divided into control

model

vitacoenzyme (0.2 g·kg−1·day−1)

WPX high-dose (H-WPX

15 g·kg−1·day−1)

WPX medium-dose (M-WPX

7.5 g·kg−1·day−1) and WPX low-dose (L-WPX

3.75 g·kg−1·day−1) groups. After successfully establishing the GPL model

the rats were consecutively administered WPX or vitacoenzyme by gastrogavage for 10 weeks. Differential expression of Leucine-rich repeat-containing G-proteincoupled receptor 5 (Lgr5)

matrix metalloproteinase-7 (MMP-7)

Wnt1

Wnt3a

and β-catenin in gastric mucosal epithelial cells in all groups were immunohistochemically detected

and the images were taken and analyzed semiquantitatively by image pro plus 6.0 software. Gastric epithelium in the model group showed significantly higher expression levels of Lgr5

MMP-7

Wnt1

Wnt3a and β-catenin than those of the control group(P<0.01). Interestingly

we also observed Lgr5+ cells

which generally located at the base of the gastric glandular unit

migrated to the luminal side of gastric epithelium with GPL. The expression levels of Lgr5

MMP-7

Wnt1

and β-catenin were all down-regulated in the L-WPX group as compared with those of both model and vitacoenzyme groups (P<0.05). A similar

but nonsignificant down-regulation in expression level of Wnt3a was noted in all WPX groups (P>0.05). Our findings suggested that the therapeutic mechanisms of WPX in treating GPL might be related with its inhibitory effects on the expressions of Lgr5

MMP-7

Wnt1

β-catenin and the aberrant activation of Wnt/β-catenin pathway.

Abstract

To study the effects of Weipixiao (胃痞消

WPX) on Wnt pathway-associated proteins in gastric mucosal epithelial cells from rats with gastric precancerous lesions (GPL). Sprague Dawley rats were randomly divided into control

model

vitacoenzyme (0.2 g·kg−1·day−1)

WPX high-dose (H-WPX

15 g·kg−1·day−1)

WPX medium-dose (M-WPX

7.5 g·kg−1·day−1) and WPX low-dose (L-WPX

3.75 g·kg−1·day−1) groups. After successfully establishing the GPL model

the rats were consecutively administered WPX or vitacoenzyme by gastrogavage for 10 weeks. Differential expression of Leucine-rich repeat-containing G-proteincoupled receptor 5 (Lgr5)

matrix metalloproteinase-7 (MMP-7)

Wnt1

Wnt3a

and β-catenin in gastric mucosal epithelial cells in all groups were immunohistochemically detected

and the images were taken and analyzed semiquantitatively by image pro plus 6.0 software. Gastric epithelium in the model group showed significantly higher expression levels of Lgr5

MMP-7

Wnt1

Wnt3a and β-catenin than those of the control group(P<0.01). Interestingly

we also observed Lgr5+ cells

which generally located at the base of the gastric glandular unit

migrated to the luminal side of gastric epithelium with GPL. The expression levels of Lgr5

MMP-7

Wnt1

and β-catenin were all down-regulated in the L-WPX group as compared with those of both model and vitacoenzyme groups (P<0.05). A similar

MMP-7

Wnt1

β-catenin and the aberrant activation of Wnt/β-catenin pathway.

关键词

gastric precancerous lesionsWnt/ß-catenin pathwayLgr5matrix metalloproteinase-7Wnt1Wnt3aß-cateninWeipixiaoChinese Medicine

Keywords

gastric precancerous lesionsWnt/ß-catenin pathwayLgr5matrix metalloproteinase-7Wnt1Wnt3aß-cateninWeipixiaoChinese Medicine

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