Effects on the tumor specific growth factor and tumor necrosis factor α in rats’ precancerous lesion of primary hepatocellular carcinoma by direct moxibustion at Ganshu (BL 18) acupoint

Pei Wang; Jiang Zhu; Xi-liang Xie; Ming-he Sui; Qiu-ju Zhang; Wen-rui Jia; Si-yuan Xin; Yang Liu; Zhong-wei Hou

doi:10.1007/s11655-015-2157-7

Your Location：

Home >

Browse articles >

Effects on the tumor specific growth factor and tumor necrosis factor α in rats’ precancerous lesion of primary hepatocellular carcinoma by direct moxibustion at Ganshu (BL 18) acupoint

OriginalPaper | Updated：2021-08-27

- Effects on the tumor specific growth factor and tumor necrosis factor α in rats’ precancerous lesion of primary hepatocellular carcinoma by direct moxibustion at Ganshu (BL 18) acupoint
- Effects on the tumor specific growth factor and tumor necrosis factor α in rats’ precancerous lesion of primary hepatocellular carcinoma by direct moxibustion at Ganshu (BL 18) acupoint
- 中国结合医学杂志（英文版） 2016年22卷第7期页码：532-536
- Affiliations：
  
  1. School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine,Beijing,China
  2. The Key Unit of Evaluation of Characteristic Acupuncture Therapy, State Administration of Traditional Chinese Medicine,Beijing,China
  3. The Research Institute of Acupuncture and Moxibustion in Houma City, Houma,Shanxi Province,China
  4. School of Preclinical Medicine, Beijing University of Chinese Medicine,Beijing,China
  5. Chengde Medical College,Hebei Province,Chengde,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81202760) and the Doctoral Program of Higher Educition of Ministry Education of China (No. 20100013120014)
- DOI：10.1007/s11655-015-2157-7
  中图分类号：
- 纸质出版日期：2016，
  
  网络出版日期：2015-8-11，
Scan for full text
Wang, P., Zhu, J., Xie, Xl. et al. Effects on the tumor specific growth factor and tumor necrosis factor α in rats’ precancerous lesion of primary hepatocellular carcinoma by direct moxibustion at Ganshu (BL 18) acupoint., Chin. J. Integr. Med. 22, 532–536 (2016). https://doi.org/10.1007/s11655-015-2157-7

Pei Wang, Jiang Zhu, Xi-liang Xie, et al. Effects on the tumor specific growth factor and tumor necrosis factor α in rats’ precancerous lesion of primary hepatocellular carcinoma by direct moxibustion at Ganshu (BL 18) acupoint[J]. Chinese Journal of Integrative Medicine, 2016,22(7):532-536.
Wang, P., Zhu, J., Xie, Xl. et al. Effects on the tumor specific growth factor and tumor necrosis factor α in rats’ precancerous lesion of primary hepatocellular carcinoma by direct moxibustion at Ganshu (BL 18) acupoint., Chin. J. Integr. Med. 22, 532–536 (2016). https://doi.org/10.1007/s11655-015-2157-7 DOI：

Pei Wang, Jiang Zhu, Xi-liang Xie, et al. Effects on the tumor specific growth factor and tumor necrosis factor α in rats’ precancerous lesion of primary hepatocellular carcinoma by direct moxibustion at Ganshu (BL 18) acupoint[J]. Chinese Journal of Integrative Medicine, 2016,22(7):532-536. DOI： 10.1007/s11655-015-2157-7.

摘要

To investigate the effect of direct moxibustion at Ganshu (BL18) on the serum concentrations of tumor specific growth factor (TSGF) and tumor necrosis factor α (TNF-α) in a rat model with precancerous lesion of primary hepatocellular carcinoma (HCC)

so as to explore the mechanism of moxibustion underlying improvement of HCC. Sixty male Wistar rats were randomly divided into control group (n=10)

model group (n=20)

prevention group 1 (n=15) and prevention group 2 (n=15). The normal rats were injected with physiological saline as blank control. At the same time

the rats of other three groups were injected with diethylnitrosamine to establish the HCC model. Direct moxibustion with grain-sized moxa was applied to bilateral Ganshu acupoint of the rats in the prevention group 1 (1 treatment course

20 days) and prevention group 2 (2 treatment courses

40 days)

5 doses for each acupoint

0.5 mg/dose

once every other day. At each time point (before model establishment

the end of 1st course prevention

the end of 2nd course prevention and the end of model establishment)

serum levels of TSGF and TNF-α were detected using enzyme-linked immunosorbent assay. Compared with the control group

there was a remarkably increase of serum TSGF and TNF-α contents in the model group at the end of the experiment (P<0.05). At the end of the 1st course of direct moxibustion

the contents of serum TSGF and TNF-α of rats in the prevention group 1 were significantly increased compared with that of the model group (P<0.05). At the end of the 2nd course of direct moxibustion

serum TSGF and TNF-α levels of rats in the model group were higher than the normal group with significantly difference (P<0.05)

and the levels of TSGF and TNF-α in the prevention group 2 were significantly reduced in comparison with the model group (P<0.05). It was possible that direct moxibustion could inhibit precancerous lesion and postpone hepatocarcinogenesis

and the therapeutic effect of two courses were better than one course.

Abstract

so as to explore the mechanism of moxibustion underlying improvement of HCC. Sixty male Wistar rats were randomly divided into control group (n=10)

model group (n=20)

prevention group 1 (n=15) and prevention group 2 (n=15). The normal rats were injected with physiological saline as blank control. At the same time

20 days) and prevention group 2 (2 treatment courses

40 days)

5 doses for each acupoint

0.5 mg/dose

once every other day. At each time point (before model establishment

the end of 1st course prevention

the end of 2nd course prevention and the end of model establishment)

serum levels of TSGF and TNF-α were detected using enzyme-linked immunosorbent assay. Compared with the control group

there was a remarkably increase of serum TSGF and TNF-α contents in the model group at the end of the experiment (P<0.05). At the end of the 1st course of direct moxibustion

the contents of serum TSGF and TNF-α of rats in the prevention group 1 were significantly increased compared with that of the model group (P<0.05). At the end of the 2nd course of direct moxibustion

serum TSGF and TNF-α levels of rats in the model group were higher than the normal group with significantly difference (P<0.05)

and the therapeutic effect of two courses were better than one course.

关键词

direct moxibustionDiethylnitrosaminehepatocellular carcinomatumor specific growth factortumor necrosis factor αChinese Medicine

Keywords

direct moxibustionDiethylnitrosaminehepatocellular carcinomatumor specific growth factortumor necrosis factor αChinese Medicine

references

Teo EK, Fock KM. Hepatocellular carcinoma: an Asian perspective. Dig Dis 2001;19:263–268.

McGlynn KA, London WT. Epidemiology and natural history of hepatocellular carcinoma. Best Pract Res Clin Gastroenterol 2005;19:3–23.

Dong XJ, Shao YM. Advancement in treatment of primary liver cancer. Modern Oncol (Chin) 2009;17:1814–1817.

Xie XL. Treatment of hepatitis B: great value of direct moxibustion. Modern Health Preserv (Chin) 2008;9:18–19.

Xiao J, Wang TF. Cases of direct moxibustion by Prof. XIE Xi-liang. J Chin Acupunct Moxibust (Chin) 2008;8:527–528.

Xie XL. Cases of treatment of hepatitis B with direct moxibustion. China News Tradit Chin Med (Chin) 2005;10:10007.

Song YJ, Liu XT, Feng LL, Wang X, Bai HF, Tian MQ, et al. Defination of diethylnitrosamine-induced rat hepatic percancerous lesions with atypia index. World J Gastroenterol (Chin) 2012;20:2562–2569.

Li ZR, Fang JQ, eds. Study of experimental acupuncture and moxibustion. Beijing: China Press of Traditional Chinese Medicine; 2007:244.

Liu Y, Hou ZW, Lu J, Dong F, Wang P, Jia WR, et al. Effect of moxibustion at "Ganshu" on liver function in rat’s precancerous lesion of DEN-induced hepatocellular carcinoma. Chin Acupunct (Chin) 2015;35:702–706.

Benhaim L, Loupakis F, Labonte MJ, Lenz HJ. Selecting the best targeted agent in first-line treatment of unresectable liver metastases from colorectal cancer: does the bench have the answers? J Hepatobil Pancreat Sci 2012;19:528–535.

Yu DS, Huang XE, Zhou JN. Comparative study on the value of anal preserving surgery for aged people with low rectal carcinoma in Jiangsu, China. Asian Pacific J Cancer Prev 2012;13:2339–2340.

De CEM, Kwakmanr Van EM, Bosch LJ, Bonjer HJ, Meijer GA, et al. Understanding molecular mechanisms in peritoneal dissemination of colorectal cancer: future possibilities for personalised treatment by use of biomarkers. Virchows Arch 2012;461:231–243.

Duffy MJ. Tumor markers in clinical practice: a review focusing on common solid cancers. Med Princ Pract 2013;22:4–11.

Efferth T. Signal transduction pathways of the epidermal growth factor receptor in colorectal cancer and their inhibition by small molecules. Curr Med Chem 2012;19:5735–5744.

Qi XL, Quan LQ, Zhang HX, Zhao Y, Dong JZ. Evaluation on clinical application of malignant tumor specific growth factor (TSGF) assay. North West Milit Med J (Chin) 2005;26:248–249.

Wang JY, Dai SQ, Rong TH, Long H, Chen QL, Wang CM. Value of malignant tumor related substance in peripheral blood in diagnosis of esophageal cancer. World J Gastroenterol (Chin) 2005;13:546–547.

Chen M, Wu BP. Determination of tumor specific growth factor and its clinical value. Hebei J Integr Tradit Chin West Med (Chin) 1999;8:348–349.

Gta-Lazaro JF, Thieringer F, Luth S, Czochra P, Meyer E, Renteria IB, et al. Hepatic overexpression of TSGT promotes LPS induces inflammatory cytokine secretion by cells and erdotoxemic shock. Immunol Lett 2005;101:217–222.

Serra R, Crowley MR. Mouse models of transforming growth factor impact in breast development and cancer. Endocr Relat Cancer 2005;12:749–760.

Wang Q, Yang XZ. Primary liver cancer combined detection of serum tumor markers and their clinical significance. Fujian Med J (Chin) 2005;27:119–120.

Peterson TC, Isbrucher RA. Fibroproliferation in liver disease: role of monocyte factor. Hepatology 1992;15:191–197.

Wang LZ, Shi TX, Hu FL. Implication of determining serum interleukin-18 and tumor necrosis factor-a in infants with cytomegalovirus hepatitis. J Appl Clin Pediatr (Chin) 2005;20:433–434.

Wang GH, Xu RJ, Zhang ZS, Zhao JW, Shi YQ. Discussion on physiological and pathological role of tumor necrosis factor a. J Radioimmunol (Chin) 1999;12:259–261.

Shi XM, ed. Study of acupuncture and moxibustion. Beijing: China Press of Traditional Chinese Medicine; 2010:152–153.

FULL TEXT LINK

More>

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Preventive effects of Jiedu Granules (解毒颗粒) combined with Cinobufacini Injection (华蟾素注射液) versus transcatheter arterial chemoembolization in post-surgical patients with hepatocellular carcinoma: A case-control trial

Bear bile powder inhibits angiogenesis in vivo and in vitro

Chinese medicine herbal treatment based on syndrome differentiation improves the overall survival of patients with unresectable hepatocellular carcinoma

Future view and development of immunology: Exploring the immunology based on Chinese medicine and culture

Cost-effectiveness analysis of combined Chinese medicine and Western medicine for ischemic stroke patients