Effect of ligustrazine nanoparticles nano spray on transforming growth factor-β/Smad signal pathway of rat peritoneal mesothelial cells induced by tumor necrosis factor-α

Shuai Yan; Lan Yang; Yin-zi Yue; Wen-lin Li; Li Zeng; Jin Yue; Chun-qin Mao

doi:10.1007/s11655-015-2180-8

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Effect of ligustrazine nanoparticles nano spray on transforming growth factor-β/Smad signal pathway of rat peritoneal mesothelial cells induced by tumor necrosis factor-α

OriginalPaper | Updated：2021-08-27

- Effect of ligustrazine nanoparticles nano spray on transforming growth factor-β/Smad signal pathway of rat peritoneal mesothelial cells induced by tumor necrosis factor-α
- Effect of ligustrazine nanoparticles nano spray on transforming growth factor-β/Smad signal pathway of rat peritoneal mesothelial cells induced by tumor necrosis factor-α
- 中国结合医学杂志（英文版） 2016年22卷第8期页码：629-634
- Affiliations：
  
  1. Suzhou Hospital of Traditional Chinese Medicine,Jiangsu Province,Suzhou,China
  2. First Clinical Medical College of Nanjing University of Chinese Medicine,Nanjing,China
  3. Library of Nanjing University of Chinese Medicine,Nanjing,China
  4. Yancheng Hospital of Traditional Chinese Medicine,Jiangsu Province,Yancheng,China
  5. College of Pharmacy of Nanjing University of Chinese Medicine,Nanjing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81373843) and College Graduate Student Innovative Research Fund of Jiangsu Province, China (No. CXZZ13-0613) and Administration of Traditional Chinese Medicine Science and Technology Project of Jiangsu Province, China (No. LZ13006)
- DOI：10.1007/s11655-015-2180-8
  中图分类号：
- 纸质出版日期：2016，
  
  网络出版日期：2015-9-30，
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Yan, S., Yang, L., Yue, Yz. et al. Effect of ligustrazine nanoparticles nano spray on transforming growth factor-β/Smad signal pathway of rat peritoneal mesothelial cells induced by tumor necrosis factor-α., Chin. J. Integr. Med. 22, 629–634 (2016). https://doi.org/10.1007/s11655-015-2180-8

Shuai Yan, Lan Yang, Yin-zi Yue, et al. Effect of ligustrazine nanoparticles nano spray on transforming growth factor-β/Smad signal pathway of rat peritoneal mesothelial cells induced by tumor necrosis factor-α[J]. Chinese Journal of Integrative Medicine, 2016,22(8):629-634.
Yan, S., Yang, L., Yue, Yz. et al. Effect of ligustrazine nanoparticles nano spray on transforming growth factor-β/Smad signal pathway of rat peritoneal mesothelial cells induced by tumor necrosis factor-α., Chin. J. Integr. Med. 22, 629–634 (2016). https://doi.org/10.1007/s11655-015-2180-8 DOI：

Shuai Yan, Lan Yang, Yin-zi Yue, et al. Effect of ligustrazine nanoparticles nano spray on transforming growth factor-β/Smad signal pathway of rat peritoneal mesothelial cells induced by tumor necrosis factor-α[J]. Chinese Journal of Integrative Medicine, 2016,22(8):629-634. DOI： 10.1007/s11655-015-2180-8.

摘要

To study the effect of ligustrazine nanoparticles nano spray (LNNS) on transforming growth factor β (TGF-β)/Smad signal protein of rat peritoneal mesothelial cells (RPMC) induced by tumor necrosis factor α (TNF-α)

and the anti-adhesion mechanism of LNNS in the abdominal cavity. The primary culture and subculture of rat peritoneal mesothelial cells (RPMC) was processed by trypsin digestion method in vitro. The third generation was identifified for experiment and divided into 5 groups: a blank group: RPMC without treatment; a control group: RPMC stimulated with TNF-α; RPMC treated by a low-dosage LNNS group (2.5 mg/L); RPMC treated by a medium-dosage LNNS group (5 mg/L); and RPMC treated by a high-dosage LNNS group (10 mg/L). Reverse transcription-polymerase chain reaction was applied to test the expression of fifibronectin

collagen I (COL-I)

TGF-β mRNA

and Western blot method to test the Smad protein 7 expression of RPMC. Compared with the blank group

a signifificant elevation in fifibronectin (FN)

COL-I and TGF-β mRNA expression of RPMC were observed in the control group (P<0.05). Compared with the control group

LNNS suppressed the expressions of FN

COL-I and TGF-β mRNA in a concentrationdependent manner (P<0.05). The expression of Smad7 protein of RPMC was down-regulated by TNF-α stimulation

and up-regulated with the increase of LNNS dose (P<0.05). TNF-α may induce changes in RPMC’s viability

leading to peritoneal injury. LNNS could reverse the induction of fifibrosis related cytokine FN

COL-I and TGF-β

up-regulating the expression of Smad7 by TNF-α in RPMC

thus attenuate peritoneal injury by repairing mesothelial cells.

Abstract

collagen I (COL-I)

TGF-β mRNA

and Western blot method to test the Smad protein 7 expression of RPMC. Compared with the blank group

a signifificant elevation in fifibronectin (FN)

COL-I and TGF-β mRNA expression of RPMC were observed in the control group (P<0.05). Compared with the control group

LNNS suppressed the expressions of FN

COL-I and TGF-β mRNA in a concentrationdependent manner (P<0.05). The expression of Smad7 protein of RPMC was down-regulated by TNF-α stimulation

and up-regulated with the increase of LNNS dose (P<0.05). TNF-α may induce changes in RPMC’s viability

leading to peritoneal injury. LNNS could reverse the induction of fifibrosis related cytokine FN

COL-I and TGF-β

up-regulating the expression of Smad7 by TNF-α in RPMC

thus attenuate peritoneal injury by repairing mesothelial cells.

关键词

peritoneal mesothelial cellsLigustrazineexperimental researchtransforming growth factor β/Smad

Keywords

peritoneal mesothelial cellsLigustrazineexperimental researchtransforming growth factor β/Smad

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