Effects of agkistrodon in different dosage forms on collagen-induced arthritis in rats

Jie Bao; Zhi-jun Xie; Lei-ming Chen; Jing Sun; Yong-sheng Fan

doi:10.1007/s11655-015-2281-4

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Effects of agkistrodon in different dosage forms on collagen-induced arthritis in rats

OriginalPaper | Updated：2021-08-27

- Effects of agkistrodon in different dosage forms on collagen-induced arthritis in rats
- Effects of agkistrodon in different dosage forms on collagen-induced arthritis in rats
- 中国结合医学杂志（英文版） 2016年22卷第12期页码：902-909
- Affiliations：
  
  1. Laboratory of Rheumatoid Immune, The Second Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou,China
  2. Zhejiang Xinhua Hospital,Hangzhou,China
- Author bio：
- Funds：
  
  Supported by the Traditional Chinese Medicine Scientific Research Fund of Zhejiang Province, China (No. 2013ZA063 and No. 2012ZB062), the Key Scientific and Technological Innovation Team Plan of Zhejiang Province, China (No. 2009R50042-002) and the Third Level of 151 Talents Project of Zhejiang Province, China
- DOI：10.1007/s11655-015-2281-4
  中图分类号：
- 纸质出版日期：2016，
  
  网络出版日期：2015-8-11，
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Bao, J., Xie, Zj., Chen, Lm. et al. Effects of agkistrodon in different dosage forms on collagen-induced arthritis in rats., Chin. J. Integr. Med. 22, 902–909 (2016). https://doi.org/10.1007/s11655-015-2281-4

Jie Bao, Zhi-jun Xie, Lei-ming Chen, et al. Effects of agkistrodon in different dosage forms on collagen-induced arthritis in rats[J]. Chinese Journal of Integrative Medicine, 2016,22(12):902-909.
Bao, J., Xie, Zj., Chen, Lm. et al. Effects of agkistrodon in different dosage forms on collagen-induced arthritis in rats., Chin. J. Integr. Med. 22, 902–909 (2016). https://doi.org/10.1007/s11655-015-2281-4 DOI：

Jie Bao, Zhi-jun Xie, Lei-ming Chen, et al. Effects of agkistrodon in different dosage forms on collagen-induced arthritis in rats[J]. Chinese Journal of Integrative Medicine, 2016,22(12):902-909. DOI： 10.1007/s11655-015-2281-4.

摘要

To determine the effective dosage and formulation of agkistrodon in collagen-induced arthritis (CIA) rats. CIA was induced by injection of collagen in complete/incomplete Freund’s adjuvant. Agkistrodon decoction

agkistrodon powder

and agkistrodon wine were administered daily starting from the onset of arthritis. Paw swelling degree was measured by using a volume-measuring instrument every 7 days after primary immunization. Arthritis index was measured and calculated using the “five scoring method” every 7 days. The levels of serum interleukin-1ß (IL-1ß) and type II collagen IgG antibodies were detected by enzyme-linked immunosorbent assay. Finally

all ankles were removed

and X-ray radiography was performed with In-vivo Imaging System FX. Samples were counterstained with hematoxylin and eosin for analysis. Among the various dosage formulations of agkistrodon

high-dose powder

which was equivalent to an amount of 6 g/day in adults

showed better effects on the inhibition of joint swelling and reduction of arthritis index score. The relatively low levels of serum IL-1 and anti-type II collagen IgG antibodies

as well as the X-ray radiography and pathology results

further proved the superiority of the high-dose powder over the other formulations. The effect of decoction on inhibiting joint swelling was inversely proportional to the dosage. Other effects

such as reduction of arthritis index score and the levels of serum IL-1 and anti-type II collagen IgG antibodies

were directly proportional to the dosage. While the use of large dose agkistrodon wine led to negative effects. These data highlight the potential function of high-dose agkistrodon powder

which was equivalent to an amount of 6 g/day in adults. The powder can quickly relieve the symptoms of rheumatoid arthritis and prevent aggravation of disease

especially during the early period.

Abstract

agkistrodon powder

all ankles were removed

and X-ray radiography was performed with In-vivo Imaging System FX. Samples were counterstained with hematoxylin and eosin for analysis. Among the various dosage formulations of agkistrodon

high-dose powder

which was equivalent to an amount of 6 g/day in adults

showed better effects on the inhibition of joint swelling and reduction of arthritis index score. The relatively low levels of serum IL-1 and anti-type II collagen IgG antibodies

as well as the X-ray radiography and pathology results

further proved the superiority of the high-dose powder over the other formulations. The effect of decoction on inhibiting joint swelling was inversely proportional to the dosage. Other effects

such as reduction of arthritis index score and the levels of serum IL-1 and anti-type II collagen IgG antibodies

were directly proportional to the dosage. While the use of large dose agkistrodon wine led to negative effects. These data highlight the potential function of high-dose agkistrodon powder

which was equivalent to an amount of 6 g/day in adults. The powder can quickly relieve the symptoms of rheumatoid arthritis and prevent aggravation of disease

especially during the early period.

关键词

rheumatoid arthritisagkistrodoncollagen-induced arthritisdifferent dosage formsChinese Medicine

Keywords

rheumatoid arthritisagkistrodoncollagen-induced arthritisdifferent dosage formsChinese Medicine

references

Rheumatology Branch of Chinese Medical Association. Guide for diagnosis and treatment of rheumatoid arthritis. Chin J Rheum (Chin) 2012;14:265-270.

Gonzalez A, Kremers HM, Crowson CS, Nicola PJ, Davis JM, Therneau TM, et al. The widening mortality gap between rheumatoid arthritis patients and the general population. Arthritis Rheum 2007;56:3583–3587.

Guo YJ, Chen J, Xiong XG, Wu D, Zhu H. Effect of bizhongxiao decoction and its decomposed formulas on collagen-induced arthritis in rat synovial effects of IL-1 and TNF. China J Mod Med (Chin) 2012;22:6–10.

Edward FR, Michael C, Andrew K, and Linda KM. Collageninduced arthritis. Curr Protoc Immunol 1996;15:1–24.

Xu SY, Bian RL, Chen X, eds. Pharmacological experimental methodology. 3rd ed. Beijing: People’s Medical Publishing House; 2002:1510–1511.

Zhang J, ed. Modern pharmacology experiment methods. Beijing: Beijing Medical College and Beijing Union Medical College Joint Publishing House; 1998.

Luo X, Zuo X, Mo X, Zhou Y, Xiao X. Treatment with recombinant Hsp72 suppresses collagen-induced arthritis in mice. Inflammation 2011;34:432–439.

Zwerina J, Hayer S, Tohidast-Akrad M, Bergmeister H, Readlich K, Feige U, et al. Single and combined inhibition of tumor necrosis factor, interleukin-1, and RANKL pathways in tumor necrosis factor-α induced arthritis: effects on synovial inflammation, bone erosion, and cartilage destruction. Arthritis Rheum 2004;50:277–290.

Lee SJ, Nam WD, Na HJ, Cho YL, Ha KS, Hwang JY, et al. CT20126, a novel immunosuppressant, prevents collageninduced arthritis through the down-regulation of inflammatory gene expression by inhibiting NF-kappaB activation. Biochem Pharmacol 2008;76:79–90.

Charles AD, Anna S, Jos WM. van der Meer. Treating inflammation by blocking interleukin-1 in a broad spectrum of diseases. Nat Rev Drug Discov 2012;11:633–652.

Huang ZY, Tan ZM, Wang SW, Wang JH. The value of antitype II collagen antibodies levels in rheumatoid arthritis activity evaluation. Chin Clin Rehabil (Chin) 2005;27:24–26.

Ding YM, Fu H, Zhu JL, Fu DB. The impact of arthritis on quality of life and prevention countermeasures. Shanghai J Prev Med (Chin) 2000;12:489–490.

Sun YL, Chi JH, Wang Y, Yu W, Sun SH, Hou J. Pharmacodynamic indexes and test methods of rats rheumatoid arthritis. Drug Eval Res (Chin) 2012;35:136–139.

Trentham DE, Townes AS, Kang AH. Autoimmunity to type II collagen: an experimemntal model of arthritis. J Exp Med 1977;146:857–868.

Li YK, ed. The traditional Chinese medicine pharmacology experimental methodology. 2nd ed. Shanghai: Shanghai Science and Technology Press; 2006:51.

Liu J, Sun Y. How does Chinese medicine target cytokine imbalance in rheumatoid arthritis? Chin J Integr Med 2013;19:874–880.

Nanjundaiah SM, Astry B, Moudgil KD. Mediators of inflammation-induced bone damage in arthritis and their control by herbal products. Evid Based Complement Alternt Med 2013:2013;518094.

Wang HY. To talk about the formulation reform of Chinese patent medicines based on the statistics and analysis of them with same names and different formulations. Chin Arch Tradit Chin Med (Chin) 2011;2:396–398.

Lei X, ed; Shang ZJ (jijiao author). Leigong’s theory of drug processing. Anhui: Anhui Science and Technology Press; 1991:122–124.

Zhu JP. Application of insect drugs in rheumatologylearning from the experiences of teacher Liangchun Zhu. Shanghai J Tradit Chin Med (Chin) 2008;42:13–14.

Fan J, ed. Exploration of powder. Beijing: Beijing Chinese Medicine Press; 1995:39–41.

National Pharmacopoeia Committee. Pharmacopoeia of People’s Republic of China (2010). 1st ed. Beijing: Chinese Medical Science and Technology Press; 2010:349-350.

Sakazaki F, Ogino H, Arakawa T, Okuno t, Ueno H. Lowdose ethanol aggravates allergic dermatitis in mice. Alcohol 2014;48:501–508.

Zhang FC, Wang JL, Wang ZG. The application of wine agent in Sheng Ji Zong Lu. J Tradit Chin med (Chin) 2012;27:1496–1498.

Qin YT. The application of snake wine on joint pain. New Tradit Chin Med (Chin) 1984;10:41.

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