Huaiqihuang Granules (槐杞黄颗粒) reduce proteinuria by enhancing nephrin expression and regulating necrosis factor κB signaling pathway in adriamycin-induced nephropathy

Hong Liu; Wei Sun; Liu-bao Gu; Yue Tu; Bing-yin Yu; Hao Hu

doi:10.1007/s11655-015-2293-0

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Huaiqihuang Granules (槐杞黄颗粒) reduce proteinuria by enhancing nephrin expression and regulating necrosis factor κB signaling pathway in adriamycin-induced nephropathy

OriginalPaper | Updated：2021-08-27

- Huaiqihuang Granules (槐杞黄颗粒) reduce proteinuria by enhancing nephrin expression and regulating necrosis factor κB signaling pathway in adriamycin-induced nephropathy
- Huaiqihuang Granules (槐杞黄颗粒) reduce proteinuria by enhancing nephrin expression and regulating necrosis factor κB signaling pathway in adriamycin-induced nephropathy
- 中国结合医学杂志（英文版） 2017年23卷第4期页码：279-287
- Affiliations：
  
  1. Department of Nephrology, the Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing,China
  2. Graduate School, Nanjing University of Chinese Medicine,Nanjing,China
  3. Center for Diabetes Care, Education and Research, Jiangsu Province Institute of Geriatrics,Nanjing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81373607), the Priority Academic Program Development of Jiangsu Higher Education Institutions, and the Science and Technology Funding for Life and Health Care of Jiangsu Province (No. BL2012032)
- DOI：10.1007/s11655-015-2293-0
  中图分类号：
- 纸质出版日期：2017，
  
  网络出版日期：2015-10-10，
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Liu, H., Sun, W., Gu, Lb. et al. Huaiqihuang Granules (槐杞黄颗粒) reduce proteinuria by enhancing nephrin expression and regulating necrosis factor κB signaling pathway in adriamycin-induced nephropathy., Chin. J. Integr. Med. 23, 279–287 (2017). https://doi.org/10.1007/s11655-015-2293-0

Hong Liu, Wei Sun, Liu-bao Gu, et al. Huaiqihuang Granules (槐杞黄颗粒) reduce proteinuria by enhancing nephrin expression and regulating necrosis factor κB signaling pathway in adriamycin-induced nephropathy[J]. Chinese Journal of Integrative Medicine, 2017,23(4):279-287.
Liu, H., Sun, W., Gu, Lb. et al. Huaiqihuang Granules (槐杞黄颗粒) reduce proteinuria by enhancing nephrin expression and regulating necrosis factor κB signaling pathway in adriamycin-induced nephropathy., Chin. J. Integr. Med. 23, 279–287 (2017). https://doi.org/10.1007/s11655-015-2293-0 DOI：

Hong Liu, Wei Sun, Liu-bao Gu, et al. Huaiqihuang Granules (槐杞黄颗粒) reduce proteinuria by enhancing nephrin expression and regulating necrosis factor κB signaling pathway in adriamycin-induced nephropathy[J]. Chinese Journal of Integrative Medicine, 2017,23(4):279-287. DOI： 10.1007/s11655-015-2293-0.

摘要

To investigate the effects of Huaiqihuang Granules (槐杞黄颗粒

HQH)

a mixture of Chinese herbs including Trametes robiniophila Murr

Fructus Lycii and Polygonatum sibiricum

on adriamycininduced nephropathy (ADRN) in rats and its underlying mechanisms. Rats with ADRN were divided into four groups: the sham group

the model group (distilled water)

the low-dose HQH-treated (2 g/kg) group

and the high-dose HQH-treated (4 g/kg) group. Body weight and 24-h urinary protein (Upro) were checked every week. After 5-week intervention

at the end of the study

the rats were sacrificed and blood samples were collected for examination of biochemical parameters

including glomerular morphological makers

podocyte shape

cellular apoptosis

expressions of nephrin

inflammatory and apoptosis markers. HQH ameliorated the rat’s general status

proteinuria

renal morphological appearance and glomerulosclerosis. The decreased expression of nephrin in ADRN rats was increased by HQH

as well as the impaired podocyte foot process fusion. Cytosolic levels of p65 and inhibitor of nuclear factor κBα (IκBα) were decreased in ADRN rats

and recovered by the treatment of HQH. Consistently

the induced expression of tumor necrosis factor α (TNF-α)

phosphorylated nuclear factor κB p65 (p-NFκB p65) and IκBα in ADRN were markedly suppressed by HQH. In addition

induction of Bax

cleaved caspase-3 and cytochrome C in ADRN rats were suppressed by HQH

indicating the amelioration of apoptosis. HQH could ameliorate renal impairments in ADRN rats by increasing nephrin expression

inhibiting NF-κB signaling pathway via the down-regulation of p-NF-κB p65 and p-IκBα

and suppression of glomerular and tubular apoptosis.

Abstract

To investigate the effects of Huaiqihuang Granules (槐杞黄颗粒

HQH)

a mixture of Chinese herbs including Trametes robiniophila Murr

Fructus Lycii and Polygonatum sibiricum

on adriamycininduced nephropathy (ADRN) in rats and its underlying mechanisms. Rats with ADRN were divided into four groups: the sham group

the model group (distilled water)

the low-dose HQH-treated (2 g/kg) group

and the high-dose HQH-treated (4 g/kg) group. Body weight and 24-h urinary protein (Upro) were checked every week. After 5-week intervention

at the end of the study

the rats were sacrificed and blood samples were collected for examination of biochemical parameters

including glomerular morphological makers

podocyte shape

cellular apoptosis

expressions of nephrin

inflammatory and apoptosis markers. HQH ameliorated the rat’s general status

proteinuria

renal morphological appearance and glomerulosclerosis. The decreased expression of nephrin in ADRN rats was increased by HQH

as well as the impaired podocyte foot process fusion. Cytosolic levels of p65 and inhibitor of nuclear factor κBα (IκBα) were decreased in ADRN rats

and recovered by the treatment of HQH. Consistently

the induced expression of tumor necrosis factor α (TNF-α)

phosphorylated nuclear factor κB p65 (p-NFκB p65) and IκBα in ADRN were markedly suppressed by HQH. In addition

induction of Bax

cleaved caspase-3 and cytochrome C in ADRN rats were suppressed by HQH

indicating the amelioration of apoptosis. HQH could ameliorate renal impairments in ADRN rats by increasing nephrin expression

inhibiting NF-κB signaling pathway via the down-regulation of p-NF-κB p65 and p-IκBα

and suppression of glomerular and tubular apoptosis.

关键词

adriamycin-induced nephropathyHuaiqihuang Granulesnephrinnuclear transcription factor kappa BapoptosisChinese Medicine

Keywords

adriamycin-induced nephropathyHuaiqihuang Granulesnephrinnuclear transcription factor kappa BapoptosisChinese Medicine

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