Antioxidative effect of luteolin pretreatment on simulated ischemia/reperfusion injury in cardiomyocyte and perfused rat heart

Rui-qu Zhang; Dong-ye Li; Tong-da Xu; Sha-sha Zhu; Huan-jun Pan; Fang Fang; Xin Wu; Hong Sun

doi:10.1007/s11655-015-2296-x

Your Location：

Home >

Browse articles >

Antioxidative effect of luteolin pretreatment on simulated ischemia/reperfusion injury in cardiomyocyte and perfused rat heart

OriginalPaper | Updated：2021-08-27

- Antioxidative effect of luteolin pretreatment on simulated ischemia/reperfusion injury in cardiomyocyte and perfused rat heart
- Antioxidative effect of luteolin pretreatment on simulated ischemia/reperfusion injury in cardiomyocyte and perfused rat heart
- 中国结合医学杂志（英文版） 2017年23卷第7期页码：518-527
- Affiliations：
  
  1. Institute of Cardiovascular Disease Research, Xuzhou Medical College,Jiangsu Province,Xuzhou,China
  2. Department of Physiology, Xuzhou Medical College,Jiangsu Province,Xuzhou,China
- Author bio：
- Funds：
  
  Supported by the Academic Degrees Committee and the Department of Education of Jiangsu Province (No. CXLX11-0732)
- DOI：10.1007/s11655-015-2296-x
  中图分类号：
- 纸质出版日期：2017，
  
  网络出版日期：2016-3-8，
Scan for full text
Zhang, Rq., Li, Dy., Xu, Td. et al. Antioxidative effect of luteolin pretreatment on simulated ischemia/reperfusion injury in cardiomyocyte and perfused rat heart., Chin. J. Integr. Med. 23, 518–527 (2017). https://doi.org/10.1007/s11655-015-2296-x

Rui-qu Zhang, Dong-ye Li, Tong-da Xu, et al. Antioxidative effect of luteolin pretreatment on simulated ischemia/reperfusion injury in cardiomyocyte and perfused rat heart[J]. Chinese Journal of Integrative Medicine, 2017,23(7):518-527.
Zhang, Rq., Li, Dy., Xu, Td. et al. Antioxidative effect of luteolin pretreatment on simulated ischemia/reperfusion injury in cardiomyocyte and perfused rat heart., Chin. J. Integr. Med. 23, 518–527 (2017). https://doi.org/10.1007/s11655-015-2296-x DOI：

Rui-qu Zhang, Dong-ye Li, Tong-da Xu, et al. Antioxidative effect of luteolin pretreatment on simulated ischemia/reperfusion injury in cardiomyocyte and perfused rat heart[J]. Chinese Journal of Integrative Medicine, 2017,23(7):518-527. DOI： 10.1007/s11655-015-2296-x.

摘要

To investigate the antioxidative effect and mechanism of luteolin on rat cardiomyocytes and isolated hearts followed by simulated ischemia/reperfusion (SI/R) injury. The left ventricular cardiomyocytes and the isolated hearts from adult rats were subjected to SI/R injury. The experiment groups included control

SI/R

luteolin + SI/R (Lut + SI/R)

vitamin E (Vit E) + SI/R

and LY294002 + luteolin + SI/R (LY + Lut + SI/R) groups. Cell viability

shortening amplitude

lactate dehydrogenase (LDH) release

superoxide dismutase (SOD) activity

the production of reactive oxygen species (ROS) and malondialdehyde (MDA)

expression levels of Akt

phosphorylated Akt

NOX2 (gp91phox)

NOX2 mRNA

mitogen-activated protein kinase (p38 MAPK) and phosphorylated p38MAPK were all measured after 3-h simulated ischemia and 2-h simulated reperfusion procedure in cardiomyocytes. Vit E was used as a standard control. The contractile function of isolated hearts was further observed after they were subjected to 30-min global ischemia and 120-min reperfusion. Pretreatment with 8-μmol/L luteolin substantially increased cell viability and shortening amplitude

while reducing evidence of oxidative stress-induced damage in the cells. In addition

the expression of NOX2

NOX2 mRNA and phosphorylation of p38MAPK were all downregulated. Furthermore

pretreatment with 40-μmol/L luteolin improved the recovery of myocardial contractile function following SI/R-induced injury

and luteolin markedly increased phosphorylation of Akt. However

all of the above effects were partially inhibited by the phosphatidylinositol 3-kinase (PI3K) inhibitor

LY294002. Luteolin prevents SI/R-induced myocardial damage by reducing oxidative stress-induced injury in isolated rat hearts and cardiomyocytes

and the cardioprotection induced by luteolin was partially mediated by the PI3K/Akt pathway.

Abstract

SI/R

luteolin + SI/R (Lut + SI/R)

vitamin E (Vit E) + SI/R

and LY294002 + luteolin + SI/R (LY + Lut + SI/R) groups. Cell viability

shortening amplitude

lactate dehydrogenase (LDH) release

superoxide dismutase (SOD) activity

the production of reactive oxygen species (ROS) and malondialdehyde (MDA)

expression levels of Akt

phosphorylated Akt

NOX2 (gp91phox)

NOX2 mRNA

while reducing evidence of oxidative stress-induced damage in the cells. In addition

the expression of NOX2

NOX2 mRNA and phosphorylation of p38MAPK were all downregulated. Furthermore

pretreatment with 40-μmol/L luteolin improved the recovery of myocardial contractile function following SI/R-induced injury

and luteolin markedly increased phosphorylation of Akt. However

all of the above effects were partially inhibited by the phosphatidylinositol 3-kinase (PI3K) inhibitor

LY294002. Luteolin prevents SI/R-induced myocardial damage by reducing oxidative stress-induced injury in isolated rat hearts and cardiomyocytes

and the cardioprotection induced by luteolin was partially mediated by the PI3K/Akt pathway.

关键词

luteolincardiomyocyteischemia/reperfusioncardioprotection

Keywords

luteolincardiomyocyteischemia/reperfusioncardioprotection

references

Dallas TX, ed. American Heart Association. The American Heart Association biostatistical fact sheets; 1997:1–29.

Kim JK, Pedram A, Razandi M, Levin ER. Estrogen prevents cardiomyocyte apoptosis through inhibition of reactive oxygen species and differential regulation of p38 kinase isoforms. J Biol Chem 2006;281:6760–6767.

Karyn L, ed. Antioxidants and Cardioprotection. Med Sci Sports Exerc 2007;1545–1553.

Mockridge JW, Marber MS, Heads RJ. Activation of Akt during simulated ischemia/reperfusion in cardiac myocytes. Biochem Biophys Res Commun 2000;270:947–952.

Fang F, Li DY, Pan HJ, Chen D, Qi LL, Zhang RQ, et al. Luteolin inhibits apoptosis and improves cardiomyocytes contractile function through PI3K/Akt pathway in simulated ischemia/reperfusion. Pharmacology 2011;88:149–158.

Saraste A, Pulkki K, Kallajoki M, Henriksen K, Parvinen M, Voipio-Pulkki LM. Apoptosis in human acute myocardial infarction. Circulation 1997;95:320–323.

Vanden Hoek TL, Qin Y, Wojcik K, Li CQ, Shao ZH, Anderson T, et al. Reperfusion, not simulated ischemia, initiates intrinsic apoptosis injury in chick cardiomyocytes. Am J Physiol 2003;284:H141–H150.

Lv XX, Yu XH, Wang HD, Yan YX, Wang YP, Lu DX, et al. Berberine inhibits norepinephrine-induced apoptosis in neonatal rat cardiomyocytes via inhibiting ROS-TNF-alpha-caspase signaling pathway. Chin J Integr Med 2012;19:424–431.

Zhao, ZQ. Oxidative stress-elicited myocardial apoptosis during reperfusion. Curr Opin Pharmacol 2004;4:159–165.

Sauer H, Wartenberg M, Hescheler J. Reactive oxygen species as intracellular messengers during cell growth and differentiation. Cell Physiol Biochem 2001;11:173–186.

Griendling KK, Sorescu D, Ushio-Fukai M. NAD(P)H oxidase: role in cardiovascular biology and disease. Circ Res 2000;86:494–501.

Anrather J, Racchumi G, Iadecola C. NF-kappaB regulates phagocytic NADPH oxidase by inducing the expression of gp91phox. J Biol Chem 2006;281:5657–5667.

Fu JH, Zheng YQ, LI P, Li XZ, Shang XH, Liu JX. Hawthorn leaves flavonoids decreases inflammation related to acute myocardial ischemia/reperfusion in anesthetized dogs. Chin J Integr Med 2013;19:582–588.

Li L, Henry GE, Seeram NP. Identification and bioactivities of resveratrol oligomers and flavonoids from Carex folliculata seeds. J Agr Food Chem 2009;57:7282–7287.

Chang J, Hsu Y, Kuo P, Kuo Y, Chiang L, Lin C. Increase of Bax/Bcl-XL ratio and arrest of cell cycle by luteolin in immortalized human hepatoma cell line. Life Sci 2005;76:1883–1893.

Li YC, Hung CF, Yeh FT, Lin JP, Chung JG. Luteolininhibited arylamine N-acetyltransferase activity and DNA-2-aminofluorene adduct in human and mouse leukemia cells. Food Chem Toxicol 2001;39:641–647.

Yee SB, Lee JH, Chung HY, Im KS, Bae SJ, Choi JS, et al. Inhibitory effects of luteolin isolated from Ixeris sonchifolia Hance on the proliferation of HepG2 human hepatocellular carcinoma cells. Arch Pharm Res 2003;26:151–156.

Wu MJ, Weng CY, Ding HY, Wu PJ. Anti-inflammatory and antiviral effects of Glossogyne tenuifolia. Life Sci 2005;76:1135–1146.

Choi CW, Jung HA, Kang SS, Choi JS. Antioxidant constituents and a new triterpenoid glycoside from Flos lonicerae. Arch Pharm Res 2007;30:1–7.

Hao Y, Sun Y, Xu C, Jiang X, Sun H, Wu Q, et al. Improvement of contractile function in isolated cardiomyocytes from ischemia-reperfusion rats by ginkgolide B pretreatment. J Cardiovasc Pharmacol 2009;54:3–9.

Zhao Z, Sun H, Yan CD, Gu SL, Wu Q. Oestrogen changed cardiomyocyte contraction and b2-adrenoceptor expression in rat hearts subjected to ischemia reperfusion. Exp Physiol 2008;93:1034–1043.

Sun H, Zhou F, Wang Y, Zhang Y, Chang A, Chen Q. Effects of beta-adrenoceptorsoverexpression on cell survival are mediated by Bax/Bcl-2 pathway in rat cardiac myocytes. Pharmacology 2006;78:98–104.

Qi LL, Pan HJ, Li DY, Fang F, Chen D, Sun H. Luteolin improves contractile function and attenuates apoptosis following ischemia-reperfusion in adult rat cardiomyocytes. Eur J Pharmacol 2011;668:201–207.

Das A, Smolenski A, Lohmann SM, Kukreja RC. Cyclic GMP-dependent protein kinase Ialpha attenuates necrosis and apoptosis following ischemia/reoxygenation in adult cardiomyocyte. J BiolChem 2006;281:38644–38652.

Denizot F, Lang R. Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Methods 1986;89:271–277.

Xu C, Liu A, Sun H, Sun Y, Wang G, Gao L, et al. Beta2-adrenoceptor confers cardioprotection against hypoxia in isolated ventricular myocytes and the effects depend on estrogenic environment. J Recept Signal Transduct Res 2010;30:255–261.

Gong H, Adamson DL, Ranu HK, Koch WJ, Heubach JF, Ravens U, et al. The effect of Gi-protein inactivation on basal, and β1-and β2AR-stimulated contraction of myocytes from transgenic mice overexpressing the β2-adrenoceptor. Br J Pharmacol 2000;131:594–600.

Zhou JJ, Pei JM, Wang GY, Wu S, Wang WP, Cho CH, et al. Inducible HSP70 mediates delayed cardioprotection via U-50488H pretreatment in rat ventricular myocytes. Am J Physiol Heart Circ Physiol 2001;28:H40–H47.

Liu D, He H, Li GL, Chen J, Yin D, Liao ZP, et al. Mechanisms of chloride in cardiomyocyte anoxiareoxygenation injury: the involvement of oxidative stress and NF-kappaB activation. Mol Cell Biochem 2011;355:201–209.

Bass DA, Parce JW, Dechatelet LR. Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation. J Immunol 1983;130:1910–1917.

Morrison LE, Whittaker RJ, Klepper RE, Wawrousek EF, Glembotski CC. Roles for alphaB-crystallin and HSPB2 in protecting the myocardium from ischemia-reperfusion induced damage in a KO mouse model. Am J Physiol. Heart Circ Physiol 2004;286:H847–H855.

Yu J, Zhang HF, Wu F, Li QX, Ma H, Guo WY, et al. Insulin improves cardiomyocyte contractile function through enhancement of SERCA2a activity in simulated ischemia/reperfusion1. Acta Pharmacol Sin 2006;27:919–926.

Horvathova K, Chalupa I, Sebova L, Tothova D, Vachalkova A. Protective effect of quercetin and luteolin in human melanomaHMB-2 cells. Mutat Res 2005;565:105–112.

Huang SS, Liu SM, Lin SM, Liao PH, Lin RH, Chen YC, et al. Antiarrhythmic effect of caffeic acid phenethyl ester (CAPE) on myocardial ischemia/reperfusion injury in rats. Clin Biochem 2005;38:943 947.

Tsutsui H, Kinugawa S, Matsushima S. Oxidative stress and mitochondrial DNA damage in heart failure. Circ J 2008;72:A31–A37.

Liao PH, Hung LM, Chen YH, Kuan YH, Zhang FB, Lin RH, et al. Cardioprotective effects of luteolin during ischemiareperfusion injury in rats. Circ J 2011;75:443–450.

Miura T, Miki T. GSK-3β, a therapeutic target for cardiomyocyte protection. Circ J 2009;73:1184–1192.

Hausenloy DJ, Tsang A, Mocanu MM, Yellon DM. Ischemic preconditioning protects by activating prosurvival kinases at reperfusion. Am J Physiol 2005;288:H971–H976.

FULL TEXT LINK

More>

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Dingxin Recipe (定心方) prevents ischemia/reperfusion-induced arrhythmias via up-regulating prohibitin and suppressing inflammatory responses

Extract of Paris polyphylla Simth protects cardiomyocytes from anoxia-reoxia injury through inhibition of calcium overload

Protection of ultra-filtration extract from Danggui Buxue Decoction (当归补血汤) on oxidative damage in cardiomyocytes of neonatal rats and its mechanism

Effects of serum containing Xinlikang on angiotensin II induced hypertrophy in cultured neonatal rat cardiomyocytes

Evodiamine Inhibits Angiotensin II-Induced Rat Cardiomyocyte Hypertrophy