Effects of hydro-alcoholic extract of Launaea acanthodes on serum gonadotropin and testosterone levels and the structure of seminiferous tubules in hyperglycemic rats

Ameneh Mohammadi; Morteza Behnam-Rassouli; Zeinab Momeni; Naser Mahdavi-Shahri

doi:10.1007/s11655-015-2315-y

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Effects of hydro-alcoholic extract of Launaea acanthodes on serum gonadotropin and testosterone levels and the structure of seminiferous tubules in hyperglycemic rats

OriginalPaper | Updated：2021-08-27

- Effects of hydro-alcoholic extract of Launaea acanthodes on serum gonadotropin and testosterone levels and the structure of seminiferous tubules in hyperglycemic rats
- Effects of hydro-alcoholic extract of Launaea acanthodes on serum gonadotropin and testosterone levels and the structure of seminiferous tubules in hyperglycemic rats
- 中国结合医学杂志（英文版） 2016年22卷第3期页码：207-213
- Affiliations：
  
  Department of Biology, Faculty of Science, Fredowsi University of Mashhad, Mashhad,Iran
- Author bio：
- Funds：
  
  Supported by the Grant from the Office of Research Affairs of Ferdowsi University of Mashhad, Mashhad, Iran (No. 3/17085)
- DOI：10.1007/s11655-015-2315-y
  中图分类号：
- 纸质出版日期：2016，
  
  网络出版日期：2015-12-17，
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Mohammadi, A., Behnam-Rassouli, M., Momeni, Z. et al. Effects of hydro-alcoholic extract of Launaea acanthodes on serum gonadotropin and testosterone levels and the structure of seminiferous tubules in hyperglycemic rats., Chin. J. Integr. Med. 22, 207–213 (2016). https://doi.org/10.1007/s11655-015-2315-y

Ameneh Mohammadi, Morteza Behnam-Rassouli, Zeinab Momeni, et al. Effects of hydro-alcoholic extract of Launaea acanthodes on serum gonadotropin and testosterone levels and the structure of seminiferous tubules in hyperglycemic rats[J]. Chinese Journal of Integrative Medicine, 2016,22(3):207-213.
Mohammadi, A., Behnam-Rassouli, M., Momeni, Z. et al. Effects of hydro-alcoholic extract of Launaea acanthodes on serum gonadotropin and testosterone levels and the structure of seminiferous tubules in hyperglycemic rats., Chin. J. Integr. Med. 22, 207–213 (2016). https://doi.org/10.1007/s11655-015-2315-y DOI：

Ameneh Mohammadi, Morteza Behnam-Rassouli, Zeinab Momeni, et al. Effects of hydro-alcoholic extract of Launaea acanthodes on serum gonadotropin and testosterone levels and the structure of seminiferous tubules in hyperglycemic rats[J]. Chinese Journal of Integrative Medicine, 2016,22(3):207-213. DOI： 10.1007/s11655-015-2315-y.

摘要

To investigate the effects of hydro-alcoholic extract of Launaea acanthodes

a blood glucose lowering plant in folk medicine of Iran

on the structure of seminiferous tubules and serum gonadotropin and testosterone levels in hyperglycemic rats. Twenty-four Wistar rats were randomly allocated into 4 groups (n=6): control

streptozotocin (STZ)

STZ + insulin [STZ + Ins

5 IU/(kg•day)]

and STZ + Launaea acanthodes extract [STZ + Ext

150 mg/(kg•day)]. Blood samples were collected at the 2nd and 4th weeks for detection of testosterone

follicle stimulating hormone (FSH) and luteinizing hormone (LH) with enzyme-linked immuno sorbent assay (ELISA)

and the right testes of rats were removed at the 7th week for the evaluation of diameter and wall thickness of seminiferous tubules and number of Leydig cells using unbiased stereological techniques. In comparison with the control group

at the 2nd week FSH (0.45 vs 0.03

0.02

0.02 IU/L in STZ

STZ + Ins and STZ + Ext groups

respectively) and LH (1.02 vs 0.37

0.2

0.29 IU/L) showed significant decreases (all P<0.05) and testosterone (4.2 vs 8.37

7.78

11.8 ng/mL) showed a remarkable increase (all P<0.05). The levels of these hormones became closer in the STZ + Ext and the STZ + Ins groups to the control at the 4th week. A significant decrease in diameter and wall thickness of seminiferous tubules and number of Leydig cells were observed in the STZ group as compared with the control (P<0.01). Administration of Launaea extract demonstrated a beneficial impact on the protection of testis from pathogenic and degenerative effects of hyperglycemia which may be partly due to its potential antioxidative effects.

Abstract

To investigate the effects of hydro-alcoholic extract of Launaea acanthodes

a blood glucose lowering plant in folk medicine of Iran

on the structure of seminiferous tubules and serum gonadotropin and testosterone levels in hyperglycemic rats. Twenty-four Wistar rats were randomly allocated into 4 groups (n=6): control

streptozotocin (STZ)

STZ + insulin [STZ + Ins

5 IU/(kg•day)]

and STZ + Launaea acanthodes extract [STZ + Ext

150 mg/(kg•day)]. Blood samples were collected at the 2nd and 4th weeks for detection of testosterone

follicle stimulating hormone (FSH) and luteinizing hormone (LH) with enzyme-linked immuno sorbent assay (ELISA)

at the 2nd week FSH (0.45 vs 0.03

0.02

0.02 IU/L in STZ

STZ + Ins and STZ + Ext groups

respectively) and LH (1.02 vs 0.37

0.2

0.29 IU/L) showed significant decreases (all P<0.05) and testosterone (4.2 vs 8.37

7.78

关键词

hyperglycemiaLaunaea acanthodestestisgonadotropinsTestosteronerat

Keywords

hyperglycemiaLaunaea acanthodestestisgonadotropinsTestosteronerat

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