Pretreatment with baicalin attenuates hypoxia and glucose deprivation-induced injury in SH-SY5Y cells

Qing-bo Zhou; Xiao-ning Ju; Xiao-yun Wang; Mei-hong Wang; Feng Kong; Chao Sun; Jian-zhong Bi

doi:10.1007/s11655-015-2326-8

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Pretreatment with baicalin attenuates hypoxia and glucose deprivation-induced injury in SH-SY5Y cells

OriginalPaper | Updated：2021-08-27

- Pretreatment with baicalin attenuates hypoxia and glucose deprivation-induced injury in SH-SY5Y cells
- Pretreatment with baicalin attenuates hypoxia and glucose deprivation-induced injury in SH-SY5Y cells
- 中国结合医学杂志（英文版） 2016年22卷第3期页码：201-206
- Affiliations：
  
  1. Department of Neurology, the Second Hospital of Shandong University,Jinan,China
  2. Central Laboratory, the Second Hospital of Shandong University,Jinan,China
- Author bio：
- Funds：
  
  Supported by Seed Fund of the 2nd Hospital of Shandong University (No. S2013010002).
- DOI：10.1007/s11655-015-2326-8
  中图分类号：
- 纸质出版日期：2016，
  
  网络出版日期：2015-12-19，
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Zhou, Qb., Ju, Xn., Wang, Xy. et al. Pretreatment with baicalin attenuates hypoxia and glucose deprivation-induced injury in SH-SY5Y cells., Chin. J. Integr. Med. 22, 201–206 (2016). https://doi.org/10.1007/s11655-015-2326-8

Qing-bo Zhou, Xiao-ning Ju, Xiao-yun Wang, et al. Pretreatment with baicalin attenuates hypoxia and glucose deprivation-induced injury in SH-SY5Y cells[J]. Chinese Journal of Integrative Medicine, 2016,22(3):201-206.
Zhou, Qb., Ju, Xn., Wang, Xy. et al. Pretreatment with baicalin attenuates hypoxia and glucose deprivation-induced injury in SH-SY5Y cells., Chin. J. Integr. Med. 22, 201–206 (2016). https://doi.org/10.1007/s11655-015-2326-8 DOI：

Qing-bo Zhou, Xiao-ning Ju, Xiao-yun Wang, et al. Pretreatment with baicalin attenuates hypoxia and glucose deprivation-induced injury in SH-SY5Y cells[J]. Chinese Journal of Integrative Medicine, 2016,22(3):201-206. DOI： 10.1007/s11655-015-2326-8.

摘要

To explore the neuroprotective effects of baicalin against hypoxia and glucose deprivationreperfusion (OGD/RO)-induced injury in SH-SY5Y cells. SH-SY5Y cells were divided into a control group

a OGD/RO group

which was subject to OGD/RO induction; and 3 baicalin groups subject to baicalin (1

25 μmol/L) for 2 h before induction of OGD/RO (low-

medium-

and high-dose baicalin groups). Cell viability was detected by thiazolyl blue tetrazolium bromide (MTT) assay and flow cytometric analysis was used to detect cell apoptosis. Real-time polymerase chain reaction was performed to determine the mRNA expression of caspase-3 gene. Western blot analysis was conducted to determine the expression of nuclear factor (NF)-κB and N-methyl-daspartic acid receptor-1 (NMDAR1). Baicalin could significantly attenuate OGD/RO mediated apoptotic cell death in SH-SY5Y cells; the apoptosis rates in the low-

medium- and high-dose groups were 12.1%

7.9%

and 5.4%

respectively. Western blot and real-time PCR analysis revealed that significant decrease in caspase-3 expression in the baicalin group compared with the OGD/RO group (P<0.01). Additionally

down-regulation of NF-κB and NMDAR1 was observed in the baicalin group compared with those obtained from the OGD/RO group. Compared with the low-dose baicalin group

remarkable decrease was noted in the medium- and high-dose groups (P<0.01). Baicalin pre-treatment attenuates brain ischemia reperfusion injury by suppressing cellular apoptosis.

Abstract

To explore the neuroprotective effects of baicalin against hypoxia and glucose deprivationreperfusion (OGD/RO)-induced injury in SH-SY5Y cells. SH-SY5Y cells were divided into a control group

a OGD/RO group

which was subject to OGD/RO induction; and 3 baicalin groups subject to baicalin (1

25 μmol/L) for 2 h before induction of OGD/RO (low-

medium-

medium- and high-dose groups were 12.1%

7.9%

and 5.4%

respectively. Western blot and real-time PCR analysis revealed that significant decrease in caspase-3 expression in the baicalin group compared with the OGD/RO group (P<0.01). Additionally

down-regulation of NF-κB and NMDAR1 was observed in the baicalin group compared with those obtained from the OGD/RO group. Compared with the low-dose baicalin group

remarkable decrease was noted in the medium- and high-dose groups (P<0.01). Baicalin pre-treatment attenuates brain ischemia reperfusion injury by suppressing cellular apoptosis.

关键词

Baicalinneuroprotectionapoptosiscaspase-3nuclear factor-κBN-methyl-d-aspartic acid receptor-1hypoxia and glucose deptivation-reperfusion

Keywords

Baicalinneuroprotectionapoptosiscaspase-3nuclear factor-κBN-methyl-d-aspartic acid receptor-1hypoxia and glucose deptivation-reperfusion

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