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Effect of Bushen Yisui Capsule (补肾益髓胶囊) on oligodendrocyte lineage genes 1 and 2 in mice with experimental autoimmune encephalomyelitis
Effect of Bushen Yisui Capsule (补肾益髓胶囊) on oligodendrocyte lineage genes 1 and 2 in mice with experimental autoimmune encephalomyelitis
- 中国结合医学杂志（英文版） 2016年22卷第12期 页码：932-940
- Affiliations：
1. Department of Traditional Chinese Medicine, Beijing Tiantan Hospital, Capital Medical University,Beijing,China
2. School of Traditional Chinese Medicine, Capital Medical University,Beijing,China
3. Department of Oncology, Guang’anmen Hospital of China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
DOI：10.1007/s11655-015-2431-8
中图分类号：纸质出版日期：2016，
网络出版日期：2016-2-26，
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Yang, T., Zheng, Q., Zhao, H. et al. Effect of Bushen Yisui Capsule (补肾益髓胶囊) on oligodendrocyte lineage genes 1 and 2 in mice with experimental autoimmune encephalomyelitis., Chin. J. Integr. Med. 22, 932–940 (2016). https://doi.org/10.1007/s11655-015-2431-8
Tao Yang, Qi Zheng, Hui Zhao, et al. Effect of Bushen Yisui Capsule (补肾益髓胶囊) on oligodendrocyte lineage genes 1 and 2 in mice with experimental autoimmune encephalomyelitis[J]. Chinese Journal of Integrative Medicine, 2016,22(12):932-940.
Yang, T., Zheng, Q., Zhao, H. et al. Effect of Bushen Yisui Capsule (补肾益髓胶囊) on oligodendrocyte lineage genes 1 and 2 in mice with experimental autoimmune encephalomyelitis., Chin. J. Integr. Med. 22, 932–940 (2016). https://doi.org/10.1007/s11655-015-2431-8 DOI：
Tao Yang, Qi Zheng, Hui Zhao, et al. Effect of Bushen Yisui Capsule (补肾益髓胶囊) on oligodendrocyte lineage genes 1 and 2 in mice with experimental autoimmune encephalomyelitis[J]. Chinese Journal of Integrative Medicine, 2016,22(12):932-940. DOI： 10.1007/s11655-015-2431-8.
摘要
To study the effects of Bushen Yisui Capsule (补肾益髓胶囊
BSYSC) on the oligodendrocyte lineage genes (Olig) 1 and Olig2 in C57BL/6 mice with experimental autoimmune encephalomyelitis (EAE) in order to explore the remyelination effect of BSYSC. The mice were randomly divided into normal control (NC)
EAE model (EAE-M)
prednisone acetate (PA
6 mg/kg)
BSYSC high-dose (3.02 g/kg) and BSYSC low-dose (1.51 g/kg) groups. The mice were induced by immunization with myelin oligodendrocyte glycoprotein (MOG) 35-55. The neurological function scores were assessed once daily. The pathological changes in mice brains were observed with hematoxylin-eosin (HE) staining and transmission electron microscope (TEM). The protein expressions of myelin basic protein (MBP)
Olig1 and Olig2 in brains were measured by immunohistochemistry. The mRNA expressions of Olig1 and Olig 2 was also determined by quantitative real-time polymerase chain reaction. Compared with the EAE-M mice
(1) the neurological function scores were significantly decreased in BSYSC-treated mice on days 22 to 40 (P<0.01); (2) the inflammatory cells and demyelination in brains were reduced in BSYSC-treated EAE mice; (3) the protein expression of MBP was markedly increased in BSYSC-treated groups on day 18 and 40 respectively (P<0.05 or P<0.01); (4) the protein expression of Olig1 was increased in BSYSC (3.02 g/kg)-treated EAE mice on day 40 (P<0.01). Protein and mRNA expression of Olig2 was increased in BSYSC-treated EAE mice on day 18 and 40 (P<0.01). The effects of BSYSC on reducing demyelination and promoting remyelination might be associated with the increase of Olig1 and Olig2.
Abstract
To study the effects of Bushen Yisui Capsule (补肾益髓胶囊
BSYSC) on the oligodendrocyte lineage genes (Olig) 1 and Olig2 in C57BL/6 mice with experimental autoimmune encephalomyelitis (EAE) in order to explore the remyelination effect of BSYSC. The mice were randomly divided into normal control (NC)
EAE model (EAE-M)
prednisone acetate (PA
6 mg/kg)
BSYSC high-dose (3.02 g/kg) and BSYSC low-dose (1.51 g/kg) groups. The mice were induced by immunization with myelin oligodendrocyte glycoprotein (MOG) 35-55. The neurological function scores were assessed once daily. The pathological changes in mice brains were observed with hematoxylin-eosin (HE) staining and transmission electron microscope (TEM). The protein expressions of myelin basic protein (MBP)
Olig1 and Olig2 in brains were measured by immunohistochemistry. The mRNA expressions of Olig1 and Olig 2 was also determined by quantitative real-time polymerase chain reaction. Compared with the EAE-M mice
(1) the neurological function scores were significantly decreased in BSYSC-treated mice on days 22 to 40 (P<0.01); (2) the inflammatory cells and demyelination in brains were reduced in BSYSC-treated EAE mice; (3) the protein expression of MBP was markedly increased in BSYSC-treated groups on day 18 and 40 respectively (P<0.05 or P<0.01); (4) the protein expression of Olig1 was increased in BSYSC (3.02 g/kg)-treated EAE mice on day 40 (P<0.01). Protein and mRNA expression of Olig2 was increased in BSYSC-treated EAE mice on day 18 and 40 (P<0.01). The effects of BSYSC on reducing demyelination and promoting remyelination might be associated with the increase of Olig1 and Olig2.
关键词
experimental autoimmune encephalomyelitisBushen Yisui Capsulemyelin basic proteinoligodendrocyte lineage gene 1oligodendrocyte lineage gene 2Chinese Medicine
Keywords
experimental autoimmune encephalomyelitisBushen Yisui Capsulemyelin basic proteinoligodendrocyte lineage gene 1oligodendrocyte lineage gene 2Chinese Medicine
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