Tonglian Decoction (通莲汤) arrests the cell cycle in S-phase by targeting the nuclear factor-kappa B signal pathway in esophageal carcinoma Eca109 cells

Yong-sen Jia; Xue-qin Hu; Ji-an Li; Szasz Andras; Gabriella Hegyi; Bing-sheng Han

doi:10.1007/s11655-016-2096-3

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Tonglian Decoction (通莲汤) arrests the cell cycle in S-phase by targeting the nuclear factor-kappa B signal pathway in esophageal carcinoma Eca109 cells

OriginalPaper | Updated：2021-08-27

- Tonglian Decoction (通莲汤) arrests the cell cycle in S-phase by targeting the nuclear factor-kappa B signal pathway in esophageal carcinoma Eca109 cells
- Tonglian Decoction (通莲汤) arrests the cell cycle in S-phase by targeting the nuclear factor-kappa B signal pathway in esophageal carcinoma Eca109 cells
- 中国结合医学杂志（英文版） 2016年22卷第5期页码：384-389
- Affiliations：
  
  1. Basic Section, College of Chinese Medicine, North China University of Science and Technology,Hebei Province,Tangshan,China
  2. Institute of Orthopaedics and Traumatology, Zhejiang Chinese Medical University,Hangzhou,China
  3. Oncotherm Innovation and Trade Ltd., Pecs,Hungary
  4. Acupuncture Rehabilitation Foundation,Budapest,Hungary
  5. Traditional Chinese Medicine Hospital of Shenzhen,Guangdong Province,Shenzhen,China
- Author bio：
- Funds：
  
  Supported by the Natural Science Fundation of Hebei Province of China (No. H2013209053), Scientific and Technological Project of Administration of Traditional Chinese Medicine of Hebei Province, China (No. 2014185)
- DOI：10.1007/s11655-016-2096-3
  中图分类号：
- 纸质出版日期：2016，
  
  网络出版日期：2016-3-5，
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Jia, Ys., Hu, Xq., Li, Ja. et al. Tonglian Decoction (通莲汤) arrests the cell cycle in S-phase by targeting the nuclear factor-kappa B signal pathway in esophageal carcinoma Eca109 cells., Chin. J. Integr. Med. 22, 384–389 (2016). https://doi.org/10.1007/s11655-016-2096-3

Yong-sen Jia, Xue-qin Hu, Ji-an Li, et al. Tonglian Decoction (通莲汤) arrests the cell cycle in S-phase by targeting the nuclear factor-kappa B signal pathway in esophageal carcinoma Eca109 cells[J]. Chinese Journal of Integrative Medicine, 2016,22(5):384-389.
Jia, Ys., Hu, Xq., Li, Ja. et al. Tonglian Decoction (通莲汤) arrests the cell cycle in S-phase by targeting the nuclear factor-kappa B signal pathway in esophageal carcinoma Eca109 cells., Chin. J. Integr. Med. 22, 384–389 (2016). https://doi.org/10.1007/s11655-016-2096-3 DOI：

Yong-sen Jia, Xue-qin Hu, Ji-an Li, et al. Tonglian Decoction (通莲汤) arrests the cell cycle in S-phase by targeting the nuclear factor-kappa B signal pathway in esophageal carcinoma Eca109 cells[J]. Chinese Journal of Integrative Medicine, 2016,22(5):384-389. DOI： 10.1007/s11655-016-2096-3.

摘要

To investigate the anti-tumor activity and molecular mechanism of Tonglian Decoction (通莲汤

TLD) on esophageal carcinoma Eca109 cells. Eca109 cells were treated with TLD and its separated formulae

including the clearing-heat and detoxification formula (Q)

activating-blood and promoting-qi formula (H) and nourishing-yin and blood formula (Z). Cell proliferation was measured using the 3-(4

5-dimethyl-2-thiazolyl)-2

5-diphenyl-2-H-tetrazolium bromide assay

cell morphology was observed using a microscope

the cell cycle was measured using flow cytometry and the activity of the nuclear factor-kappa B (NF-κB) signal pathway was detected by Western blot. The half maximal inhibitory concentrations of TLD

Q and H were 386

771 and 729 mg/L

respectively. TLD

Q and H significantly inhibited cell proliferation

with 69.43%

60.84% and 61.90% of treated cells in the G phase of the cell cycle. The percentage of cells in S phase increased significantly after treatment with TLD

and H compared with the control group (P<0.05)

and TLD showed the strongest effect. Z had no influence on the cell cycle compared with the control group (P>0.05). Western blot detection indicated slight differences in the inhibition of the NF-κB pathway by the different formulae. TLD formula strongly inhibited IKKβ

NF-κB

interleukin-6 and tumor necrosis factor-α expression compared with the control group. TLD inhibited Eca109 cell proliferation by arresting cells in S phase. The possible mechanism might be related to inhibiting the NF-κB transduction cascade. The combination of the herbs found in the three separate formulae

Q and Z

work synergistically in TLD to produce the inhibitory effects of TLD treatment on Eca109 proliferation.

Abstract

To investigate the anti-tumor activity and molecular mechanism of Tonglian Decoction (通莲汤

TLD) on esophageal carcinoma Eca109 cells. Eca109 cells were treated with TLD and its separated formulae

including the clearing-heat and detoxification formula (Q)

activating-blood and promoting-qi formula (H) and nourishing-yin and blood formula (Z). Cell proliferation was measured using the 3-(4

5-dimethyl-2-thiazolyl)-2

5-diphenyl-2-H-tetrazolium bromide assay

cell morphology was observed using a microscope

the cell cycle was measured using flow cytometry and the activity of the nuclear factor-kappa B (NF-κB) signal pathway was detected by Western blot. The half maximal inhibitory concentrations of TLD

Q and H were 386

771 and 729 mg/L

respectively. TLD

Q and H significantly inhibited cell proliferation

with 69.43%

60.84% and 61.90% of treated cells in the G phase of the cell cycle. The percentage of cells in S phase increased significantly after treatment with TLD

and H compared with the control group (P<0.05)

NF-κB

Q and Z

work synergistically in TLD to produce the inhibitory effects of TLD treatment on Eca109 proliferation.

关键词

esophageal carcinomaEca109 cells Tonglian Decoctionproliferationcell cyclenuclear factor-kappa BChinese Medicine

Keywords

esophageal carcinomaEca109 cells Tonglian Decoctionproliferationcell cyclenuclear factor-kappa BChinese Medicine

references

Safaee A, Fatemi SR, Moghimi-Dehkordi B, Ghiasi S, Zali MR. Epidemiology and histopathological features of esophageal cancer. East Afr J Public Health 2012;9:7–9.

Gao QY, Fang JY. Early esophageal cancer screening in China. Best Pract Res Clin Gatroenterol 2015;29:885–893.

Zhong C, Tan JJ, Xu ZX. Progress on etiology and epidemiology of esophageal carcinoma. Henan J Prev Med (Chin) 2011;22:1–11.

Jia YS, Lv CT, Wu FW, Yang YY, Zhao S. Research on Tongyou Decoction and its separated formulae on signal pathway mediated by PI3K/AKT in esophageal carcinoma cell line EC9706. Liaoning J Tradit Chin Med (Chin) 2011;38:1306–1308.

Jia YS, Wang YY, Si FC. Regulation of prescriptions for esophageal carcinoma on growth signal transduction in hEGF-stimulated cell line EC9706. Chin J Exp Med Form (Chin) 2010;16(3):100–108.

Liu ZC, Jia YS, Bao JT. Research on Tongyou Decoction and its separated formulae on apoptosis signal pathway mediated by caspase-3 in esophageal squamous carcinoma cell. Jiangsu J Tradit Chin Med (Chin) 2011;43(7):86–88.

Faried A, Kurnia D, Faried LS, Usman N, Miyazaki T, Kato H, et al. Anticancer effects of gallic acid isolated from Indonesian herbal medicine, Phaleria macrocarpa (Scheff. ) Boerl, on human cancer cell lines. Int J Oncol 2007;30:605–613.

Wang L, Deng Y, Li T, Yang H. Quantitative assessment of right ventricular systolic function by the analysis of right ventricular contrast time-intensity curve. J Huazhong Univ Sci Technol Med Sci (Chin) 2004;24:607–609.

Anbalagan S, Biasoli D, Leszczynska KB, Mukherjee S, Hammond EM. In vitro radiosensitization of esophageal cancer cells with the aminopeptidase inhibitor CHR-2797. Radiat Res 2015;184:259–265.

Liu DB, Hu GY, Long GX, Qiu H, Mei Q, Hu GQ. Celecoxib induces apoptosis and cell-cycle arrest in nasopharyngeal carcinoma cell lines via inhibition of STAT3 phosphorylation. Acta Pharmacol Sin 2012;33:682–690.

Farney JK, Mamedova LK, Godsey BH, Bradford BJ. Technical note: validation of an ELISA for measurement of tumor necrosis factor alpha in bovine plasma. J Dairy Sci 2011;94:3504–3509.

Burnette WN. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem 1981;112:195–203.

Lizuka N, Hazama S, Yoshimura K, Yoshino S, Tangoku A, Miyamoto K, et al. Anticachectic effects of the natural herb Coptidis rhizoma and berberine on mice bearing colon 26/clone 20 adenocarcinoma. Int J Cancer 2002;99:286–291.

Hou J, Lin PZ, Chen ZF, Ding ZW, Li SS, Men FS, et al. Field population-based blocking treatment of esophageal epithelia dysplasia. World J Gastroenterol 2002;8:418–422.

Xian MS, Hayashi K, Lu JP, Awai M. Efficacy of traditional Chinese herbs on squamous cell carcinoma of the esophagus: histopathologic analysis of 240 cases. Acta Med Okayama 1989;43:345–351.

Li Y, Guo C, Wang Z, Gong P, Sun Z, Gong S. Enhanced effects of TRAIL-endostatin-based double-generadiotherapy on suppressing growth, promoting apoptosis and inducing cell cycle arrest in vascular endothelial cells. J Huazhong Univ Sci Technolog Med Sci (Chin) 2012;32:167–172.

Guo W, Zou YB, Jiang YG, Wang RW, Zhao YP, Ma Z. Zinc induces cell cycle arrest and apoptosis by upregulation of WIG-1 in esophageal squamous cancer cell line EC109. Tumour Biol 2011;32:801–808.

Ding DP, Chen ZL, Zhao XH, Wang JW, Sun J, Wang Z, et al. miR-29c induces cell cycle arrest in esophageal squamous cell carcinoma by modulating cyclin E expression. Carcinogenesis 2011;32:1025–1032.

Dibb M, Ang YS. Targeting the cell cycle in esophageal adenocarcinoma: an adjunct to anticancer treatment. World J Gastroenterol 2011;17:2063–2069.

Tian F, Xu PR, Hou WH. The activation of NF-κB signaling transduction pathway in esophageal squamous cell carcinoma cell lines. Cancer Res Prev Treat (Chin) 2006;33:11–14.

Li J, Wang K, Chen X, Meng H, Song M, Wang Y, et al. Transcriptional activation of microRNA-34a by NF-kappa B in human esophageal cancer cells. BMC Mol Biol 2012;13:4–13.

Su C, Chen Z, Luo H, Su Y, Liu W, Cai L, et al. Different patterns of NF-κB and Notch1 signaling contribute to tumor-induced lymphangiogenesis of esophageal squamous cell carcinoma. J Exp Clin Cancer Res 2011;30:85–93.

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