Molecular Pathway of Psoralidin-Induced Apoptosis in HepG2 Cell Line

Bin YU; An-hong WANG; Kun ZHOU; Li-juan CHAI; Lu LIU

doi:10.1007/s11655-016-2251-5

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Molecular Pathway of Psoralidin-Induced Apoptosis in HepG2 Cell Line

Original Article | Updated：2021-08-27

- Molecular Pathway of Psoralidin-Induced Apoptosis in HepG2 Cell Line
- Molecular Pathway of Psoralidin-Induced Apoptosis in HepG2 Cell Line
- Chinese Journal of Integrative Medicine 2019年25卷第10期页码：757-762
- Affiliations：
  
  1.School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin (300193), China
  2.Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin (300193), China
  3.Department of pharmacy, Gansu Provincial Hospital, Lanzhou (731600), China
  4.Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin (300193), China
  5.College of Pharmacy, Henan University, Kaifeng, Henan Province (475004), China
- Author bio：
  
  Correspondence to: Prof. ZHOU Kun, Tel: 86-22-59596164, Fax: 86-22-27386453, E-mail: z.k.ken@263.net
- Funds：
  
  the National Natural Science Foundation of China(81202991);Tianjin City Application Basis, Cutting-edge Technology Research Program (Tianjin Municipal Science and Technology Commission)(13JCYBJC38500)
- DOI：10.1007/s11655-016-2251-5
  中图分类号：
- 纸质出版日期：2019-10-01，
  
  网络出版日期：2016-03-29，
  
  录用日期：2015-01-09
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Bin YU, An-hong WANG, Kun ZHOU, 等. Molecular Pathway of Psoralidin-Induced Apoptosis in HepG2 Cell Line[J]. Chinese Journal of Integrative Medicine, 2019,25(10):757-762.

Bin YU, An-hong WANG, Kun ZHOU, et al. Molecular Pathway of Psoralidin-Induced Apoptosis in HepG2 Cell Line[J]. Chinese Journal of Integrative Medicine, 2019,25(10):757-762.
Bin YU, An-hong WANG, Kun ZHOU, 等. Molecular Pathway of Psoralidin-Induced Apoptosis in HepG2 Cell Line[J]. Chinese Journal of Integrative Medicine, 2019,25(10):757-762. DOI： 10.1007/s11655-016-2251-5.

Bin YU, An-hong WANG, Kun ZHOU, et al. Molecular Pathway of Psoralidin-Induced Apoptosis in HepG2 Cell Line[J]. Chinese Journal of Integrative Medicine, 2019,25(10):757-762. DOI： 10.1007/s11655-016-2251-5.

摘要

Abstract

Objective:

To test the role of psoralidin in human liver cancer HepG2 cells in vitro.

Methods:

Cell viability was assessed by methylthiazolyldiphenyl-tetrazolum bromide assay and apoptotic cells were labeled by annexin V then sorted by flow cytometry. Protein expressions of caspase-3

caspase-8

caspase-9

Bax

Bid

Bcl-2

Bcl-xL and p53 were examined by western blot while activity of caspase-3

-8 and -9 were also determined.

Results:

Psoralidin reduces cell viability greatly in a time dependent manner (64%

40%

21%

12% at 2

24 and 48 h treatment with 64 μmol/L psoralidin respectively) and up-regulates activities of caspase-3

-8 and -9 in a concentration dependent manner (between 4 to 64 μmol/L). Psoralidin also increases the expression of pro-apoptosis genes Bax

Bid and p53 while decreases the expression of pro-survival genes Bcl-2 and Bcl-xL

both in a concentration dependent manner between 4 and 64 μmol/L (

0.05 at 16 and 64 μmol/L). Caspase-3 inhibitor (Ac-DEVD-CHO at concentrations between 10 to 20 μmol/L)

p53 inhibitor (pifithrin-α at 5 μmol/L) and cyclosporin A can attenuate the apoptotic effect of psoralidin.

Conclusion:

The cytotoxic role of psoralidin might work through both intrinsic and extrinsic apoptotic pathway.

关键词

Keywords

psoralidinapoptosisHepG2mitochondriap53cyclosporin A

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