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Effect of Chinese herbal compound Naofucong (脑复聪) on the inflammatory process induced by high glucose in BV-2 cells
Effect of Chinese herbal compound Naofucong (脑复聪) on the inflammatory process induced by high glucose in BV-2 cells
- 中国结合医学杂志（英文版） 2016年22卷第11期 页码：832-839
- Affiliations：
1. Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences,Beijing,China
2. Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College,Beijing,China
- Author bio：
- Funds：Supported by Beijing Natural Science Foundation (No. 7142129), and Beijing Science and Technology Project of Traditional Chinese Medicine (No. JJ2014-50), China
DOI：10.1007/s11655-016-2256-0
中图分类号：纸质出版日期：2016，
网络出版日期：2016-5-25，
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Jing, Gc., Zhang, Mr., Ji, C. et al. Effect of Chinese herbal compound Naofucong (脑复聪) on the inflammatory process induced by high glucose in BV-2 cells., Chin. J. Integr. Med. 22, 832–839 (2016). https://doi.org/10.1007/s11655-016-2256-0
Guang-chan Jing, Meng-ren Zhang, Chao Ji, et al. Effect of Chinese herbal compound Naofucong (脑复聪) on the inflammatory process induced by high glucose in BV-2 cells[J]. Chinese Journal of Integrative Medicine, 2016,22(11):832-839.
Jing, Gc., Zhang, Mr., Ji, C. et al. Effect of Chinese herbal compound Naofucong (脑复聪) on the inflammatory process induced by high glucose in BV-2 cells., Chin. J. Integr. Med. 22, 832–839 (2016). https://doi.org/10.1007/s11655-016-2256-0 DOI：
Guang-chan Jing, Meng-ren Zhang, Chao Ji, et al. Effect of Chinese herbal compound Naofucong (脑复聪) on the inflammatory process induced by high glucose in BV-2 cells[J]. Chinese Journal of Integrative Medicine, 2016,22(11):832-839. DOI： 10.1007/s11655-016-2256-0.
摘要
To determine the effect of medicated serum of Chinese herbal compound Naofucong (脑复聪
NFC) on the microglia BV-2 cells viability and the transcription and expression of interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α) in microglia BV-2 cells to further explore the mechanisms underlying the protective effect of NFC on inflammatory process induced by high glucose. The microglia BV-2 cells incubated in vitro were divided into different groups: the control group (25 mmol/L glucose)
the model group (75 mmol/L glucose)
high glucose media containing different dose medicated serum of NFC. After being cultured for 24 h
changes in IL-6 and TNF-α were measured by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay. The expression of surface marker CD11b of activated microglia was measured by confocal laser scanning microscope and Western blot. Nuclear factor-κB (NF-κB) p-p65 expression was analyzed by Western blot. The model group obviously increased the expression of microglial surface marker CD11b and NF-κB p-p65 (all P<0.01)
induced a signifificant up-regulation of release and the mRNA expression of IL-6 and TNF-α (P<0.01 or P<0.05). The medicated serum of NFC could obviously down-regulate the transcription and expression of surface marker CD11 b and NF-κB p-p65 (all P<0.01)
and inhibit the mRNA and protein expression (P<0.01 or P<0.05) of inflflammatory cytokines
such as IL-6 and TNF-α
in microglia BV-2 cells cultured with high glucose for 24 h. The inhibition of microglial activation and IL-6 and TNF-α expression induced by high glucose may at least partly explain NFC therapeutic effects on diabetes-associated cognitive decline diseases. Its underlying mechanism could probably be related to the inhibition of NFC on NF-κB phosphorylation.
Abstract
To determine the effect of medicated serum of Chinese herbal compound Naofucong (脑复聪
NFC) on the microglia BV-2 cells viability and the transcription and expression of interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α) in microglia BV-2 cells to further explore the mechanisms underlying the protective effect of NFC on inflammatory process induced by high glucose. The microglia BV-2 cells incubated in vitro were divided into different groups: the control group (25 mmol/L glucose)
the model group (75 mmol/L glucose)
high glucose media containing different dose medicated serum of NFC. After being cultured for 24 h
changes in IL-6 and TNF-α were measured by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay. The expression of surface marker CD11b of activated microglia was measured by confocal laser scanning microscope and Western blot. Nuclear factor-κB (NF-κB) p-p65 expression was analyzed by Western blot. The model group obviously increased the expression of microglial surface marker CD11b and NF-κB p-p65 (all P<0.01)
induced a signifificant up-regulation of release and the mRNA expression of IL-6 and TNF-α (P<0.01 or P<0.05). The medicated serum of NFC could obviously down-regulate the transcription and expression of surface marker CD11 b and NF-κB p-p65 (all P<0.01)
and inhibit the mRNA and protein expression (P<0.01 or P<0.05) of inflflammatory cytokines
such as IL-6 and TNF-α
in microglia BV-2 cells cultured with high glucose for 24 h. The inhibition of microglial activation and IL-6 and TNF-α expression induced by high glucose may at least partly explain NFC therapeutic effects on diabetes-associated cognitive decline diseases. Its underlying mechanism could probably be related to the inhibition of NFC on NF-κB phosphorylation.
关键词
high glucosemicrogliainflflammatory cytokineChinese herbal compound Naofucong
Keywords
high glucosemicrogliainflflammatory cytokineChinese herbal compound Naofucong
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