Effect of Yiqi Tongyang Decoction (益气通阳方) on blood T cell subsets in patients with chronic immune thrombocytopenia

Xiu-peng Yang; Rou Ma; Xiao-hong Yang; Hui-lan Zhu; Yong-gang Xu

doi:10.1007/s11655-016-2278-7

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Effect of Yiqi Tongyang Decoction (益气通阳方) on blood T cell subsets in patients with chronic immune thrombocytopenia

OriginalPaper | Updated：2021-08-27

- Effect of Yiqi Tongyang Decoction (益气通阳方) on blood T cell subsets in patients with chronic immune thrombocytopenia
- Effect of Yiqi Tongyang Decoction (益气通阳方) on blood T cell subsets in patients with chronic immune thrombocytopenia
- 中国结合医学杂志（英文版） 2017年23卷第9期页码：709-713
- Affiliations：
  
  Department of Hematology, Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81072928);Conflict of Interest;There is no conflict of interest in study.;Author Contribution;The work presented here was carried out in collaboration between all authors. Ma R and Xu YG defined the research theme. Yang XH, Zhu HL, Xu YG and Yang XP, designed methods and experiments, carried out the laboratory experiments. Yang XP and Xu YG analyzed the data, interpreted the results and wrote the paper. Ma R and Xu YG co-designed experiments, discussed analyses, interpretation, and presentation. All authors have approved the final manuscript.
- DOI：10.1007/s11655-016-2278-7
  中图分类号：
- 纸质出版日期：2017，
  
  网络出版日期：2016-12-9，
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Yang, Xp., Ma, R., Yang, Xh. et al. Effect of Yiqi Tongyang Decoction (益气通阳方) on blood T cell subsets in patients with chronic immune thrombocytopenia., Chin. J. Integr. Med. 23, 709–713 (2017). https://doi.org/10.1007/s11655-016-2278-7

Xiu-peng Yang, Rou Ma, Xiao-hong Yang, et al. Effect of Yiqi Tongyang Decoction (益气通阳方) on blood T cell subsets in patients with chronic immune thrombocytopenia[J]. Chinese Journal of Integrative Medicine, 2017,23(9):709-713.
Yang, Xp., Ma, R., Yang, Xh. et al. Effect of Yiqi Tongyang Decoction (益气通阳方) on blood T cell subsets in patients with chronic immune thrombocytopenia., Chin. J. Integr. Med. 23, 709–713 (2017). https://doi.org/10.1007/s11655-016-2278-7 DOI：

Xiu-peng Yang, Rou Ma, Xiao-hong Yang, et al. Effect of Yiqi Tongyang Decoction (益气通阳方) on blood T cell subsets in patients with chronic immune thrombocytopenia[J]. Chinese Journal of Integrative Medicine, 2017,23(9):709-713. DOI： 10.1007/s11655-016-2278-7.

摘要

To measure the proportions of blood T cell subsets

Th1

Th2

Th17

Th22

and Treg cells

and other parameters in patients with chronic immune thrombocytopenia (CITP) before and after treatment with Yiqi Tongyang Decoction (益气通阳方

YTD) to explore T cell status of patients with CITP

and to defifine the mechanism of action of YTD. The changes in peripheral blood T lymphocyte subsets

and those of Th1

Th2

Th17

Th22

and Treg cells in 30 patients with CITP (22 females and 8 males) were analyzed using multiparametric flflow cytometry before and after treatment with YTD for 6 months

and 26 healthy volunteers (14 males and 12 females) acted as a control. T-box expressed in T-cells (T-bet) and GATA binding protein 3 (GATA-3) mRNA levels in patients and controls were analyzed using real-time reverse transcription-polymerase chain reaction. The proportions of Th1

Th17

Th22

Th1/Th2

and Th17/Treg cells increased in the peripheral blood of patients with CITP compared to those in controls before YTD therapy (P<0.05). Th1 cell numbers and the Th1/Th2 ratio fell in the treated patients with CITP to approximate the values of the control group (P>0.05). Th17 cell numbers and the Th17/Treg ratio also decreased in the treatment group (P<0.05)

but not to the levels of the controls. The number of Treg cells in the peripheral blood of patients with CITP before treatment was lower than that in the control group (P<0.05)

but increased after YTD treatment P<0.05)

but not to the level of controls. T-bet and GATA-3 mRNA levels in peripheral blood were initially higher in patients before treatment than controls (P<0.05)

but decreased after YTD therapy (P<0.05). Imbalances in T lymphocyte levels

particularly those of Th1/Th2 and Th17/Treg cells

play important roles in the pathogenesis of CITP. YTD effificiently regulated the dynamics of Th1/Th2 and Th17/Treg equilibria.

Abstract

To measure the proportions of blood T cell subsets

Th1

Th2

Th17

Th22

and Treg cells

and other parameters in patients with chronic immune thrombocytopenia (CITP) before and after treatment with Yiqi Tongyang Decoction (益气通阳方

YTD) to explore T cell status of patients with CITP

and to defifine the mechanism of action of YTD. The changes in peripheral blood T lymphocyte subsets

and those of Th1

Th2

Th17

Th22

and Treg cells in 30 patients with CITP (22 females and 8 males) were analyzed using multiparametric flflow cytometry before and after treatment with YTD for 6 months

Th17

Th22

Th1/Th2

but not to the levels of the controls. The number of Treg cells in the peripheral blood of patients with CITP before treatment was lower than that in the control group (P<0.05)

but increased after YTD treatment P<0.05)

but not to the level of controls. T-bet and GATA-3 mRNA levels in peripheral blood were initially higher in patients before treatment than controls (P<0.05)

but decreased after YTD therapy (P<0.05). Imbalances in T lymphocyte levels

particularly those of Th1/Th2 and Th17/Treg cells

play important roles in the pathogenesis of CITP. YTD effificiently regulated the dynamics of Th1/Th2 and Th17/Treg equilibria.

关键词

immune thrombocytopeniaChinese MedicineYiqi Tongyang DecoctionT-box expressed in T-cellsGATA binding protein 3Th cellsTreg cells

Keywords

immune thrombocytopeniaChinese MedicineYiqi Tongyang DecoctionT-box expressed in T-cellsGATA binding protein 3Th cellsTreg cells

references

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Hu Y, Li H, Zhang L, Shan B, Xu X, Li H, et al. Elevated profiles of Th22 cells and correlations with Th17 cells in patients with immune thrombocytopenia. Hum Immunol 2012;73:629–635.

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