Protective Effects of Danlou Tablet (丹蒌片) against Murine Myocardial Ischemia and Reperfusion Injury In Vivo

Jian-yong Qi; Lei Wang; Dong-sheng Gu; Li-heng Guo; Wei Zhu; Min-zhou Zhang

doi:10.1007/s11655-016-2448-7

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Protective Effects of Danlou Tablet (丹蒌片) against Murine Myocardial Ischemia and Reperfusion Injury In Vivo

OriginalPaper | Updated：2021-08-27

- Protective Effects of Danlou Tablet (丹蒌片) against Murine Myocardial Ischemia and Reperfusion Injury In Vivo
- Protective Effects of Danlou Tablet (丹蒌片) against Murine Myocardial Ischemia and Reperfusion Injury In Vivo
- 中国结合医学杂志（英文版） 2018年24卷第8期页码：613-620
- Affiliations：
  
  1. Intensive Care Laboratory, Guangdong Province Hospital of Chinese Medicine, The 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou,China
  2. Department of Rheumatology, The 3rd Affiliated Hospital of Southern Medical University,Guangzhou,China
  3. Network Pharmacology, Guangdong Province Hospital of Chinese Medicine, The 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou,China
- Author bio：
- Funds：
  
  Supported by Guangdong National Scientific Funding (No. 2014A030313402), Guangdong Medical Research Foundation (No. A2014271), National Natural Science Foundation of China (No. 81473471 and No. 81573708)
- DOI：10.1007/s11655-016-2448-7
  中图分类号：
- 纸质出版日期：2018，
  
  网络出版日期：2016-3-21，
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Qi, Jy., Wang, L., Gu, Ds. et al. Protective Effects of Danlou Tablet (丹蒌片) against Murine Myocardial Ischemia and Reperfusion Injury In Vivo., Chin. J. Integr. Med. 24, 613–620 (2018). https://doi.org/10.1007/s11655-016-2448-7

Jian-yong Qi, Lei Wang, Dong-sheng Gu, et al. Protective Effects of Danlou Tablet (丹蒌片) against Murine Myocardial Ischemia and Reperfusion Injury In Vivo[J]. Chinese Journal of Integrative Medicine, 2018,24(8):613-620.
Qi, Jy., Wang, L., Gu, Ds. et al. Protective Effects of Danlou Tablet (丹蒌片) against Murine Myocardial Ischemia and Reperfusion Injury In Vivo., Chin. J. Integr. Med. 24, 613–620 (2018). https://doi.org/10.1007/s11655-016-2448-7 DOI：

Jian-yong Qi, Lei Wang, Dong-sheng Gu, et al. Protective Effects of Danlou Tablet (丹蒌片) against Murine Myocardial Ischemia and Reperfusion Injury In Vivo[J]. Chinese Journal of Integrative Medicine, 2018,24(8):613-620. DOI： 10.1007/s11655-016-2448-7.

摘要

To observe the in vivo effect of Danlou Tablet (丹蒌片

DLT) on myocardial ischemia and reperfusion (I/R) injury. DLT effects were evaluated in mouse heart preparation using 30-min coronary occlusion followed by 24-h reperfusion and compared among sham group (n=6)

I/R group (n=8)

IPC group (ischemia preconditioning

n=6) and DLT group (I/R with DLT pretreatment for 3 days

750 mg•kg-1•day-1

n=8). The effects of DLT were characterized in infarction size (IS) compared with risk region (RR) and left ventricle using the Evans blue/triphenyltetrazolium chloride double dye staining method in vivo. Furthermore

the dose-dependent effect of DLT on I/R injury was evaluated by double staining method. Five different concentrations of DLT (0.625

1.25

2.5

5 and 10 g•kg-1•day-1) were chosen in this study

and dose-response curve of DLT was obtained on these data. The ratio of IS to left ventricle was significantly smaller in the DLT and IPC groups than the I/R group (P<0.05 or P<0.01)

the ratio of IS to RR was also reduced in the DLT and IPC groups (P<0.01)

while there were no differences in RR among the four groups (P>0.05). Experiments showed incidence of arrhythmias was reduced in the DLT group (P<0.01). Furthermore

DLT produced a dose-dependent inhibitory effect with a half maximal inhibitory concentration of 1.225 g•kg-1•day-1. Our research concluded that DLT was effective in reducing I/R injury in mice

and provided experimental supports for the clinical use of DLT.

Abstract

To observe the in vivo effect of Danlou Tablet (丹蒌片

I/R group (n=8)

IPC group (ischemia preconditioning

n=6) and DLT group (I/R with DLT pretreatment for 3 days

750 mg•kg-1•day-1

the dose-dependent effect of DLT on I/R injury was evaluated by double staining method. Five different concentrations of DLT (0.625

1.25

2.5

5 and 10 g•kg-1•day-1) were chosen in this study

and dose-response curve of DLT was obtained on these data. The ratio of IS to left ventricle was significantly smaller in the DLT and IPC groups than the I/R group (P<0.05 or P<0.01)

the ratio of IS to RR was also reduced in the DLT and IPC groups (P<0.01)

while there were no differences in RR among the four groups (P>0.05). Experiments showed incidence of arrhythmias was reduced in the DLT group (P<0.01). Furthermore

DLT produced a dose-dependent inhibitory effect with a half maximal inhibitory concentration of 1.225 g•kg-1•day-1. Our research concluded that DLT was effective in reducing I/R injury in mice

and provided experimental supports for the clinical use of DLT.

关键词

ischemia and reperfusionChinese MedicineDanlou Tablet

Keywords

ischemia and reperfusionChinese MedicineDanlou Tablet

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