Effect of Total Flavone of Haw Leaves on Nuclear Factor Erythroid-2 Related Factor and Other Related Factors in Nonalcoholic Steatohepatitis Rats

De-jun Wang; Yue-qin Cai; Shui-zhen Pan; Li-zong Zhang; Yun-xiang Chen; Fang-ming Chen; Ming Jin; Mao-xiang Yan; Xiao-dong Li; Zhi-yun Chen

doi:10.1007/s11655-016-2450-0

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Effect of Total Flavone of Haw Leaves on Nuclear Factor Erythroid-2 Related Factor and Other Related Factors in Nonalcoholic Steatohepatitis Rats

OriginalPaper | Updated：2021-08-27

- Effect of Total Flavone of Haw Leaves on Nuclear Factor Erythroid-2 Related Factor and Other Related Factors in Nonalcoholic Steatohepatitis Rats
- Effect of Total Flavone of Haw Leaves on Nuclear Factor Erythroid-2 Related Factor and Other Related Factors in Nonalcoholic Steatohepatitis Rats
- 中国结合医学杂志（英文版） 2018年24卷第4期页码：265-271
- Affiliations：
  
  1. Laboratory Animal Research Center, Zhejiang Chinese Medical University,Hangzhou,China
  2. Drug Safety Evaluation Center, Zhejing Academy of Medical Sciences,Hangzhou,China
  3. The First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-016-2450-0
  中图分类号：
- 纸质出版日期：2018，
  
  网络出版日期：2016-2-26，
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Wang, Dj., Cai, Yq., Pan, Sz. et al. Effect of Total Flavone of Haw Leaves on Nuclear Factor Erythroid-2 Related Factor and Other Related Factors in Nonalcoholic Steatohepatitis Rats., Chin. J. Integr. Med. 24, 265–271 (2018). https://doi.org/10.1007/s11655-016-2450-0

De-jun Wang, Yue-qin Cai, Shui-zhen Pan, et al. Effect of Total Flavone of Haw Leaves on Nuclear Factor Erythroid-2 Related Factor and Other Related Factors in Nonalcoholic Steatohepatitis Rats[J]. Chinese Journal of Integrative Medicine, 2018,24(4):265-271.
Wang, Dj., Cai, Yq., Pan, Sz. et al. Effect of Total Flavone of Haw Leaves on Nuclear Factor Erythroid-2 Related Factor and Other Related Factors in Nonalcoholic Steatohepatitis Rats., Chin. J. Integr. Med. 24, 265–271 (2018). https://doi.org/10.1007/s11655-016-2450-0 DOI：

De-jun Wang, Yue-qin Cai, Shui-zhen Pan, et al. Effect of Total Flavone of Haw Leaves on Nuclear Factor Erythroid-2 Related Factor and Other Related Factors in Nonalcoholic Steatohepatitis Rats[J]. Chinese Journal of Integrative Medicine, 2018,24(4):265-271. DOI： 10.1007/s11655-016-2450-0.

摘要

To investigate the effect of total flavone of haw leaves (TFHL) on the expression of nuclear factor erythroid-2 related factor (Nrf2) and other related factors in nonalcoholic steatohepatitis (NASH) rats induced by high-fat diet and then to further discuss the mechanism of TFHL’s prevention against NASH. High-fat diet was fed to 40 rats to establish the NASH model. Then model rats were intragastrically administrated with 40

160 mg/(kg•day) TFHL

respectively. The pathological changes of liver tissues in NASH rats were detected by oil red O and hematoxylin-eosin (HE) stainings. The expression of Nrf2 in rat liver was examined through immunohistochemistry. The level of 8-iso-prostaglandin F2α in serum was detected through enzyme linked immunosorbent assay (ELISA). The mRNA and protein levels of Nrf2 and other related factors in liver tissue were measured by real-time reverse transcriptionpolymerase chain reaction and western blot. Lipid deposition

hepatic steatosis

focal necrosis in lobular inflammation and ballooning degeneration were emerged in livers of NASH rats. The 8-iso-prostaglandin F2α in the serum of NASH rats increased significantly compared with the control group (P<0.05). The mRNA of Nrf2

hemeoxyenase1 (HO-1) and the mRNA and protein levels of quinine oxidoreductase (NQO1) in NASH rats liver tissue showed a striking increase

while the mRNA levels of Keap1

r-glutamylcysteine synthethase (rGCS) and glutathione S-transferase (GST) were significantly decreased compared with the control group (P<0.05). After TFHL treatment

8-iso-prostaglandin F2α level in serum significantly decreased

and Nrf2 mRNA and protein levels in hepatocytes nucleus enhanced compared with the model group (P<0.05 or 0.01). Meanwhile the Keap1 mRNA

the mRNA and protein levels of HO-1

NQO1 antibody

rGCS antibody

GST increased after TFHL treatment (P<0.05 or 0.01). Nrf2 and other related factors were involved in development of NASH

and they also served as an important part in its occurrence. By regulating expression of Nrf2 and other related factors

TFHL may play a role in antioxidative stress and prevention of NASH.

Abstract

160 mg/(kg•day) TFHL

hepatic steatosis

hemeoxyenase1 (HO-1) and the mRNA and protein levels of quinine oxidoreductase (NQO1) in NASH rats liver tissue showed a striking increase

while the mRNA levels of Keap1

r-glutamylcysteine synthethase (rGCS) and glutathione S-transferase (GST) were significantly decreased compared with the control group (P<0.05). After TFHL treatment

8-iso-prostaglandin F2α level in serum significantly decreased

and Nrf2 mRNA and protein levels in hepatocytes nucleus enhanced compared with the model group (P<0.05 or 0.01). Meanwhile the Keap1 mRNA

the mRNA and protein levels of HO-1

NQO1 antibody

rGCS antibody

GST increased after TFHL treatment (P<0.05 or 0.01). Nrf2 and other related factors were involved in development of NASH

and they also served as an important part in its occurrence. By regulating expression of Nrf2 and other related factors

TFHL may play a role in antioxidative stress and prevention of NASH.

关键词

nonalcoholic steatohepatitistotal flavone of Haw leavesnuclear factor erythroid-2 related factor

Keywords

nonalcoholic steatohepatitistotal flavone of Haw leavesnuclear factor erythroid-2 related factor

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