Effect of EphB4/EphrinB2 reverse signal on angiogenesis induced by Xuefu Zhuyu Capsule (血府逐瘀胶囊) containing serum in human microvascular endothelial cell 1

Jing-si Zhang; Yi-zheng Wang; Ya-qiong Hu; Fan Lin; Dong Gao; Jun Song; Ted J. Kaptchuk; Ke-ji Chen

doi:10.1007/s11655-016-2470-9

Your Location：

Home >

Browse articles >

Effect of EphB4/EphrinB2 reverse signal on angiogenesis induced by Xuefu Zhuyu Capsule (血府逐瘀胶囊) containing serum in human microvascular endothelial cell 1

OriginalPaper | Updated：2021-08-27

- Effect of EphB4/EphrinB2 reverse signal on angiogenesis induced by Xuefu Zhuyu Capsule (血府逐瘀胶囊) containing serum in human microvascular endothelial cell 1
- Effect of EphB4/EphrinB2 reverse signal on angiogenesis induced by Xuefu Zhuyu Capsule (血府逐瘀胶囊) containing serum in human microvascular endothelial cell 1
- 中国结合医学杂志（英文版） 2016年22卷第8期页码：605-610
- Affiliations：
  
  1. Zhongshan Hospital, Fudan University,Shanghai,China
  2. College of Integrative Medicine, Fujian University of Traditional Chinese Medicine,Fuzhou,China
  3. Experimental Research Center, China Academy of Chinese Medical Sciences,Beijing,China
  4. Beth Israel Deaconess Medical Center, Harvard Medical School,Boston,USA
  5. Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81072933 and No. 81173431);and National Center for Complementary and Alternative Medicine/ National Institutes of Health of USA (No. 2K24 AT004095)
- DOI：10.1007/s11655-016-2470-9
  中图分类号：
- 纸质出版日期：2016，
  
  网络出版日期：2016-6-14，
Scan for full text
Zhang, Js., Wang, Yz., Hu, Yq. et al. Effect of EphB4/EphrinB2 reverse signal on angiogenesis induced by Xuefu Zhuyu Capsule (血府逐瘀胶囊) containing serum in human microvascular endothelial cell 1., Chin. J. Integr. Med. 22, 605–610 (2016). https://doi.org/10.1007/s11655-016-2470-9

Jing-si Zhang, Yi-zheng Wang, Ya-qiong Hu, et al. Effect of EphB4/EphrinB2 reverse signal on angiogenesis induced by Xuefu Zhuyu Capsule (血府逐瘀胶囊) containing serum in human microvascular endothelial cell 1[J]. Chinese Journal of Integrative Medicine, 2016,22(8):605-610.
Zhang, Js., Wang, Yz., Hu, Yq. et al. Effect of EphB4/EphrinB2 reverse signal on angiogenesis induced by Xuefu Zhuyu Capsule (血府逐瘀胶囊) containing serum in human microvascular endothelial cell 1., Chin. J. Integr. Med. 22, 605–610 (2016). https://doi.org/10.1007/s11655-016-2470-9 DOI：

Jing-si Zhang, Yi-zheng Wang, Ya-qiong Hu, et al. Effect of EphB4/EphrinB2 reverse signal on angiogenesis induced by Xuefu Zhuyu Capsule (血府逐瘀胶囊) containing serum in human microvascular endothelial cell 1[J]. Chinese Journal of Integrative Medicine, 2016,22(8):605-610. DOI： 10.1007/s11655-016-2470-9.

摘要

To evaluate the effect of Xuefu Zhuyu Capsule (血府逐瘀胶囊)-containing serum (XFZY-CS) on EphB4/ephrinB2 and its reverse signal in human microvascular endothelial cell-1 (HMEC-1). XFZY-CS and the blank control serum were collected. HMEC-1 cells were randomly assigned to 6 groups including the concentration 1.25%

2.5%

and 5% XFZY-CS groups and their blank serum control ones. The angiogenesis effect of XFZY-CS was tested with an in vitro tube formation assay and the best condition of pro-angiogenesis was determined. The effect of XFZY-CS on EphB4/ephrinB2 and the reverse signal were determined by Western blot and real-time quantitative polymerase chain reaction

respectively; we also confifirmed the results through activating and inhibiting the reverse signal by EphB4/fc and pyrophosphatase/ phosphodiesterase2 (PP2). XFZY-CS promoted angiogenesis at the concentration of 2.5% corresponding serum after being cultured for 48 h

while inhibited angiogenesis at the concentration of 5% after culturing for 48 and 72 h. Under the 2.5% serum concentration

XFZY up-regulated the expression of EphB4-mRNA at 12 h (P<0.05)

and down-regulates its expression at 24 h (P<0.01). Protein expression of EphB4 was apparently up-regulated at 12 h and down-regulated at 24 h. The phosphorylation of ephrinB2 increased at 9 h (P<0.05). In addition

2.5% XFZY-CS played a similar role as the reverse signaling activator EphB4/Fc ranging from 0.5 to 5 μg/mL (P>0.05). XFZY-CS also reduced the inhibitive effect of PP2 in limited periods. EphB4/ephrinB2 was the upstream signal in the process of angiogenesis and its reverse signaling was responsible for XFZY’s effect on promoting angiogenesis.

Abstract

2.5%

while inhibited angiogenesis at the concentration of 5% after culturing for 48 and 72 h. Under the 2.5% serum concentration

XFZY up-regulated the expression of EphB4-mRNA at 12 h (P<0.05)

关键词

Xuefu Zhuyu CapsuleangiogenesisephrinB2/EphB4 reverse signal

Keywords

Xuefu Zhuyu CapsuleangiogenesisephrinB2/EphB4 reverse signal

references

Gao D, Wu LY, Jiao YH, Chen WY. The effect of Xuefu Zhuyu Decoction on in vitro endothelial progenitor cell tube formation. Chin J Integr Med 2010;16:50–53.

Song J, Chen WY, Wu LY, Zheng LP. A microarray analysis of angiogenesis modulation effect of Xuefu Zhuyu Decoction on endothelial cells. Chin J Integr Med 2012;18:502–506.

Eter N, Spitznas M. DMSO mimics inhibitory effect of thalidomide on choriocapillary endothelial cell proliferation in culture. Br J Ophthalmol 2002;86:1303–1305.

Brigit M, Bettina R, Christin N. Eph receptors and ephrin ligands: important player in angiogenesis and tumor angiogenesis. Biochim Biophys Acta 2005;1723:1–7.

Murai KK, Pasquale EB. Eph’ective signaling: forward, reverse and crosstalk. J Cell Sci 2003;116:2823–2832.

Taylor PP, Biins KL, Sicheri F. Phosphorylation of tyrosine residues in the kinase domain and juxtamembrane region regulates the biological and catalytic activities of Eph receptors. Mol Cell Biol 2000;20:4791–4805.

Wang HU, Chen ZF, Anderson DJ. Molecular distinction and angiogenesis interaction between embryonic arteries and veins revealed by ephrinB2 and its receptor EphB4. Cell 1998;93:741–753.

Adams RH, Wiikinson GA, Weiss C. Roles of ephrinB ligands and EphB receptors in cardiovascular development: demarcation of arterial/venous domain, vscular morphogenesis, and sprouting angiogenesis. Genes Dev 1999;93:661–664.

Gale NW, Baluk P, Pan L. EphrinB2 selectively marks arterial vessels and neovascularization sites in the adult, with expression in both endothelial and smooth muscles cells. Dev Biol 2001;230:151–160.

Shin D, Hayashi S, Gerety S. Expression of ephrinB2 identifies a stable genetic difference between arterial and venous vascular smooth muscle as well as endothelial cells, and marks subsets of microvessels at sites of adult neovascularization. Dev Biol 2001;230:139–150.

Hamada K, Oile Y, Ito Y. Distinct roles of ephrinB2 forward and EphB4 reverse signaling in endothelial cells. Arterioscler thromb Vasc Biol 2003;23:190–197.

Kida Y, Ieronimakis N, Schrimpf C. EphrinB2 reverse signaling protecrs against capillary rarefaction and fibrosis after kidney injury. J Am Soc Nephol 2013;24:559–572.

Taylor AC, Mendel TA, Mason KE. Attenuation of ephrinB2 reverse signaling decreases vascularized area and preretinal vascular tuft formation in the murine model of oxygen-induced retinopathy. Invest Ophthalmol Vis Sci 2012;53:5462–5470.

Gao D, Chen WY, Wu LY. Effect of Xuefu Zhuyu Decoction in inducing angiogenesis gene regulation of endothelial cell line ECV304. Chin J Integr Tradit West Med (Chin) 2010;30:153–156.

Bochenek ML, Dicklson S, Astin JW. EphrinB2 regulates endothelial cell morphology and motility independently of Eph-receptor binding. J Cell Sci 2010;123:1235–1246.

Gao D, Lin W, Zheng LP. Effect of Xuefu Zhuyu Decoction on endothelial progenitor cells migrating from rat marrow. Chin J Integr Med Cardio-/Cerebrovasc Dis (Chin) 2007;5:829–831.

Gao D, Wu LY, Jiao YH, Chen WY. Effect of vegf-vegfr pathway on EPCs function induced by Xuefu Zhuyu Decoction. Chin J Experi Tradit Med Formu (Chin) 2010;16:104–107.

FULL TEXT LINK

More>

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Inhibitory effect of cryptotanshinone on angiogenesis and Wnt/β-catenin signaling pathway in human umbilical vein endothelial cells

Electroacupuncture at Zusanli (ST36) accelerates intracerebral hemorrhage-induced angiogenesis in rats

Bone marrow angiogenesis in patients presenting with differential Chinese medicine syndrome: Correlation with the clinico-pathological features of aplastic anemia

Angiogenesis opens a way for Chinese medicine to treat stroke

Anticancer effects of 5-fluorouracil combined with warming and relieving cold phlegm formula on human breast cancer