Seropharmacological study on osteogenic effects of post-absorption ingredients of an osteoprotective herbal formula

Wing-sum Siu; Chun-hay Ko; Hing-lok Wong; Si Gao; Wai-ting Shum; Clara Bik-san Lau; Lung-kim Hung; Ping-chung Leung

doi:10.1007/s11655-016-2474-5

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Seropharmacological study on osteogenic effects of post-absorption ingredients of an osteoprotective herbal formula

OriginalPaper | Updated：2021-08-27

- Seropharmacological study on osteogenic effects of post-absorption ingredients of an osteoprotective herbal formula
- Seropharmacological study on osteogenic effects of post-absorption ingredients of an osteoprotective herbal formula
- 中国结合医学杂志（英文版） 2017年23卷第1期页码：25-32
- Affiliations：
  
  1. Institute of Chinese Medicine, Chinese University of Hong Kong,Hong Kong,China
  2. State Key Laboratory of Phytochemistry and Plant Resources in West China, Chinese University of Hong Kong,Hong Kong,China
  3. Department of Orthopaedics and Traumatology, Chinese University of Hong Kong,Hong Kong,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-016-2474-5
  中图分类号：
- 纸质出版日期：2017，
  
  网络出版日期：2016-6-14，
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Siu, Ws., Ko, Ch., Wong, Hl. et al. Seropharmacological study on osteogenic effects of post-absorption ingredients of an osteoprotective herbal formula., Chin. J. Integr. Med. 23, 25–32 (2017). https://doi.org/10.1007/s11655-016-2474-5

Wing-sum Siu, Chun-hay Ko, Hing-lok Wong, et al. Seropharmacological study on osteogenic effects of post-absorption ingredients of an osteoprotective herbal formula[J]. Chinese Journal of Integrative Medicine, 2017,23(1):25-32.
Siu, Ws., Ko, Ch., Wong, Hl. et al. Seropharmacological study on osteogenic effects of post-absorption ingredients of an osteoprotective herbal formula., Chin. J. Integr. Med. 23, 25–32 (2017). https://doi.org/10.1007/s11655-016-2474-5 DOI：

Wing-sum Siu, Chun-hay Ko, Hing-lok Wong, et al. Seropharmacological study on osteogenic effects of post-absorption ingredients of an osteoprotective herbal formula[J]. Chinese Journal of Integrative Medicine, 2017,23(1):25-32. DOI： 10.1007/s11655-016-2474-5.

摘要

To further investigate the {ptin vitro} effects of an osteoprotective herbal formula "ELP" (Herba Epimedii

Fructus Ligustri Lucidi and Fructus Psoraleae) using seropharmacological approach. Rats were fed with ELP or its individual component herbs for 2 days. The serum containing the postabsorbed ingredients of the herbal items were collected for cell culture using UMR106 cell

RAW264.7 cell and mesenchymal stem cell (MSC) isolated from the bone marrow of the rats. The effects of the herbal-containing serum on cell toxicity were detected by 3-[4

5-dimethylthiazol-2-yl]-2

5-diphenyltetrazolium bromide assay; bromodeoxyuridine assay was conducted to measure the cell proliferation of UMR106 cell and MSC; cell activity was measured using colorimetric method

and mRNA expression of runt-related transcription factor 2 (Runx2)

alkaline phosphatase (ALP) and osteopontin (OPN) of UMR106 and MSC as well as matrix metalloproteinase 9 (MMP-9)

tartrate-resistant acid phosphatase (TRAP) and cathepsin K of RAW264.7 were analyzed using real-time reverse-transcription polymerase chain reaction. ELP and its component serum exhibited no cytotoxic effects on the cells. The ELP-containing serum increased the proliferation of UMR106 cell and MSC by 25.7% and 14.4 %

respectively and the alkaline phosphatase activity of MSC was increased by 42.6%. On the contrary

it inhibited the RAW264.7 cell differentiation by 29.2 %. ELP serum upregulated the Runx2 expression of UMR and MSC by 1.18 fold and 1.27 fold

respectively. It also upregulated ALP and OPN expression in MSC by 1.69- and 2.12-fold

respectively. On the other hand

ELP serum down-regulated MMP-9 and cathepsin K expression of RAW264.7 cell by 0.46- and 0.36-fold

respectively. The serum of the animals fed with ELP contains active ingredients which are effective in promoting osteogenesis and inhibiting osteoclastogenesis.

Abstract

To further investigate the {ptin vitro} effects of an osteoprotective herbal formula "ELP" (Herba Epimedii

RAW264.7 cell and mesenchymal stem cell (MSC) isolated from the bone marrow of the rats. The effects of the herbal-containing serum on cell toxicity were detected by 3-[4

5-dimethylthiazol-2-yl]-2

5-diphenyltetrazolium bromide assay; bromodeoxyuridine assay was conducted to measure the cell proliferation of UMR106 cell and MSC; cell activity was measured using colorimetric method

and mRNA expression of runt-related transcription factor 2 (Runx2)

alkaline phosphatase (ALP) and osteopontin (OPN) of UMR106 and MSC as well as matrix metalloproteinase 9 (MMP-9)

respectively and the alkaline phosphatase activity of MSC was increased by 42.6%. On the contrary

it inhibited the RAW264.7 cell differentiation by 29.2 %. ELP serum upregulated the Runx2 expression of UMR and MSC by 1.18 fold and 1.27 fold

respectively. It also upregulated ALP and OPN expression in MSC by 1.69- and 2.12-fold

respectively. On the other hand

ELP serum down-regulated MMP-9 and cathepsin K expression of RAW264.7 cell by 0.46- and 0.36-fold

respectively. The serum of the animals fed with ELP contains active ingredients which are effective in promoting osteogenesis and inhibiting osteoclastogenesis.

关键词

OsteoporosisseropharmacologyosteogenesisHerbal Formula

Keywords

OsteoporosisseropharmacologyosteogenesisHerbal Formula

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