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Effects of Danhong Injection (丹红注射液) and its main components on anticoagulation and fibrinolysis in cultured vein endothelial cells
Effects of Danhong Injection (丹红注射液) and its main components on anticoagulation and fibrinolysis in cultured vein endothelial cells
- 中国结合医学杂志（英文版） 2016年22卷第4期 页码：276-283
- Affiliations：
1. Research Institution of Cardio-Cerebral-Vascular Disease, Zhejiang University of Traditional Chinese Medicine,Hangzhou,China
2. Pharmaceutical College, Zhejiang University of Traditional Chinese Medicine,Hangzhou,China
3. School of Food and Pharmaceutical Engineering, Hubei University of Technology,Wuhan,China
- Author bio：
- Funds：Supported by the National Natural Science Foundation of China (No. 81173647, 81473587, 81274176), Zhejiang Provincial Program for the Cultivation of High-Level Innovative Health Talents, and Key Discipline of Zhejiang Traditional Chinese Medicine (Integrated Traditional and Western Medicine, No. 2012-XK-A06)
DOI：10.1007/s11655-016-2498-x
中图分类号：纸质出版日期：2016，
网络出版日期：2016-4-9，
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Zhang, Yy., Zhou, Hf., Yang, Jh. et al. Effects of Danhong Injection (丹红注射液) and its main components on anticoagulation and fibrinolysis in cultured vein endothelial cells., Chin. J. Integr. Med. 22, 276–283 (2016). https://doi.org/10.1007/s11655-016-2498-x
Yu-yan Zhang, Hui-fen Zhou, Jie-hong Yang, et al. Effects of Danhong Injection (丹红注射液) and its main components on anticoagulation and fibrinolysis in cultured vein endothelial cells[J]. Chinese Journal of Integrative Medicine, 2016,22(4):276-283.
Zhang, Yy., Zhou, Hf., Yang, Jh. et al. Effects of Danhong Injection (丹红注射液) and its main components on anticoagulation and fibrinolysis in cultured vein endothelial cells., Chin. J. Integr. Med. 22, 276–283 (2016). https://doi.org/10.1007/s11655-016-2498-x DOI：
Yu-yan Zhang, Hui-fen Zhou, Jie-hong Yang, et al. Effects of Danhong Injection (丹红注射液) and its main components on anticoagulation and fibrinolysis in cultured vein endothelial cells[J]. Chinese Journal of Integrative Medicine, 2016,22(4):276-283. DOI： 10.1007/s11655-016-2498-x.
摘要
To observe the effects of Danhong Injection (丹红注射液) and its main components
including daiclzein and hydroxysafflor yellow A (HSYA)
on the anticoagulation
fibrinolysis
anti-apoptosis in hypoxia model of vein endothelial cells (VECs). VECs were prepared and were put in a hypoxia environment
which consisted of mixed gas of 95% N and 5% CO mixed gas
when reached confluent culture. Five groups used different treatments
including normal control group
hypoxia group
daiclzein group
HSYA group and Danhong Injection group. The VECs were identified by fluorescence double labeling methods. The morphology was observed by a phase contrast microscopy. The effects of Danhong Injection
daiclzein and HSYA on 6 keto prostaglandin F1α (6-keto-PGF1α) level was measured by the method of radioimmunoassay (RIA). Superoxide dismutase (SOD) activity was tested by water soluble tetrazolium salt. The content of malondialdehyde (MDA) was measured by thiobarbituric acid. The activities of tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI) were measured by the method of chromogenic substrate. The contents of endothelin (ET) and nitric oxide (NO) were detected by non-equilibrium RIA and enzymelinked immunosorbent assay. Cells apoptosis rate was determined by flow cytometry. Compared with the normal control group
the floating cells number
PAI activity
ET and MDA contents
and cells apoptosis rate in the culture solution of hypoxia group were all significantly increased
whereas the 6-keto-PGF1α and NO contents
and t-PA and SOD activities were decreased significantly (P<0.01). Compared with the hypoxia group
Danhong Injection markedly increased the 6-keto-PGF1α content and SOD activity
regulated PAI and t-PA activities
ET and NO contents
and decreased MDA content and cells apoptosis rate (P<0.05 or P<0.01). Danhong Injection and its main components played an important role in protecting primary VECs from hypoxic damage by regulating the secretion and vasomotor function of VECs. The function of Danhong Injection was most remarkable.
Abstract
To observe the effects of Danhong Injection (丹红注射液) and its main components
including daiclzein and hydroxysafflor yellow A (HSYA)
on the anticoagulation
fibrinolysis
anti-apoptosis in hypoxia model of vein endothelial cells (VECs). VECs were prepared and were put in a hypoxia environment
which consisted of mixed gas of 95% N and 5% CO mixed gas
when reached confluent culture. Five groups used different treatments
including normal control group
hypoxia group
daiclzein group
HSYA group and Danhong Injection group. The VECs were identified by fluorescence double labeling methods. The morphology was observed by a phase contrast microscopy. The effects of Danhong Injection
daiclzein and HSYA on 6 keto prostaglandin F1α (6-keto-PGF1α) level was measured by the method of radioimmunoassay (RIA). Superoxide dismutase (SOD) activity was tested by water soluble tetrazolium salt. The content of malondialdehyde (MDA) was measured by thiobarbituric acid. The activities of tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI) were measured by the method of chromogenic substrate. The contents of endothelin (ET) and nitric oxide (NO) were detected by non-equilibrium RIA and enzymelinked immunosorbent assay. Cells apoptosis rate was determined by flow cytometry. Compared with the normal control group
the floating cells number
PAI activity
ET and MDA contents
and cells apoptosis rate in the culture solution of hypoxia group were all significantly increased
whereas the 6-keto-PGF1α and NO contents
and t-PA and SOD activities were decreased significantly (P<0.01). Compared with the hypoxia group
Danhong Injection markedly increased the 6-keto-PGF1α content and SOD activity
regulated PAI and t-PA activities
ET and NO contents
and decreased MDA content and cells apoptosis rate (P<0.05 or P<0.01). Danhong Injection and its main components played an important role in protecting primary VECs from hypoxic damage by regulating the secretion and vasomotor function of VECs. The function of Danhong Injection was most remarkable.
关键词
vein endothelial cellsDanhong Injectiondaiclzeinhydroxysafflor yellow AanticoagulationfibrinolysisChinese Medicine
Keywords
vein endothelial cellsDanhong Injectiondaiclzeinhydroxysafflor yellow AanticoagulationfibrinolysisChinese Medicine
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