Effects of Shen-Fu Injection (参附注射液) on apoptosis of regulatory T lymphocytes in spleen during post-resuscitation immune dysfunction in a porcine model of cardiac arrest

Wei Gu; Qian Zhang; Chun-sheng Li

doi:10.1007/s11655-016-2505-2

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Effects of Shen-Fu Injection (参附注射液) on apoptosis of regulatory T lymphocytes in spleen during post-resuscitation immune dysfunction in a porcine model of cardiac arrest

OriginalPaper | Updated：2021-08-27

- Effects of Shen-Fu Injection (参附注射液) on apoptosis of regulatory T lymphocytes in spleen during post-resuscitation immune dysfunction in a porcine model of cardiac arrest
- Effects of Shen-Fu Injection (参附注射液) on apoptosis of regulatory T lymphocytes in spleen during post-resuscitation immune dysfunction in a porcine model of cardiac arrest
- 中国结合医学杂志（英文版） 2016年22卷第9期页码：666-673
- Affiliations：
  
  1. Department of Emergency Medicine, Beijing Chaoyang Hospital, Capital Medical University,Beijing,China
  2. Beijing Key Laboratory of Cardiopulmonary Cerebral Resuscitation,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81372025), Basic and Clinical Research Cooperation Project of Capital Medical University (No. 15JL42), and Beijing Municipal Administration of Hospitals Incubating Program (No. Px2016022)
- DOI：10.1007/s11655-016-2505-2
  中图分类号：
- 纸质出版日期：2016，
  
  网络出版日期：2016-9-11，
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Gu, W., Zhang, Q. & Li, Cs. Effects of Shen-Fu Injection (参附注射液) on apoptosis of regulatory T lymphocytes in spleen during post-resuscitation immune dysfunction in a porcine model of cardiac arrest., Chin. J. Integr. Med. 22, 666–673 (2016). https://doi.org/10.1007/s11655-016-2505-2

Wei Gu, Qian Zhang, Chun-sheng Li. Effects of Shen-Fu Injection (参附注射液) on apoptosis of regulatory T lymphocytes in spleen during post-resuscitation immune dysfunction in a porcine model of cardiac arrest[J]. Chinese Journal of Integrative Medicine, 2016,22(9):666-673.
Gu, W., Zhang, Q. & Li, Cs. Effects of Shen-Fu Injection (参附注射液) on apoptosis of regulatory T lymphocytes in spleen during post-resuscitation immune dysfunction in a porcine model of cardiac arrest., Chin. J. Integr. Med. 22, 666–673 (2016). https://doi.org/10.1007/s11655-016-2505-2 DOI：

Wei Gu, Qian Zhang, Chun-sheng Li. Effects of Shen-Fu Injection (参附注射液) on apoptosis of regulatory T lymphocytes in spleen during post-resuscitation immune dysfunction in a porcine model of cardiac arrest[J]. Chinese Journal of Integrative Medicine, 2016,22(9):666-673. DOI： 10.1007/s11655-016-2505-2.

摘要

To investigate whether Shen-Fu Injection (参附注射液

SFI) reduces post-resuscitation immune dysfunction in a porcine model of cardiac arrest by modulating apoptosis of regulatory T lymphocytes (Treg) in the spleen. After 8-min untreated ventricular fibrillation and 2-min basic life support

24 pigs were divided into 3 groups with a random number table

i.e. SFI group

epinephrine (EP) group

and saline (SA) group (8 in each group)

which received central venous injection of SFI (1.0 mL/kg)

EP (0.02 mg/kg) and SA

respectively. The same procedure without CA initiation was achieved in the sham-operated (sham) group (n=6). After successful return of spontaneous circulation (ROSC)

apoptosis rate of splenic Treg was detected by flow cytometry; and the mRNA expression of forkhead/winged helix transcription factor (Foxp3) of splenic Treg was detected by real time-polymerase chain reaction; and the levels of interleukin-4 (IL-4) and interferon-γ (IFN-γ) in porcine splenic Treg were detected by using enzyme-linked immunosorbent assay (ELISA). Compared with the sham group

the apoptosis rate of Treg was significantly decreased

and the levels of Foxp3 mRNA expression

IFN-γ

IL-4 and IFN-γ/IL-4 were increased in the SA group (P<0.05 or P<0.01). Compared with the EP and SA groups

SFI treatment increased the apoptosis rate of Treg and reduced the levels of Foxp3 mRNA expression

IFN-γ and IFN-γ/IL-4 (P<0.05). SFI has signifificant effects in attenuating post-resuscitation immune dysfunction by modulating apoptosis of Treg in the spleen.

Abstract

To investigate whether Shen-Fu Injection (参附注射液

24 pigs were divided into 3 groups with a random number table

i.e. SFI group

epinephrine (EP) group

and saline (SA) group (8 in each group)

which received central venous injection of SFI (1.0 mL/kg)

EP (0.02 mg/kg) and SA

respectively. The same procedure without CA initiation was achieved in the sham-operated (sham) group (n=6). After successful return of spontaneous circulation (ROSC)

the apoptosis rate of Treg was significantly decreased

and the levels of Foxp3 mRNA expression

IFN-γ

IL-4 and IFN-γ/IL-4 were increased in the SA group (P<0.05 or P<0.01). Compared with the EP and SA groups

SFI treatment increased the apoptosis rate of Treg and reduced the levels of Foxp3 mRNA expression

IFN-γ and IFN-γ/IL-4 (P<0.05). SFI has signifificant effects in attenuating post-resuscitation immune dysfunction by modulating apoptosis of Treg in the spleen.

关键词

Shen-fu Injectioncardiopulmonary resuscitationpost-resuscitation immune dysfunctionregulatory T lymphocytes apoptosisforkhead/winged helix transcription factorChinese Medicine

Keywords

Shen-fu Injectioncardiopulmonary resuscitationpost-resuscitation immune dysfunctionregulatory T lymphocytes apoptosisforkhead/winged helix transcription factorChinese Medicine

references

Nolan JP, Neumar RW, Adrie C, Aibiki M, Berg RA, Bbttiger BW, et al. Post-cardiac arrest syndrome: epidemiology, pathophysiology, treatment, and prognostication. Circulation 2008;118:2452–2483.

Adrie C, Adib-Conquy M, Laurent I, Monchi M, Vinsonneau C, Fitting C, et al. Successful cardiopulmonary resuscitation after cardiac arrest as a "sepsis-like" syndrome. Circulation 2002;106:562–568.

Adrie C, Laurent I, Monchi M, Cariou A, Dhainaou JF, Spaulding C. Postresuscitation disease after cardiac arrest: a sepsis-like syndrome? Curr Opin Crit Care 2004;10:208–212.

Gu W, Li CS, Yin WP, Hou XM, Zhang J, Zhang D, et al. Expression imbalance of transcription factors GATA-3 and T-bet in post-resuscitation myocardial immune dysfunction in a porcine model of cardiac arrest. Resuscitation 2013;84:848–853.

Gu W, Li C, Yin W, Hou X, Zhang D. Effects of Shen-Fu Injection on the expression of T-cell specific transcription factors T-bet/GATA-3 in porcine postresuscitation lung injury. Evid Based Complement Alternat Med 2013;2013:464650.

Condotta SA, Cabrera-Perez J, Badovinac VP, Griffith TS. T-cell-mediated immunity and the role of TRAIL in sepsis-induced immunosuppression. Crit Rev Immunol 2013;33:23–40.

Okeke EB, Okwor I, Mou Z, Jia P, Uzonna JE. CD4+CD25+ regulatory T cells attenuate lipopolysaccharide-induced systemic inflammatory responses and promotes survival in murine Escherichia coli infection. Shock 2013;40:65–73.

Ning YP, Qiu ZL, Mu L. Effect of Shenfu Injection on peripheral T-lymphoeyte subsets and natural killer cell in patients with severe sepsis. Chin Arch Tradit Chin Med (Chin) 2013;31:2270–2272.

Wang J, Qiao LF, Yang GT. Role of Shenfu Injection in rats with systemic inflammatory response syndrome. Chin J Integr Med 2008;14:1–55.

Ai YH, Peng L, Zhang LN. Protective effect of Shenfu Injection on endotoxin induced acute lung injury. Chin J Crit Care Med (Chin) 2006;26:285–286.

Gu W, Li C, Yin W, Hou X. Effects of Shenfu Injection on the expression of transcription factors T-bet/GATA-3 in pigs with post-resuscitation myocardial dysfunction. Chin J Crit Care Med (Chin) 2015;27:190–196.

Gu W, Zhang Q, Yin W, Li C. Caspase-3-mediated splenic lymphocyte apoptosis in a porcine model of cardiac arrest. Am J Emerg Med 2014;32:1027–1032.

Gu W, Li C, Yin W, Guo Z, Hou X, Zhang D. Shen-Fu Injection reduces postresuscitation myocardial dysfunction in a porcine model of cardiac arrest by modulating apoptosis. Shock 2012;38:301–306.

Guo ZJ, Li CS. Therapeutic effects of Shenfu Injection on post-cardiac arrest syndrome. Chin J Integr Med 2013;19:716–720.

Zhang Y, Li CS, Wu CJ. Neuroprotective effect of Shenfu Injection following cardiac arrest in pig correlates with improved mitochondrial function and cerebral glucose uptake. Chin J Integr Med 2014;20:835–843.

Guo ZJ, Wu CJ, Li CS. Shen-fu Injection alleviates postresuscitation myocardial dysfunction by up-regulating expression of sarcoplasmic reticulum Ca2+-ATPase. Chin J Integr Med 2016;22:503–509.

Chen XW, FU J, Ruan XM. Effects of Shenfu Injection on immune function in patients during perioperative cardiopulmonary bypass. Chin J Integr Tradit West Med (Chin) 2006;26:775–779.

Qiu ZL, Ye YP, Zhang N. Clinical efficacy of Shenfu Injection in treating severe sepsis and its effects on serum levels of interleukin-6 and interleukin-10. Chin J Integr Tradit West Med (Chin) 2012;32:348–351.

Nascimento DC, Alves-Filho JC, Sônego F, Fukada SY, Pereira MS, Benjamim C, et al. Role of regulatory T cells in long-term immune dysfunction associated with severe sepsis. Crit Care Med 2010;38:1718–1725.

Wisnoski N, Chung CS, Chen Y, Huang X, Ayala A. The contribution of CD4+ CD25+ T-regulatory-cells to immune suppression in sepsis. Shock 2007;27:251–257.

Li Z, Li D, Tsun A, Li B. FOXP3+ regulatory T cells and their functional regulation. Cell Mol Immunol 2015;12:558–565.

Lu Y, Guo Y, Xu L, Li Y, Cao L. Foxp3 regulates ratio of Treg and NKT cells in a mouse model of asthma. Mol Cell Biochem 2015;403:25–31.

Abbas AK, Murphy KM, Sher A. Functional diversity of helper T lymphocytes. Nature 1996;383:787–793.

Romagnani S. Type 1 T helper and type 2 T helper cells: functions, regulation and role in protection and disease. Int J Clin Lab Res 1991;21:152–158.

Tan M, Zhu JC, Du J, Zhang LM, Yin HH. Effects of probiotics on serum levels of Th1/Th2 cytokine and clinical outcomes in severe traumatic brain-injured patients: a prospective randomized pilot study. Crit Care 2011;15:R290.

Carson WF, Ito T, Schaller M, Cavassani KA, Chensue SW, Kunkel SL. Dysregulated cytokine expression by CD4+ T cells from post-septic mice modulates both Th1 and Th2-mediated granulomatous lung inflammation. PLoS One 2011;6:e20385.

Tian X, Gou DM, Cai HJ. Effect of Shenfu Injection on the erythrocyte immune function of patients undergoing cardiopulmonary bypass. Chin J Integr Tradit West Med (Chin) 2011;31:1471–1473.

Zhang Q, Li CS, Wang S, Gu W. Effects of Chinese medicine Shen-Fu Injection on the expression of inflammatory cytokines and complements during postresuscitation immune dysfunction in a porcine model. Chin J Integr Med 2016;22:101–109.

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