Effect of Wenhua Juanbi Recipe (温化蠲痹方) on expression of receptor activator of nuclear factor kappa B ligand, osteoprotegerin, and tumor necrosis factor receptor superfamily member 14 in rats with collagen-induced arthritis

Xi-de Liu; Yun-qing Wang; Long Cai; Li-hong Ye; Fang Wang; Ying-ying Feng

doi:10.1007/s11655-016-2532-z

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Effect of Wenhua Juanbi Recipe (温化蠲痹方) on expression of receptor activator of nuclear factor kappa B ligand, osteoprotegerin, and tumor necrosis factor receptor superfamily member 14 in rats with collagen-induced arthritis

OriginalPaper | Updated：2021-08-27

- Effect of Wenhua Juanbi Recipe (温化蠲痹方) on expression of receptor activator of nuclear factor kappa B ligand, osteoprotegerin, and tumor necrosis factor receptor superfamily member 14 in rats with collagen-induced arthritis
- Effect of Wenhua Juanbi Recipe (温化蠲痹方) on expression of receptor activator of nuclear factor kappa B ligand, osteoprotegerin, and tumor necrosis factor receptor superfamily member 14 in rats with collagen-induced arthritis
- 中国结合医学杂志（英文版） 2017年23卷第3期页码：208-214
- Affiliations：
  
  1. Department of Arthropathy, Zhejiang Provincial Hospital of Integrated Traditional and Western Medicine,Hangzhou,China
  2. Zhejiang University of Traditional Chinese Medicine,Hangzhou,China
- Author bio：
- Funds：
  
  Supported by the Zhejiang Provincial Natural Science Foundation of China (No. LY12H29008);the Zhejiang Provincial Project of Traditional Chinese Medicine Science and Technology Plan (No. 2008CA086, 2009CB195, 2012ZB121, 2015ZA143);the Hangzhou Health Science and Technology Plan (No. 2010B027, No. 2014A37);the Hangzhou Social Development Plan (No. 20120633B12, No. 20160533B45)
- DOI：10.1007/s11655-016-2532-z
  中图分类号：
- 纸质出版日期：2017，
  
  网络出版日期：2016-12-21，
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Liu, Xd., Wang, Yq., Cai, L. et al. Effect of Wenhua Juanbi Recipe (温化蠲痹方) on expression of receptor activator of nuclear factor kappa B ligand, osteoprotegerin, and tumor necrosis factor receptor superfamily member 14 in rats with collagen-induced arthritis., Chin. J. Integr. Med. 23, 208–214 (2017). https://doi.org/10.1007/s11655-016-2532-z

Xi-de Liu, Yun-qing Wang, Long Cai, et al. Effect of Wenhua Juanbi Recipe (温化蠲痹方) on expression of receptor activator of nuclear factor kappa B ligand, osteoprotegerin, and tumor necrosis factor receptor superfamily member 14 in rats with collagen-induced arthritis[J]. Chinese Journal of Integrative Medicine, 2017,23(3):208-214.
Liu, Xd., Wang, Yq., Cai, L. et al. Effect of Wenhua Juanbi Recipe (温化蠲痹方) on expression of receptor activator of nuclear factor kappa B ligand, osteoprotegerin, and tumor necrosis factor receptor superfamily member 14 in rats with collagen-induced arthritis., Chin. J. Integr. Med. 23, 208–214 (2017). https://doi.org/10.1007/s11655-016-2532-z DOI：

Xi-de Liu, Yun-qing Wang, Long Cai, et al. Effect of Wenhua Juanbi Recipe (温化蠲痹方) on expression of receptor activator of nuclear factor kappa B ligand, osteoprotegerin, and tumor necrosis factor receptor superfamily member 14 in rats with collagen-induced arthritis[J]. Chinese Journal of Integrative Medicine, 2017,23(3):208-214. DOI： 10.1007/s11655-016-2532-z.

摘要

To study the effect of Wenhua Juanbi Recipe (温化蠲痹方

WJR) on expression of receptor activator of nuclear factor kappa B ligand (RANKL)

osteoprotegerin (OPG)

and tumor necrosis factor receptor superfamily member 14 (TNFRSF14

also known as LIGHT) in rats with collagen-induced arthritis (CIA). CIA rats were generated by subcutaneous injection of bovine collagen type-II at the tail base. Sixty CIA rats were randomly assigned (10 animals/group) to: model

methotrexate (MTX)-treated (0.78 mg/kg body weight)

and WJR-treated (22.9 g/kg) groups. Healthy normal rats (n=10) were used as the normal control. Treatments or saline were administered once daily by oral gavage. Rats were sacrifificed at day 28 post-treatment and knee synovium and peripheral blood serum were collected. Toe swelling degree and expression of RANKL

OPG

and LIGHT were determined by Western blot and immunohistochemistry. Compared with the normal group

toe swelling degree was signifificantly increased in the model group (P<0.01). After treatment

toe swelling degree decreased signifificantly in the WJR and MTX groups compared with the model group (P<0.01). Compared with the normal group

expression of RANKL and LIGHT were signifificantly increased and OPG signifificantly decreased in peripheral blood and synovium of the model group (P<0.01). Conversely

RANKL and LIGHT expression were signifificantly reduced and OPG increased in the WJR and MTX groups compared with the model group (P<0.01). No statistically significant difference existed between WJR and MTX groups. WJR likely acts by reducing RANKL expression and increasing OPG expression

thus inhibiting RANKL/RANK interaction and reducing LIGHT expression

thereby inhibiting osteoclast formation/activation to block bone erosion.

Abstract

To study the effect of Wenhua Juanbi Recipe (温化蠲痹方

WJR) on expression of receptor activator of nuclear factor kappa B ligand (RANKL)

osteoprotegerin (OPG)

and tumor necrosis factor receptor superfamily member 14 (TNFRSF14

methotrexate (MTX)-treated (0.78 mg/kg body weight)

OPG

and LIGHT were determined by Western blot and immunohistochemistry. Compared with the normal group

toe swelling degree was signifificantly increased in the model group (P<0.01). After treatment

toe swelling degree decreased signifificantly in the WJR and MTX groups compared with the model group (P<0.01). Compared with the normal group

expression of RANKL and LIGHT were signifificantly increased and OPG signifificantly decreased in peripheral blood and synovium of the model group (P<0.01). Conversely

thus inhibiting RANKL/RANK interaction and reducing LIGHT expression

thereby inhibiting osteoclast formation/activation to block bone erosion.

关键词

Wenhua Juanbi Recipecollagen-induced arthritisreceptor activator of nuclear factor kappa B ligandosteoprotegerintumor necrosis factor receptor superfamily member 14synoviumperipheral bloodChinese Medicine

Keywords

references

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