Contribution of Borneolum syntheticum to the Intervention Effect of Liuwei Dihuang Pill (六味地黄丸) on Experimental Retinal Degeneration

Li-na Liang; Yu-yang Bai; You-zhi Tang; Qiang Chen; Xue-li Li; Qun-ying Ma; Jie Liang; Jiao Li

doi:10.1007/s11655-016-2584-0

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Contribution of Borneolum syntheticum to the Intervention Effect of Liuwei Dihuang Pill (六味地黄丸) on Experimental Retinal Degeneration

OriginalPaper | Updated：2021-08-27

- Contribution of Borneolum syntheticum to the Intervention Effect of Liuwei Dihuang Pill (六味地黄丸) on Experimental Retinal Degeneration
- Contribution of Borneolum syntheticum to the Intervention Effect of Liuwei Dihuang Pill (六味地黄丸) on Experimental Retinal Degeneration
- 中国结合医学杂志（英文版） 2018年24卷第6期页码：442-447
- Affiliations：
  
  Ophthalmology Research Laboratory, Eye Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-016-2584-0
  中图分类号：
- 纸质出版日期：2018，
  
  网络出版日期：2016-5-12，
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Liang, Ln., Bai, Yy., Tang, Yz. et al. Contribution of Borneolum syntheticum to the Intervention Effect of Liuwei Dihuang Pill (六味地黄丸) on Experimental Retinal Degeneration., Chin. J. Integr. Med. 24, 442–447 (2018). https://doi.org/10.1007/s11655-016-2584-0

Li-na Liang, Yu-yang Bai, You-zhi Tang, et al. Contribution of Borneolum syntheticum to the Intervention Effect of Liuwei Dihuang Pill (六味地黄丸) on Experimental Retinal Degeneration[J]. Chinese Journal of Integrative Medicine, 2018,24(6):442-447.
Liang, Ln., Bai, Yy., Tang, Yz. et al. Contribution of Borneolum syntheticum to the Intervention Effect of Liuwei Dihuang Pill (六味地黄丸) on Experimental Retinal Degeneration., Chin. J. Integr. Med. 24, 442–447 (2018). https://doi.org/10.1007/s11655-016-2584-0 DOI：

Li-na Liang, Yu-yang Bai, You-zhi Tang, et al. Contribution of Borneolum syntheticum to the Intervention Effect of Liuwei Dihuang Pill (六味地黄丸) on Experimental Retinal Degeneration[J]. Chinese Journal of Integrative Medicine, 2018,24(6):442-447. DOI： 10.1007/s11655-016-2584-0.

摘要

To observe the contribution of Borneolum syntheticum to the intervention effect of Liuwei Dihuang Pill (六味地黄丸

LDP) on experimental retinal degeneration

and initially investigate the mechanism of Borneolum syntheticum as meridian-lead-in drug. A total of 180 sodium iodateinduced retinital degeneration rats were randomly divided into three groups

including distilled water group

LDP group

and LDP+Borneolum syntheticum (LDP+BS) group. Twenty normal rats were fed regularly without any treatment as normal control. On day 7 and 14 after treatment

histopathological study and transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL) test were performed to evaluate the retinopathy. Claudin-5 expression at blood-retina barrier (BRB) was detected by Western blot at different time points from 0.5 to 8 h after gavage. On day 7 and 14 after treatment

the retinal lesion grades were significantly different among the three groups (P<0.05). The grade in the LDP+BS group was significantly less than the LDP and distilled water groups (both P<0.05)

no significant difference was observed between the LDP and distilled water groups (P>0.05). The apoptosis rates in the LDP+BS group was significantly less than the distilled water and LDP groups (both P<0.05)

while there was no significant difference between LDP and distilled water groups (P>0.05). Expression of claudin-5 in LDP+BS group was significantly less than the other two groups at 0.5

1 and 2 h after gavage (P<0.05). There was no apparent difference among the three groups at 4 and 8 h after gavage (P>0.05). Borneolum syntheticum could strengthen the effect of LDP on experimental retinal degeneration

indicated that Borneolum syntheticum might play the role of meridian-lead-in drug in the formula. The mechanism may be due to Borneolum syntheticum could promote the physiologically openness of bloodretina barrier through transiently affecting the expression of claudin-5.

Abstract

To observe the contribution of Borneolum syntheticum to the intervention effect of Liuwei Dihuang Pill (六味地黄丸

LDP) on experimental retinal degeneration

and initially investigate the mechanism of Borneolum syntheticum as meridian-lead-in drug. A total of 180 sodium iodateinduced retinital degeneration rats were randomly divided into three groups

including distilled water group

LDP group

and LDP+Borneolum syntheticum (LDP+BS) group. Twenty normal rats were fed regularly without any treatment as normal control. On day 7 and 14 after treatment

the retinal lesion grades were significantly different among the three groups (P<0.05). The grade in the LDP+BS group was significantly less than the LDP and distilled water groups (both P<0.05)

while there was no significant difference between LDP and distilled water groups (P>0.05). Expression of claudin-5 in LDP+BS group was significantly less than the other two groups at 0.5

关键词

meridian-lead-in drugBorneolum syntheticumLiuwei Dihuang PillRetinal Degeneration

Keywords

meridian-lead-in drugBorneolum syntheticumLiuwei Dihuang PillRetinal Degeneration

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