<italic style="font-style: italic">Prunella vulgaris</italic> Polysaccharide Inhibits Growth and Migration of Breast Carcinoma-Associated Fibroblasts by Suppressing Expression of Basic Fibroblast Growth Factor

Jian HAO; Xiu-li DING; Xue YANG; Xiong-zhi WU

doi:10.1007/s11655-016-2587-x

Your Location：

Home >

Browse articles >

Prunella vulgaris Polysaccharide Inhibits Growth and Migration of Breast Carcinoma-Associated Fibroblasts by Suppressing Expression of Basic Fibroblast Growth Factor

Original Article | Updated：2021-08-23

- Prunella vulgaris Polysaccharide Inhibits Growth and Migration of Breast Carcinoma-Associated Fibroblasts by Suppressing Expression of Basic Fibroblast Growth Factor
- Prunella vulgaris Polysaccharide Inhibits Growth and Migration of Breast Carcinoma-Associated Fibroblasts by Suppressing Expression of Basic Fibroblast Growth Factor
- Chinese Journal of Integrative Medicine 2020年26卷第4期页码：270-276
- Affiliations：
  
  1.Zhong-Shan-Men In-Patient Department, Tianjin Medical University Cancer Institute and Hospital; National Clinical Research Center for Cancer; Key Laboratory of Cancer Prevention and Therapy, Tianjin (300060), China
  2.Clincal Center of Tumor Therapy, The Fourth Central Hospital Affiliated to Nankai University, Tianjin (300140), China
- Author bio：
  
  Correspondence to: Prof. WU Xiong-zhi, E-mail:wuxiongzhi@163.com
- Funds：
  
  the National Science Foundation of China(81173376);New Century Excellent Talent(NCET-11-1068)
- DOI：10.1007/s11655-016-2587-x
  中图分类号：
- 纸质出版日期：2020-04-01，
  
  网络出版日期：2016-09-01，
  
  录用日期：2015-06-19
Scan for full text
Jian HAO, Xiu-li DING, Xue YANG, 等. Prunella vulgaris Polysaccharide Inhibits Growth and Migration of Breast Carcinoma-Associated Fibroblasts by Suppressing Expression of Basic Fibroblast Growth Factor[J]. Chinese Journal of Integrative Medicine, 2020,26(4):270-276.

Jian HAO, Xiu-li DING, Xue YANG, et al. Prunella vulgaris Polysaccharide Inhibits Growth and Migration of Breast Carcinoma-Associated Fibroblasts by Suppressing Expression of Basic Fibroblast Growth Factor[J]. Chinese Journal of Integrative Medicine, 2020,26(4):270-276.
Jian HAO, Xiu-li DING, Xue YANG, 等. Prunella vulgaris Polysaccharide Inhibits Growth and Migration of Breast Carcinoma-Associated Fibroblasts by Suppressing Expression of Basic Fibroblast Growth Factor[J]. Chinese Journal of Integrative Medicine, 2020,26(4):270-276. DOI： 10.1007/s11655-016-2587-x.

Jian HAO, Xiu-li DING, Xue YANG, et al. Prunella vulgaris Polysaccharide Inhibits Growth and Migration of Breast Carcinoma-Associated Fibroblasts by Suppressing Expression of Basic Fibroblast Growth Factor[J]. Chinese Journal of Integrative Medicine, 2020,26(4):270-276. DOI： 10.1007/s11655-016-2587-x.

摘要

Abstract

Objective:

To study the effects of

Prunella vulgaris polysaccharide

(PVP) on human breast carcinoma-associated fibroblasts (CAFs).

Method:

Cell viability was detected by 3-[4

5-dimethylthiazol-2-yl]-2

5-(3-carboxymethoxyphenyl)-2-4-sulfophenyl)-2H-tetrazolium (MTS) assay. Wound healing experiment and transwell migration assay were used to investigate the anti-migration effects. Flow cytometry was applied to detect cell apoptosis and cell cycle distribution. Reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay were used to detect the expression of basic fibroblast growth factor (bFGF) in CAFs. Culture SKBr-3 with CAFs conditioned medium (CAFs-CM) to evaluate the indirect function on the proliferation of breast cancer SKBr-3 cells.

Results:

PVP inhibited the viability of CAFs by inducing apoptosis (

0.01) and arresting cell cycle (

0.01). It also inhibited the migration of CAFs (

0.01). bFGF promoted CAFs proliferation (

0.01) and migration (

0.01)

protected CAFs from apoptosis (

0.05) and reduced G0 phase to 49.06% (

0.01). However

these effects of bFGF on CAFs could be abrogated by PVP. Culturing SKBr-3 with CAFs-CM

PVP could inhibit the viability of breast cancer SKBr-3 cells indirectly. Moreover

PVP reduced the mRNA expression (

0.01) and protein secretion of bFGF (

0.01) in CAFs.

Conclusion:

PVP could exert an anti-cancer effect on breast CAFs by inhibiting bFGF expression

thus inhibiting the growth of breast cancer SKBr-3 cells indirectly.

关键词

Keywords

Prunella vulgaris polysaccharidecarcinoma-associated fibroblastsbasic fibroblast growth factorSKBr-3Chinese medicine

references

More>

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Chemical Composition, Pharmacological Effects and Clinical Applications of Cinobufacini

Immunomodulatory Function of Pien Tze Huang in T Cell-Mediated Anti-tumor Activity against B16-F10, MC38 and Hep1-6 Tumor Models

Safflower Yellow Inhibits Progression of Hepatocellular Carcinoma by Modulating Immunological Tolerance via FAK Pathway

TRAIL and Celastrol Combinational Treatment Suppresses Proliferation, Migration, and Invasion of Human Glioblastoma Cells via Targeting Wnt/β-Catenin Signaling Pathway

Immunoregulatory and Anti-cancer Activities of Combination Treatment of Novel Four-Herb Formula and Doxorubicin in 4T1-Breast Cancer Bearing Mice

Prunella vulgaris Polysaccharide Inhibits Growth and Migration of Breast Carcinoma-Associated Fibroblasts by Suppressing Expression of Basic Fibroblast Growth Factor

Prunella vulgaris Polysaccharide Inhibits Growth and Migration of Breast Carcinoma-Associated Fibroblasts by Suppressing Expression of Basic Fibroblast Growth Factor

DOI：10.1007/s11655-016-2587-x

摘要

Abstract

关键词

Keywords

references