Pure total flavonoids from Citrus paradisi Macfad induce leukemia cell apoptosis in vitro

Tie-ying Dai; Bo Wang; Sheng-yun Lin; Jian-ping Jiang; Li-qiang Wu; Wen-bing Qian

doi:10.1007/s11655-016-2593-z

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Pure total flavonoids from Citrus paradisi Macfad induce leukemia cell apoptosis in vitro

OriginalPaper | Updated：2021-08-27

- Pure total flavonoids from Citrus paradisi Macfad induce leukemia cell apoptosis in vitro
- Pure total flavonoids from Citrus paradisi Macfad induce leukemia cell apoptosis in vitro
- 中国结合医学杂志（英文版） 2017年23卷第5期页码：370-375
- Affiliations：
  
  1. Department of Hematology, the First Hospital Affiliated to Zhejiang University of Traditional Chinese Medicine,Hangzhou,China
  2. Department of Hematology, the First Affiliated Hospital of Medical School of Zhejiang University,Hangzhou,China
- Author bio：
- Funds：
  
  Supported by Zhejiang Provincial Natural Science Foundation of China (No. LY14H080003)
- DOI：10.1007/s11655-016-2593-z
  中图分类号：
- 纸质出版日期：2017，
  
  网络出版日期：2016-7-28，
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Dai, Ty., Wang, B., Lin, Sy. et al. Pure total flavonoids from Citrus paradisi Macfad induce leukemia cell apoptosis in vitro, ., Chin. J. Integr. Med. 23, 370–375 (2017). https://doi.org/10.1007/s11655-016-2593-z

Tie-ying Dai, Bo Wang, Sheng-yun Lin, et al. Pure total flavonoids from Citrus paradisi Macfad induce leukemia cell apoptosis in vitro[J]. Chinese Journal of Integrative Medicine, 2017,23(5):370-375.
Dai, Ty., Wang, B., Lin, Sy. et al. Pure total flavonoids from Citrus paradisi Macfad induce leukemia cell apoptosis in vitro, ., Chin. J. Integr. Med. 23, 370–375 (2017). https://doi.org/10.1007/s11655-016-2593-z DOI：

Tie-ying Dai, Bo Wang, Sheng-yun Lin, et al. Pure total flavonoids from Citrus paradisi Macfad induce leukemia cell apoptosis in vitro[J]. Chinese Journal of Integrative Medicine, 2017,23(5):370-375. DOI： 10.1007/s11655-016-2593-z.

摘要

To investigate the potential effect of pure total flavonoids from Citrus paradisi Macfad peel (PTFC) on the proliferation of human myeloid leukemia cells Kasumi-1

HL-60 and K562

and the underlying mechanisms. PTFC was extracted from Citrus paradisi Macfad peel and was identified by high performance liquid chromatography. The effect of PTFC on the proliferation and apoptosis of leukemia cells were determined by 3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide

fluorescent microscopy and flow cytometry

respectively. The effect of PTFC on the expression levels of apoptosis-related regulators was determined by Western blot assay. Treatment with PTFC inhibited leukemia cell proliferation in a dose- and time-dependent manner and triggered Kasumi-1 cell apoptosis. Treatment with PTFC significantly increased the levels of activated poly adenosine diphosphate-ribosepolymerase and caspase-3/-9

but reduced the levels of Mcl-1 expression in Kasumi-1 cells. However

PTFC did not obviously induce HL-60 cell apoptosis. PTFC inhibited leukemia cell proliferation and induced their apoptosis by modulating apoptosisrelated regulator expression in leukemia cells in vitro.

Abstract

To investigate the potential effect of pure total flavonoids from Citrus paradisi Macfad peel (PTFC) on the proliferation of human myeloid leukemia cells Kasumi-1

HL-60 and K562

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide

fluorescent microscopy and flow cytometry

but reduced the levels of Mcl-1 expression in Kasumi-1 cells. However

关键词

total flavonoids from Citrus paradisi Macfadhuman myeloid leukemia cellsgrowth inhibitionapoptosisChinese Medicine

Keywords

total flavonoids from Citrus paradisi Macfadhuman myeloid leukemia cellsgrowth inhibitionapoptosisChinese Medicine

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