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Jiangtang Xiaozhi Recipe (降糖消脂方) prevents diabetic retinopathy in streptozotocin-induced diabetic rats
Jiangtang Xiaozhi Recipe (降糖消脂方) prevents diabetic retinopathy in streptozotocin-induced diabetic rats
- 中国结合医学杂志（英文版） 2017年23卷第6期 页码：425-432
- Affiliations：
1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China
2. Experiment Research Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：Supported by the National Science and Technology Major Project of China (No. 2010ZX09102-213), Beijing Natural Science Foundation, China (No. 7152100), and Program for Innovative Research Team in the Institute of Medicinal Plant Development, China (No. IT 1305)
DOI：10.1007/s11655-016-2595-x
中图分类号：纸质出版日期：2017，
网络出版日期：2016-6-23，
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Li, L., Li, Yl., Zhou, Yf. et al. Jiangtang Xiaozhi Recipe (降糖消脂方) prevents diabetic retinopathy in streptozotocin-induced diabetic rats., Chin. J. Integr. Med. 23, 425–432 (2017). https://doi.org/10.1007/s11655-016-2595-x
Lin Li, Yan-lin Li, Yun-feng Zhou, et al. Jiangtang Xiaozhi Recipe (降糖消脂方) prevents diabetic retinopathy in streptozotocin-induced diabetic rats[J]. Chinese Journal of Integrative Medicine, 2017,23(6):425-432.
Li, L., Li, Yl., Zhou, Yf. et al. Jiangtang Xiaozhi Recipe (降糖消脂方) prevents diabetic retinopathy in streptozotocin-induced diabetic rats., Chin. J. Integr. Med. 23, 425–432 (2017). https://doi.org/10.1007/s11655-016-2595-x DOI：
Lin Li, Yan-lin Li, Yun-feng Zhou, et al. Jiangtang Xiaozhi Recipe (降糖消脂方) prevents diabetic retinopathy in streptozotocin-induced diabetic rats[J]. Chinese Journal of Integrative Medicine, 2017,23(6):425-432. DOI： 10.1007/s11655-016-2595-x.
摘要
To evaluate the prevention effect of diabetic retinopathy of Jiangtang Xiaozhi Recipe (降糖消脂方
JXR) in streptozotocin (STZ)-induced diabetic rats. Sprague-Dawley rats were randomly divided into normal control group and diabetic group. Rats in the diabetic group were induced by intraperitoneal administration of STZ (50 mg/kg)
and subdivided into 5 groups. Rats in the diabetic control group were given saline; four treatment groups were given metformin (300 mg/kg)
JXR (2
4 and 8 g/kg) respectively for 8 weeks
while rats in the normal control group were injected with citrate buffer and given the same volume of vehicle. Body weight and food intake were measured every week. The hypoglycaemic effects were determined by testing fasting blood glucose (FBG) every other week
and hemoglobin A1c (HbA1c)
insulin
and glucagon at the end of the treatment. The preventive effects of JXR on STZ-induced diabetic rats were determined by histopathological examination with hematoxylin and eosin staining
and periodic acid-schiff staining. The effects were further evaluated by serum superoxide dismutase (SOD) activity and malondialdehyde (MDA). High-dose JXR significantly reduced FBG and HbA1c level at the 8th week of administration (P<0.01
P<0.05). JXR significantly increased insulin level (P<0.05)
and decreased glucagon level (P<0.05). JXR showed the antioxidant defense with increased SOD activity and decreased MDA contents in diabetic rats. Histopathological studies revealed that there were no basement membrane thickening and mild destruction in the treated groups. Morphometric measurements of retina microvascular showed that acellular capillary and capillary density decreased in treated rats while pericyte and endothelial cell increasing after the treatment. JXR have protective effect of diabetic retinopathy and its mechanism may be associated with the obvious hypoglycemic and antioxidant effect.
Abstract
To evaluate the prevention effect of diabetic retinopathy of Jiangtang Xiaozhi Recipe (降糖消脂方
JXR) in streptozotocin (STZ)-induced diabetic rats. Sprague-Dawley rats were randomly divided into normal control group and diabetic group. Rats in the diabetic group were induced by intraperitoneal administration of STZ (50 mg/kg)
and subdivided into 5 groups. Rats in the diabetic control group were given saline; four treatment groups were given metformin (300 mg/kg)
JXR (2
4 and 8 g/kg) respectively for 8 weeks
while rats in the normal control group were injected with citrate buffer and given the same volume of vehicle. Body weight and food intake were measured every week. The hypoglycaemic effects were determined by testing fasting blood glucose (FBG) every other week
and hemoglobin A1c (HbA1c)
insulin
and glucagon at the end of the treatment. The preventive effects of JXR on STZ-induced diabetic rats were determined by histopathological examination with hematoxylin and eosin staining
and periodic acid-schiff staining. The effects were further evaluated by serum superoxide dismutase (SOD) activity and malondialdehyde (MDA). High-dose JXR significantly reduced FBG and HbA1c level at the 8th week of administration (P<0.01
P<0.05). JXR significantly increased insulin level (P<0.05)
and decreased glucagon level (P<0.05). JXR showed the antioxidant defense with increased SOD activity and decreased MDA contents in diabetic rats. Histopathological studies revealed that there were no basement membrane thickening and mild destruction in the treated groups. Morphometric measurements of retina microvascular showed that acellular capillary and capillary density decreased in treated rats while pericyte and endothelial cell increasing after the treatment. JXR have protective effect of diabetic retinopathy and its mechanism may be associated with the obvious hypoglycemic and antioxidant effect.
关键词
Jiangtang Xiaozhi RecipeChinese Medicinestreptozotocindiabetes mellitusdiabetic retinopathy
Keywords
Jiangtang Xiaozhi RecipeChinese Medicinestreptozotocindiabetes mellitusdiabetic retinopathy
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