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Identification of human serum protein targets of Qianggu Decoction (强骨饮) in primary type I osteoporosis based on tandem mass tag labeling and liquid chromatography-tandem mass spectrometry technology
Identification of human serum protein targets of Qianggu Decoction (强骨饮) in primary type I osteoporosis based on tandem mass tag labeling and liquid chromatography-tandem mass spectrometry technology
- 中国结合医学杂志（英文版） 2017年23卷第10期 页码：747-754
- Affiliations：
1. The Second Clinical Medical College, Zhejiang Chinese Medical University,Hangzhou,China
2. Department of Ortopaedics and Traumatology, the Second Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou,China
3. Department of Diagnostics of Traditional Chinese Medicine, College of Basic Medical Science, Zhejiang Chinese Medical University,Hangzhou,China
- Author bio：
- Funds：Supported by the National Natural Science Foundation of China (No. 81373878) and Natural Science Foundation of Zhejiang Province, China (No. LY13H290009 and No. LY12H29007)
DOI：10.1007/s11655-016-2600-4
中图分类号：纸质出版日期：2017，
网络出版日期：2016-7-7，
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Liang, Bc., Shi, Xl., Li, Cw. et al. Identification of human serum protein targets of Qianggu Decoction (强骨饮) in primary type I osteoporosis based on tandem mass tag labeling and liquid chromatography-tandem mass spectrometry technology., Chin. J. Integr. Med. 23, 747–754 (2017). https://doi.org/10.1007/s11655-016-2600-4
Bo-cheng Liang, Xiao-lin Shi, Chun-wen Li, et al. Identification of human serum protein targets of Qianggu Decoction (强骨饮) in primary type I osteoporosis based on tandem mass tag labeling and liquid chromatography-tandem mass spectrometry technology[J]. Chinese Journal of Integrative Medicine, 2017,23(10):747-754.
Liang, Bc., Shi, Xl., Li, Cw. et al. Identification of human serum protein targets of Qianggu Decoction (强骨饮) in primary type I osteoporosis based on tandem mass tag labeling and liquid chromatography-tandem mass spectrometry technology., Chin. J. Integr. Med. 23, 747–754 (2017). https://doi.org/10.1007/s11655-016-2600-4 DOI：
Bo-cheng Liang, Xiao-lin Shi, Chun-wen Li, et al. Identification of human serum protein targets of Qianggu Decoction (强骨饮) in primary type I osteoporosis based on tandem mass tag labeling and liquid chromatography-tandem mass spectrometry technology[J]. Chinese Journal of Integrative Medicine, 2017,23(10):747-754. DOI： 10.1007/s11655-016-2600-4.
摘要
To investigate the serum protein targets of Qianggu Decoction (强骨饮
QGD) on treating osteoporosis by the proteomics analysis using tandem mass tag (TMT) and liquid chromatographytandem mass spectrometry (LC-MS/MS). Twenty serum protein samples were recruited (10 patients with primary type I osteoporosis before and after QGD treatment) and the high abundance ratios protein was removed
two serum samples were extracted and labeled with TMT reagent. Then
mass spectrometric detection
identification of differentially expressed proteins and bioinformatics analysis of differentially expressed proteins were carried out. A total of 60 proteins were identified
within a 99% confidence interval
to be differentially regulated of which
34 proteins were up-regulated and 26 proteins were down-regulated. Differentially expressed proteins analyzed by Gene Ontology (GO) annotation mainly get involved in 12 different biological processes
7 types of cellular components
and 6 kinds of molecular functions. Angiotensinogen (AGT)
stromelysin-1 (MMP3)
heparanase (HPSE) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were screened as candidate protein targets of QGD treatment
which were related to metabolic mechanism of bone remodeling and/or bone collagen of osteoporosis. By the utilization of the protein-protein interaction network analysis tool named STRING10.0
it showed that AGT
MMP3
HPSE and GAPDH were located in the key node of the protein-protein interactions network. Furthermore
AGT
MMP3
HPSE and GAPDH were found to be directly related to BMP
MAPK
Wnt
SMAD and tumor necrosis factor ligand superfamily member 11 (TNFSF11) families. The proteomics analysis by using TMT combined with LC-MS/MS was a feasible method for screening the potential therapeutic targets associated with QGD treatment. It suggests that AGT
MMP3
HPSE and GAPDH may be candidate protein targets of QGD treatment which can be used as therapeutic effect monitor and early diagnosis of primary type I osteoporosis.
Abstract
To investigate the serum protein targets of Qianggu Decoction (强骨饮
QGD) on treating osteoporosis by the proteomics analysis using tandem mass tag (TMT) and liquid chromatographytandem mass spectrometry (LC-MS/MS). Twenty serum protein samples were recruited (10 patients with primary type I osteoporosis before and after QGD treatment) and the high abundance ratios protein was removed
two serum samples were extracted and labeled with TMT reagent. Then
mass spectrometric detection
identification of differentially expressed proteins and bioinformatics analysis of differentially expressed proteins were carried out. A total of 60 proteins were identified
within a 99% confidence interval
to be differentially regulated of which
34 proteins were up-regulated and 26 proteins were down-regulated. Differentially expressed proteins analyzed by Gene Ontology (GO) annotation mainly get involved in 12 different biological processes
7 types of cellular components
and 6 kinds of molecular functions. Angiotensinogen (AGT)
stromelysin-1 (MMP3)
heparanase (HPSE) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were screened as candidate protein targets of QGD treatment
which were related to metabolic mechanism of bone remodeling and/or bone collagen of osteoporosis. By the utilization of the protein-protein interaction network analysis tool named STRING10.0
it showed that AGT
MMP3
HPSE and GAPDH were located in the key node of the protein-protein interactions network. Furthermore
AGT
MMP3
HPSE and GAPDH were found to be directly related to BMP
MAPK
Wnt
SMAD and tumor necrosis factor ligand superfamily member 11 (TNFSF11) families. The proteomics analysis by using TMT combined with LC-MS/MS was a feasible method for screening the potential therapeutic targets associated with QGD treatment. It suggests that AGT
MMP3
HPSE and GAPDH may be candidate protein targets of QGD treatment which can be used as therapeutic effect monitor and early diagnosis of primary type I osteoporosis.
关键词
primary type I osteoporosistonifying qi and warming meridiansChinese Medicineproteomics
Keywords
primary type I osteoporosistonifying qi and warming meridiansChinese Medicineproteomics
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