Therapeutic Role of Tangshenkang Granule (糖肾康颗粒) in Rat Model with Diabetic Nephropathy

Shun-jin Hu; Bing Shu; Hua Jin; Xiao-feng Li; Jia-rong Mao; Ke-jun Ren; Lei Gao; Li Yang; You-wan Wu; Yong-jun Wang

doi:10.1007/s11655-016-2607-x

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Therapeutic Role of Tangshenkang Granule (糖肾康颗粒) in Rat Model with Diabetic Nephropathy

OriginalPaper | Updated：2021-08-27

- Therapeutic Role of Tangshenkang Granule (糖肾康颗粒) in Rat Model with Diabetic Nephropathy
- Therapeutic Role of Tangshenkang Granule (糖肾康颗粒) in Rat Model with Diabetic Nephropathy
- 中国结合医学杂志（英文版） 2018年24卷第8期页码：600-605
- Affiliations：
  
  1. Department of Internal Medicine, the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine,Hefei,China
  2. Department of Orthopaedics, Longhua Hospital, Shanghai University of Traditional Chinese Medicine,Shanghai,China
  3. Spine Research Institute, Shanghai University of Traditional Chinese Medicine,Shanghai,China
  4. Department of Pathology, Shanxi Hospital of Traditional Chinese Medicine,Xi’an,China
  5. Graduate School, Anhui University of Traditional Chinese Medicine,Hefei,China
- Author bio：
- Funds：
  
  Supported by the Scientific Research Project of Colleges and Universities in Anhui Province, China (No. 2013z172) and the National Natural Science Foundation of China (No. 81503590)
- DOI：10.1007/s11655-016-2607-x
  中图分类号：
- 纸质出版日期：2018，
  
  网络出版日期：2016-10-24，
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Hu, Sj., Shu, B., Jin, H. et al. Therapeutic Role of Tangshenkang Granule (糖肾康颗粒) in Rat Model with Diabetic Nephropathy., Chin. J. Integr. Med. 24, 600–605 (2018). https://doi.org/10.1007/s11655-016-2607-x

Shun-jin Hu, Bing Shu, Hua Jin, et al. Therapeutic Role of Tangshenkang Granule (糖肾康颗粒) in Rat Model with Diabetic Nephropathy[J]. Chinese Journal of Integrative Medicine, 2018,24(8):600-605.
Hu, Sj., Shu, B., Jin, H. et al. Therapeutic Role of Tangshenkang Granule (糖肾康颗粒) in Rat Model with Diabetic Nephropathy., Chin. J. Integr. Med. 24, 600–605 (2018). https://doi.org/10.1007/s11655-016-2607-x DOI：

Shun-jin Hu, Bing Shu, Hua Jin, et al. Therapeutic Role of Tangshenkang Granule (糖肾康颗粒) in Rat Model with Diabetic Nephropathy[J]. Chinese Journal of Integrative Medicine, 2018,24(8):600-605. DOI： 10.1007/s11655-016-2607-x.

摘要

To evaluate the renal protective effect of Tangshenkang Granule (糖肾康颗粒) in a rat model of diabetic nephropathy (DN). Forty male Sprague-Dawley rats were randomly divided into control

Tangshenkang and benazepril groups. DN model was established in the rats of DN

Tangshenkang and benazepril groups. Tangshenkang Granule solution and benazepril hydrochloride solution were intragastrically administered daily to the rats in the Tangshenkang and benazepril groups for 8 weeks

respectively. Urinary albumin and creatinine were detected. The albumin/creatinine (ACR) was calculated in addition to 24 h urinary protein (24-h UPr)

serum creatinine (Scr)

blood urea nitrogen (BUN)

total cholesterol (TC)

triglyceride (TG)

low-density lipoprotein (LDL)

high-density lipoprotein (HDL)

and creatinine clearance rate (Ccr). Right kidneys were harvested for pathological observation using periodic acid-silver methenamine-Masson staining. The average glomerular diameter (DG)

average glomerular (AG) and mesangial areas (AM) were measured. The thickness of glomerular basement membrane (TGBM) was detected using transmission electron microscope. Compared with rats in the control group

rats in the DN group showed significantly decreased body weight

increased hypertrophy index

24-h urinary volume

24-h UPr

ACR

Scr

BUN

Ccr

blood lipids as well as renal pathological indices including DG

AM/AG and TGBM (P <0.05). Compared with the DN group

the weights of rats in the Tangshenkang and benazepril groups were significantly increased

and the renal hypertrophy indices were significantly decreased (P <0.05). The 24-h urinary volumes

ACR

24-h UPr

Scr

BUN

Ccr

LDL

AM and TGBM were obviously decreased (P <0.05). Compared with the benazepril group

the Tangshenkang group showed significantly decreased levels of ACR

24-h UPr

AG and AM (P <0.05). Tangshenkang Granule decreased the urinary protein

attenuated the high glomerular filtration rate and improved lipid metabolism in DN rats

and prevented further injury induced by diabetic nephropathy.

Abstract

To evaluate the renal protective effect of Tangshenkang Granule (糖肾康颗粒) in a rat model of diabetic nephropathy (DN). Forty male Sprague-Dawley rats were randomly divided into control

Tangshenkang and benazepril groups. DN model was established in the rats of DN

respectively. Urinary albumin and creatinine were detected. The albumin/creatinine (ACR) was calculated in addition to 24 h urinary protein (24-h UPr)

serum creatinine (Scr)

blood urea nitrogen (BUN)

total cholesterol (TC)

triglyceride (TG)

low-density lipoprotein (LDL)

high-density lipoprotein (HDL)

and creatinine clearance rate (Ccr). Right kidneys were harvested for pathological observation using periodic acid-silver methenamine-Masson staining. The average glomerular diameter (DG)

rats in the DN group showed significantly decreased body weight

increased hypertrophy index

24-h urinary volume

24-h UPr

ACR

Scr

BUN

Ccr

blood lipids as well as renal pathological indices including DG

AM/AG and TGBM (P <0.05). Compared with the DN group

the weights of rats in the Tangshenkang and benazepril groups were significantly increased

and the renal hypertrophy indices were significantly decreased (P <0.05). The 24-h urinary volumes

ACR

24-h UPr

Scr

BUN

Ccr

LDL

AM and TGBM were obviously decreased (P <0.05). Compared with the benazepril group

the Tangshenkang group showed significantly decreased levels of ACR

24-h UPr

AG and AM (P <0.05). Tangshenkang Granule decreased the urinary protein

attenuated the high glomerular filtration rate and improved lipid metabolism in DN rats

and prevented further injury induced by diabetic nephropathy.

关键词

diabetic nephropathyTangshenkang Granulekidney functionlipid metabolismkidney pathologyChinese Medicine

Keywords

diabetic nephropathyTangshenkang Granulekidney functionlipid metabolismkidney pathologyChinese Medicine

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