Salvianolate reduces murine myocardial ischemia and reperfusion injury via ERK1/2 signaling pathways in vivo

Jian-yong Qi; Juan Yu; Dong-hui Huang; Li-heng Guo; Lei Wang; Xin Huang; Hai-ding Huang; Miao Zhou; Min-zhou Zhang; Jiashin Wu

doi:10.1007/s11655-016-2621-z

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Salvianolate reduces murine myocardial ischemia and reperfusion injury via ERK1/2 signaling pathways in vivo

OriginalPaper | Updated：2021-08-27

- Salvianolate reduces murine myocardial ischemia and reperfusion injury via ERK1/2 signaling pathways in vivo
- Salvianolate reduces murine myocardial ischemia and reperfusion injury via ERK1/2 signaling pathways in vivo
- 中国结合医学杂志（英文版） 2017年23卷第1期页码：40-47
- Affiliations：
  
  1. Intensive Care Laboratory, Guangdong Province Hospital of Chinese Medicine, 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou,China
  2. Animal Laboratory, Guangdong Province Hospital of Chinese Medicine, 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou,China
  3. Department of Oral and Maxillary Surgery, Stomatology Hospital of Guangzhou Medical University,Guangzhou,China
  4. Northeast Ohio Medical University, Rootstown,USA
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 81473471 and No. 81573708) and Foundation of Guangdong Hospital of Chinese Medicine (No.YK2013B2N11, No. YN2014ZH01, and No. YN2014ZHR203)
- DOI：10.1007/s11655-016-2621-z
  中图分类号：
- 纸质出版日期：2017，
  
  网络出版日期：2016-10-27，
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Qi, Jy., Yu, J., Huang, Dh. et al. Salvianolate reduces murine myocardial ischemia and reperfusion injury via ERK1/2 signaling pathways in vivo, ., Chin. J. Integr. Med. 23, 40–47 (2017). https://doi.org/10.1007/s11655-016-2621-z

Jian-yong Qi, Juan Yu, Dong-hui Huang, et al. Salvianolate reduces murine myocardial ischemia and reperfusion injury via ERK1/2 signaling pathways in vivo[J]. Chinese Journal of Integrative Medicine, 2017,23(1):40-47.
Qi, Jy., Yu, J., Huang, Dh. et al. Salvianolate reduces murine myocardial ischemia and reperfusion injury via ERK1/2 signaling pathways in vivo, ., Chin. J. Integr. Med. 23, 40–47 (2017). https://doi.org/10.1007/s11655-016-2621-z DOI：

Jian-yong Qi, Juan Yu, Dong-hui Huang, et al. Salvianolate reduces murine myocardial ischemia and reperfusion injury via ERK1/2 signaling pathways in vivo[J]. Chinese Journal of Integrative Medicine, 2017,23(1):40-47. DOI： 10.1007/s11655-016-2621-z.

摘要

To analyze the effects of salvianolate on myocardial infarction in a murine in vivo model of ischemia and reperfusion (I/R) injury. Myocardial I/R injury model was constructed in mice by 30 min of coronary occlusion followed by 24 h of reperfusion and pretreated with salvianolate 30 min before I/R (SAL group). The SAL group was compared with SHAM (no I/R and no salvianolate)

I/R (no salvianolate)

and ischemia preconditioning (IPC) groups. Furthermore

an ERK1/2 inhibitor PD98059 (1 mg/kg)

and a phosphatidylinositol-3-kinase (PI3-K) inhibitor

LY294002 (7.5 mg/kg)

were administered intraperitoneal injection (i.p) for 30 min prior to salvianolate

followed by I/R surgery in LY and PD groups. By using a double staining method

the ratio of the infarct size (IS) to left ventricle (LV) and of risk region (RR) to LV were compared among the groups. Correlations between IS and RR were analyzed. Western-blot was used to detect the extracellular signal-regulated kinase 1/2 (ERK1/2) and protein kinase B (AKT) phosphorylation changes. There were no significant differences between RR to LV ratio among the SHAM

I/R

IPC and SAL groups (P>0.05). The SAL and IPC groups had IS of 26.1%±1.4% and 22.3%±2.9% of RR

respectively

both of which were significantly smaller than the I/R group (38.5%±2.9% of RR

P<0.05

P<0.01

respectively). Moreover

the phosphorylation of ERK1/2 was increased in SAL group (P<0.05)

while AKT had no significant change. LY294002 further reduced IS

whereas the protective role of salvianolate could be attenuated by PD98059

which increased the IS. Additionally

the IS was not linearly related to the RR (r=0.23

0.45

0.62

0.17

and 0.52 in the SHAM

I/R

SAL

LY and PD groups

respectively). Salvianolate could reduce myocardial I/R injury in mice in vivo

which involves an ERK1/2 pathway

but not a PI3-K signaling pathway.

Abstract

I/R (no salvianolate)

and ischemia preconditioning (IPC) groups. Furthermore

an ERK1/2 inhibitor PD98059 (1 mg/kg)

and a phosphatidylinositol-3-kinase (PI3-K) inhibitor

LY294002 (7.5 mg/kg)

were administered intraperitoneal injection (i.p) for 30 min prior to salvianolate

followed by I/R surgery in LY and PD groups. By using a double staining method

I/R

IPC and SAL groups (P>0.05). The SAL and IPC groups had IS of 26.1%±1.4% and 22.3%±2.9% of RR

respectively

both of which were significantly smaller than the I/R group (38.5%±2.9% of RR

P<0.05

P<0.01

respectively). Moreover

the phosphorylation of ERK1/2 was increased in SAL group (P<0.05)

while AKT had no significant change. LY294002 further reduced IS

whereas the protective role of salvianolate could be attenuated by PD98059

which increased the IS. Additionally

the IS was not linearly related to the RR (r=0.23

0.45

0.62

0.17

and 0.52 in the SHAM

I/R

SAL

LY and PD groups

respectively). Salvianolate could reduce myocardial I/R injury in mice in vivo

which involves an ERK1/2 pathway

but not a PI3-K signaling pathway.

关键词

ischemia and reperfusion injurysalvianolateextracellular signal-regulated kinase 1/2protein kinase BChinese Medicine

Keywords

ischemia and reperfusion injurysalvianolateextracellular signal-regulated kinase 1/2protein kinase BChinese Medicine

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