Brucine inhibits bone metastasis of breast cancer cells by suppressing Jagged1/Notch1 signaling pathways

Ke-fei Hu; Xiang-ying Kong; Mi-cun Zhong; Hong-ye Wan; Na Lin; Xiao-hua Pei

doi:10.1007/s11655-016-2647-2

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Brucine inhibits bone metastasis of breast cancer cells by suppressing Jagged1/Notch1 signaling pathways

OriginalPaper | Updated：2021-08-27

- Brucine inhibits bone metastasis of breast cancer cells by suppressing Jagged1/Notch1 signaling pathways
- Brucine inhibits bone metastasis of breast cancer cells by suppressing Jagged1/Notch1 signaling pathways
- 中国结合医学杂志（英文版） 2017年23卷第2期页码：110-116
- Affiliations：
  
  1. Beijing University of Chinese Medicine,Beijing,China
  2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences,Beijing,China
  3. Department of Scientific Research and Education, Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing,China
- Author bio：
- Funds：
  
  Supported by the Fifty-fifth Batch of China Post Doctoral Science Foundation (No. 2014M550663)
- DOI：10.1007/s11655-016-2647-2
  中图分类号：
- 纸质出版日期：2017，
  
  网络出版日期：2016-12-29，
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Hu, Kf., Kong, Xy., Zhong, Mc. et al. Brucine inhibits bone metastasis of breast cancer cells by suppressing Jagged1/Notch1 signaling pathways., Chin. J. Integr. Med. 23, 110–116 (2017). https://doi.org/10.1007/s11655-016-2647-2

Ke-fei Hu, Xiang-ying Kong, Mi-cun Zhong, et al. Brucine inhibits bone metastasis of breast cancer cells by suppressing Jagged1/Notch1 signaling pathways[J]. Chinese Journal of Integrative Medicine, 2017,23(2):110-116.
Hu, Kf., Kong, Xy., Zhong, Mc. et al. Brucine inhibits bone metastasis of breast cancer cells by suppressing Jagged1/Notch1 signaling pathways., Chin. J. Integr. Med. 23, 110–116 (2017). https://doi.org/10.1007/s11655-016-2647-2 DOI：

Ke-fei Hu, Xiang-ying Kong, Mi-cun Zhong, et al. Brucine inhibits bone metastasis of breast cancer cells by suppressing Jagged1/Notch1 signaling pathways[J]. Chinese Journal of Integrative Medicine, 2017,23(2):110-116. DOI： 10.1007/s11655-016-2647-2.

摘要

To examine the effects of brucine on the invasion

migration and bone resorption of receptor activator of nuclear factor-kappa B ligand (RANKL)-induced osteoclastogenesis. The osteoclastogenesis model was builded by co-culturing human breast tumor MDA-MB-231 and mouse RAW264.7 macrophages cells. RANKL (50 ng/mL) and macrophage-colony stimulating factor (50 ng/mL) were added to this system

followed by treatment with brucine (0.02

0.04 and 0.08 mmol/L)

or 10 μmol/L zoledronic acid as positive control. The migration and bone resorption were measured by transwell assay and in vitro bone resorption assay. The protein expressions of Jagged1 and Notch1 were investigated by Western blot. The expressions of transforming growth factor-β1 (TGF-β1)

nuclear factor-kappa B (NF-κB) and Hes1 were determined by enzyme-linked immunosorbent assay. Compared with the model group

brucine led to a dose-dependent decrease on migration of MDA-MB-231 cells

inhibited RANKL-induced osteoclastogenesis and bone resorption of RAW264.7 cells (P<0.01). Furthermore

brucine decreased the protein levels of Jagged1 and Notch1 in MDA-MB-231 cells and RAW264.7 cells co-cultured system as well as the expressions of TGF-β1

NF-κB and Hes1 (P<0.05 or P<0.01). Brucine may inhibit osteoclastogenesis by suppressing Jagged1/Notch1 signaling pathways.

Abstract

To examine the effects of brucine on the invasion

followed by treatment with brucine (0.02

0.04 and 0.08 mmol/L)

nuclear factor-kappa B (NF-κB) and Hes1 were determined by enzyme-linked immunosorbent assay. Compared with the model group

brucine led to a dose-dependent decrease on migration of MDA-MB-231 cells

inhibited RANKL-induced osteoclastogenesis and bone resorption of RAW264.7 cells (P<0.01). Furthermore

brucine decreased the protein levels of Jagged1 and Notch1 in MDA-MB-231 cells and RAW264.7 cells co-cultured system as well as the expressions of TGF-β1

NF-κB and Hes1 (P<0.05 or P<0.01). Brucine may inhibit osteoclastogenesis by suppressing Jagged1/Notch1 signaling pathways.

关键词

BrucineBreast CancerBone MetastasisJagged1/Notch1 signaling pathway

Keywords

BrucineBreast CancerBone MetastasisJagged1/Notch1 signaling pathway

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