Protective effect of hydroxysafflor yellow A on bleomycin- induced pulmonary inflammation and fibrosis in rats

Ming Jin; Lin Wang; Yan Wu; Bao-xia Zang; Li Tan

doi:10.1007/s11655-017-2094-z

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Protective effect of hydroxysafflor yellow A on bleomycin- induced pulmonary inflammation and fibrosis in rats

OriginalPaper | Updated：2021-08-27

- Protective effect of hydroxysafflor yellow A on bleomycin- induced pulmonary inflammation and fibrosis in rats
- Protective effect of hydroxysafflor yellow A on bleomycin- induced pulmonary inflammation and fibrosis in rats
- 中国结合医学杂志（英文版） 2018年24卷第1期页码：32-39
- Affiliations：
  
  Department of Pharmacology, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing Anzhen Hospital, Capital Medical University,Beijing,China
- Author bio：
- Funds：
  
  Supported by Traditional Chinese Medicine Development Foundation of Beijing (No. JJ-2009-22), and Natural Science Foundation of Beijing (No. 7132047)
- DOI：10.1007/s11655-017-2094-z
  中图分类号：
- 纸质出版日期：2018，
  
  网络出版日期：2018-1-3，
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Jin, M., Wang, L., Wu, Y. et al. Protective effect of hydroxysafflor yellow A on bleomycin- induced pulmonary inflammation and fibrosis in rats., Chin. J. Integr. Med. 24, 32–39 (2018). https://doi.org/10.1007/s11655-017-2094-z

Ming Jin, Lin Wang, Yan Wu, et al. Protective effect of hydroxysafflor yellow A on bleomycin- induced pulmonary inflammation and fibrosis in rats[J]. Chinese Journal of Integrative Medicine, 2018,24(1):32-39.
Jin, M., Wang, L., Wu, Y. et al. Protective effect of hydroxysafflor yellow A on bleomycin- induced pulmonary inflammation and fibrosis in rats., Chin. J. Integr. Med. 24, 32–39 (2018). https://doi.org/10.1007/s11655-017-2094-z DOI：

Ming Jin, Lin Wang, Yan Wu, et al. Protective effect of hydroxysafflor yellow A on bleomycin- induced pulmonary inflammation and fibrosis in rats[J]. Chinese Journal of Integrative Medicine, 2018,24(1):32-39. DOI： 10.1007/s11655-017-2094-z.

摘要

To observe the effect of hydroxysafflor yellow A (HSYA)

an active ingredient of a traditional Chinese herbal medicine Carthamus tinctorius L.

on lung inflflammation and pulmonary fibrosis induced by bleomycin (BLM) in rats. Animals were divided into 6 groups including normal group

model group

three HSYA groups and dexamethasone (DXM) group. Three doses of HSYA (35.6

53.3

and 80.0 mg•kg–1•day–1) were intraperitoneally (i.p.) injected in rats for 3 weeks after BLM administration and DXM was used as the positive control (n=8 or 12). Arterial blood gas was assayed and morphological changes were observed. Lung mRNA expressions of tumor necrosis factor (TNF)-α

interleukin (IL)-1β

IL-6 and some cytokines in lung tissue were detected by real-time polymerase chain reaction. Nuclear factor-κB p65 or α-smooth muscle actin (α-SMA) protein distribution in rat lung tissue was observed by immunohistochemistry. On the 7th day after BLM administration

lung tissue showed serious inflammation. Treatment with HSYA or DXM ameliorated lung inflammation. After treatment with HSYA or DXM

oxygen partial pressure (PaO2) increased (HSYA 80.0 mg•kg–1

P<0.01) and CO2 partial pressure (PaCO2) decreased (HSYA 53.3

80.0 mg•kg–1

P<0.05). Moreover

the mRNA expression of TNF-α

IL-1β

and IL-6; and the number of NF-κB p65 positive cells was lower in HSYA 53.3 and 80.0 mg•kg–1 groups than those in the model group (all P<0.05). Twenty-one days after BLM administration

HSYA or DXM treatment ameliorated fibrosis

increased PaO2 (HSYA 53.3

80.0 mg•kg–1

P<0.01)

and decreased PaCO2 (53.3 and 80.0 mg•kg–1

P<0.05). Further

the mRNA expression of TGF-β1

α-SMA

and collagen I as well as the number of α-SMA positive cells increased in the model group and HSYA can attenuate these changes (53.3

80.0 mg•kg–1

P<0.05). Hematoxylin and eosin and Masson's trichrome staining indicated that the fibrosis and collagen deposition were ameliorated in HSYA groups (53.3

80.0 mg•kg–1

P<0.05). HSYA could alleviate acute lung inflflammation and chronic pulmonary fibrosis induced by BLM in rats.

Abstract

To observe the effect of hydroxysafflor yellow A (HSYA)

an active ingredient of a traditional Chinese herbal medicine Carthamus tinctorius L.

on lung inflflammation and pulmonary fibrosis induced by bleomycin (BLM) in rats. Animals were divided into 6 groups including normal group

model group

three HSYA groups and dexamethasone (DXM) group. Three doses of HSYA (35.6

53.3

interleukin (IL)-1β

lung tissue showed serious inflammation. Treatment with HSYA or DXM ameliorated lung inflammation. After treatment with HSYA or DXM

oxygen partial pressure (PaO2) increased (HSYA 80.0 mg•kg–1

P<0.01) and CO2 partial pressure (PaCO2) decreased (HSYA 53.3

80.0 mg•kg–1

P<0.05). Moreover

the mRNA expression of TNF-α

IL-1β

and IL-6; and the number of NF-κB p65 positive cells was lower in HSYA 53.3 and 80.0 mg•kg–1 groups than those in the model group (all P<0.05). Twenty-one days after BLM administration

HSYA or DXM treatment ameliorated fibrosis

increased PaO2 (HSYA 53.3

80.0 mg•kg–1

P<0.01)

and decreased PaCO2 (53.3 and 80.0 mg•kg–1

P<0.05). Further

the mRNA expression of TGF-β1

α-SMA

and collagen I as well as the number of α-SMA positive cells increased in the model group and HSYA can attenuate these changes (53.3

80.0 mg•kg–1

P<0.05). Hematoxylin and eosin and Masson's trichrome staining indicated that the fibrosis and collagen deposition were ameliorated in HSYA groups (53.3

80.0 mg•kg–1

P<0.05). HSYA could alleviate acute lung inflflammation and chronic pulmonary fibrosis induced by BLM in rats.

关键词

hydroxysafflor yellow APulmonary Fibrosispulmonary inflammationnuclear factor-κB p65α-smooth muscle actinChinese Medicine

Keywords

hydroxysafflor yellow APulmonary Fibrosispulmonary inflammationnuclear factor-κB p65α-smooth muscle actinChinese Medicine

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