FOLLOWUS
Synergistic effects of Chuanxiong-Chishao herb-pair on promoting angiogenesis at network pharmacological and pharmacodynamic levels
Synergistic effects of Chuanxiong-Chishao herb-pair on promoting angiogenesis at network pharmacological and pharmacodynamic levels
- 中国结合医学杂志（英文版） 2017年23卷第9期 页码：654-662
- Affiliations：
1. Clinical Medical College, Beijing University of Chinese Medicine,Beijing,China
2. Laboratory of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
3. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau,Macao SAR,China
4. Department of Acupuncture and Moxibustion, Qilu Hospital Affiliated to Shandong University,Jinan,China
- Author bio：
- Funds：Supported by Beijing Municipal Science and Technology Commission (No. Z141100002214011), the Science and Technology Development Fund of Macao SAR (No. 069/2015/A2 and 134/2014/A3) and Research Committee of University of Macau (No. MYRG2015-00182-ICMS-QRCM, MYRG2015-00214-ICMS-QRCM, MYRG139(Y1-L4)-ICMS12-LMY, and MYRG2016-00129-ICMS-QRCM), China;Conflict of Interests;The authors claimed no potential conflicts of interest relevant to this article.;Author Contributions;Wang Y and Guo G contributed equally to this work. Chen KJ, Lee SM, Cong WH, Yang BR, Xin QQ and Wang Y conceived and designed the experiments;Yang BR, HU YJ, Liao QW performed the network pharmacology analysis;Wang Y, Guo G, Xin QQ performed the zebrafish experiments. Wang Y and Guo G contributed significantly to analysis and manuscript preparation. Chen KJ, Lee SM and Cong WH helped perform the analysis with constructive discussions.;Electronic Supplementary Materials;: Supplementary materials (Appendixes 1-2) are available in the online version of this article at http://dx.doi.org/10.1007/s11655-017-2408-x
DOI：10.1007/s11655-017-2408-x
中图分类号：纸质出版日期：2017，
网络出版日期：2017-5-27，
Scan for full text
Wang, Y., Guo, G., Yang, Br. et al. Synergistic effects of Chuanxiong-Chishao herb-pair on promoting angiogenesis at network pharmacological and pharmacodynamic levels., Chin. J. Integr. Med. 23, 654–662 (2017). https://doi.org/10.1007/s11655-017-2408-x
Yan Wang, Gang Guo, Bin-rui Yang, et al. Synergistic effects of Chuanxiong-Chishao herb-pair on promoting angiogenesis at network pharmacological and pharmacodynamic levels[J]. Chinese Journal of Integrative Medicine, 2017,23(9):654-662.
Wang, Y., Guo, G., Yang, Br. et al. Synergistic effects of Chuanxiong-Chishao herb-pair on promoting angiogenesis at network pharmacological and pharmacodynamic levels., Chin. J. Integr. Med. 23, 654–662 (2017). https://doi.org/10.1007/s11655-017-2408-x DOI：
Yan Wang, Gang Guo, Bin-rui Yang, et al. Synergistic effects of Chuanxiong-Chishao herb-pair on promoting angiogenesis at network pharmacological and pharmacodynamic levels[J]. Chinese Journal of Integrative Medicine, 2017,23(9):654-662. DOI： 10.1007/s11655-017-2408-x.
摘要
To investigate the synergistic effects of Chuanxiong-Chishao herb-pair (CCHP) on promoting angiogenesis in silico and in vivo. The mechanisms of action of an herb-pair
Chuanxiong-Chishao
were investigated using the network pharmacological and pharmacodynamic strategies involving computational drug target prediction and network analysis
and experimental validation. A set of network pharmacology methods were created to study the herbs in the context of targets and diseases networks
including prediction of target profiles and pharmacological actions of main active compounds in Chuanxiong and Chishao. Furthermore
the therapeutic effects and putative molecular mechanisms of Chuanxiong-Chishao actions were experimentally validated in a chemical-induced vascular insuffificiency model of transgenic zebrafifish in vivo. The mRNA expression of the predicted targets were further analyzed by real-time polymerase chain reaction (RT-PCR). The computational prediction results found that the compounds in Chuanxiong have antithrombotic
antihypertensive
antiarrhythmic
and antiatherosclerotic activities
which were closely related to protecting against hypoxic-ischemic encephalopathy
ischemic stroke
myocardial infarction and heart failure. In addition
compounds in Chishao were found to participate in anti-inflflammatory effect and analgesics. Particularly
estrogen receptor α (ESRα) and hypoxia-inducible factor 1-α (HIF-1α) were the most important potential protein targets in the predicted results. In vivo experimental validation showed that post-treatment of tetramethylpyrazine hydrochloride (TMP•HCl) and paeoniflorin (PF) promoted the regeneration of new blood vessels in zebrafifish involving up-regulating ESRα mRNA expression. Co-treatment of TMP•HCl and PF could enhance the vessel sprouting in chemical-induced vascular insuffificiency zebrafifish at the optimal compatibility proportion of PF 10 μmol/L with TMP•HCl 1 μmol/L. The network pharmacological strategies combining drug target prediction and network analysis identified some putative targets of CCHP. Moreover
the transgenic zebrafifish experiments demonstrated that the Chuanxiong-Chishao combination synergistically promoted angiogenic activity
probably involving ESRα signaling pathway.
Abstract
To investigate the synergistic effects of Chuanxiong-Chishao herb-pair (CCHP) on promoting angiogenesis in silico and in vivo. The mechanisms of action of an herb-pair
Chuanxiong-Chishao
were investigated using the network pharmacological and pharmacodynamic strategies involving computational drug target prediction and network analysis
and experimental validation. A set of network pharmacology methods were created to study the herbs in the context of targets and diseases networks
including prediction of target profiles and pharmacological actions of main active compounds in Chuanxiong and Chishao. Furthermore
the therapeutic effects and putative molecular mechanisms of Chuanxiong-Chishao actions were experimentally validated in a chemical-induced vascular insuffificiency model of transgenic zebrafifish in vivo. The mRNA expression of the predicted targets were further analyzed by real-time polymerase chain reaction (RT-PCR). The computational prediction results found that the compounds in Chuanxiong have antithrombotic
antihypertensive
antiarrhythmic
and antiatherosclerotic activities
which were closely related to protecting against hypoxic-ischemic encephalopathy
ischemic stroke
myocardial infarction and heart failure. In addition
compounds in Chishao were found to participate in anti-inflflammatory effect and analgesics. Particularly
estrogen receptor α (ESRα) and hypoxia-inducible factor 1-α (HIF-1α) were the most important potential protein targets in the predicted results. In vivo experimental validation showed that post-treatment of tetramethylpyrazine hydrochloride (TMP•HCl) and paeoniflorin (PF) promoted the regeneration of new blood vessels in zebrafifish involving up-regulating ESRα mRNA expression. Co-treatment of TMP•HCl and PF could enhance the vessel sprouting in chemical-induced vascular insuffificiency zebrafifish at the optimal compatibility proportion of PF 10 μmol/L with TMP•HCl 1 μmol/L. The network pharmacological strategies combining drug target prediction and network analysis identified some putative targets of CCHP. Moreover
the transgenic zebrafifish experiments demonstrated that the Chuanxiong-Chishao combination synergistically promoted angiogenic activity
probably involving ESRα signaling pathway.
关键词
tetramethylpyrazinepaeoniflorinangiogenesisnetwork pharmacology
Keywords
tetramethylpyrazinepaeoniflorinangiogenesisnetwork pharmacology
references
Cheung F. TCM: made in China. Nature 2011;480:S82–S83.
Li SP, Zhao J, Yang B. Strategies for quality control of Chinese medicines. J Pharm Biomed Anal 2011;55:802–809.
Su SB, Jia W, Lu AP, Li S. Evidence-based Zheng: a traditional Chinese medicine syndrome 2013. Evid Based Complement Alternat Med 2014;2014:1–2.
Li P, Qi LW, Liu EH, Zhoun JL, Wen XD. Analysis of Chinese herbal medicines with holistic approaches and integrated evaluation models. Trends Analyt Chem 2008;27:66–77.
Qiu J. Traditional medicine: a culture in the balance. Nature 2007;448:126–128.
Tang F, Zhang QL, Nie Z, Yao SZ, Chen B. Sample preparation for analyzing traditional Chinese medicines. TrAC Trends Analytic Chem 2009;28:1253–1262.
The State Pharmacopoeia Commission of the People's Republic of China. Chinese Pharmacopoeia. Beijing: Chemical Industry Press; 2010;1:38.
Guo M, Liu Y, Shi DZ. Cardiovascular actions and therapeutic potential of tetramethylpyrazine (active component isolated from Rhizoma Chuanxiong): roles and mechanisms. Biomed Res Int 2016;2016:1–9.
Nizamutdinova IT, Jin YC, Kim JS, Yean MH, Kang SS, Kim YS, et al. Paeonol and paeoniflorin, the main active principles of Paeonia albiflora, protect the heart from myocardial ischemia/reperfusion injury in rats. Planta Med 2008;74:14–18.
Xin QQ, Yang BR, Zhou HF, Wang Y, Yi BW, Cong WH, et al. Paeoniflorin promotes angiogenesis in a vascular insufficiency model of zebrafish in vivo and in human umbilical vein endothelial cells in vitro. Chin J Integr Med 2016;1–8.
Franco CA, Liebner S, Gerhardt H. Vascular morphogenesis: a Wnt for every vessel? Curr Opin Genet Dev 2009;19:476–483.
Ahn A, Frishman WH, Gutwein A, Passeri J, Nelson M. Therapeutic angiogenesis: a new treatment approach for ischemic heart disease—part I. Cardiol Rev 2008;16:163–171.
He XH, Xing DM, Ding Y, Li Y, Xiang L, Wang W, et al. Determination of paeoniflorin in rat hippocampus by high performance liquid chromatography after intravenous administration of Paeoniae Radix extract. J Chromatogra B 2004;802:277–281.
Ru JL, Li P, Wang JN, Zhou W, Li BH, Huang C, et al. TCMSP: a database of systems pharmacology for drug discovery from herbal medicines. J Cheminformatics 2014;6:13.
Knox C, Law CKV, Jewison T, Liu P, Ly S, Frolkis A, et al. DrugBank 3.0: a comprehensive resource for 'omics' research on drugs. Nucleic Acids Res 2011;39 suppl 1:1035–1041.
Ye H, Ye L, Kang H, Zhang DF, Tao L, Tang KL, et al. HIT: linking herbal active ingredients to targets. Nucleic Acids Res 2011;39 suppl 1:1055–1059.
Yu H, Chen JX, Xu X, Li Y, Zhao HH, Fang YP, et al. A systematic prediction of multiple drug-target interactions from chemical, genomic, and pharmacological data. PLoS One 2012;7:e37608.
Zhu F, Shi Z, Qin C, Tao L, Liu X, Xu F, et al. Therapeutic target database update 2012: a resource for facilitating target-oriented drug discovery. Nucleic Acids Res 2011;40:1128–1136.
Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, Ramage D, et al. Cytoscape: a software environment for integrated models of biomolecular interaction networks. Genome Res 2003;13:2498–2504.
Azuaje FJ, Zhang L, Devaux Y, Wagner DR. Drug-target network in myocardial infarction reveals multiple side effects of unrelated drugs. Sci Reports 2011;1:52.
Li XX, Xu X, Wang JN, Yu H, Wang X, Yang HJ, et al. A system-level investigation into the mechanisms of Chinese traditional medicine: Compound Danshen Formula for cardiovascular disease treatment. PLoS One 2012;7:e43918.
Westerfield M, ed. A guide for the laboratory use of zebrafish (Danio rerio). The zebrafish book. 5th ed. Eugene: University of Oregon Press; 2007.
Kabir ME, Singh H, Lu R, Olde B, Leeb-Lundberg LM, Bopassa JC, et al. G protein-coupled estrogen receptor 1 mediates acute estrogen-induced cardioprotection via MEK/ ERK/GSK-3β pathway after ischemia/reperfusion. PLoS One 2015;10:e0135988.
Vecoli C. Endothelial nitric oxide synthase gene polymorphisms in cardiovascular disease. Vitam Horm 2014;96:387–406.
Frishman WH. Beta-adrenergic receptor blockers in hypertension: alive and well. Prog Cardiovasc Dis 2016;59:247–252.
Mohamed RA, Agha AM, Abdel-Rahman AA, Nassar NN. Role of adenosine A2A receptor in cerebral ischemia reperfusion injury: Signaling to phosphorylated extracellular signal-regulated protein kinase (pERK1/2). Neuroscience 2016;314:145–159.
Isogai S, Lawson ND, Torrealday S, Horiguchi M, Weinstein BM. Angiogenic network formation in the developing vertebrate trunk. Development 2003:130:5281–5290.
Furet P, Bold G, Hofmann F, Manley P, Meyer T, Altmann KH. Identification of a new chemical class of potent angiogenesis inhibitors based on conformational considerations and database searching. Bioorg Med Chem Lett 2003;13:2967–2971.
Rix U, Superti-Furga G. Target profiling of small molecules by chemical proteomics. Nature Chem Biol 2009;5:616–624.
Li XM, Brown L. Efficacy and mechanisms of action of traditional Chinese medicines for treating asthma and allergy. J Allergy Clin Immunol 2009;123:297–306.
Schmidt BM, Ribnicky DM, Lipsky PE, Raskin I. Revisiting the ancient concept of botanical therapeutics. Nature Chem Biol 2007;3:360–366.
Wang GL, Jiang BH, Rue EA, Semenza GL. Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension. Proc Natl Acad Sci 1995;92:5510–5514.
Iyer NV, Kotch LE, Agani F, Leung SW, Laughner E, Wenger RH, et al. Cellular and developmental control of O2 homeostasis by hypoxia-inducible factor 1α. Genes Dev 1998;12:149–162.
Nakada YJ, Canseco DC, Thet SW, Abdisalaam S, Asaithamby A, Santos CX, et al. Hypoxia induces heart regeneration in adult mice. Nature 2017;541:222–227.
Martínez-Lara E, Peña A, Calahorra J, Cañuelo A, Siles E. Hydroxytyrosol decreases the oxidative and nitrosative stress levels and promotes angiogenesis through HIF-1 independent mechanisms in renal hypoxic cells. Food Function 2016;7:540–548.
Arnal JF, Fontaine C, Billon-Galés A, Favre J, Laurell H, Lenfant F, et al. Estrogen receptors and endothelium. Arterioscl Thromb Vascul Biol 2010;30:1506–1512.
Gopal S, Garibaldi S, Goglia L, Polak K, Palla G, Spina S, et al. Estrogen regulates endothelial migration via plasminogen activator inhibitor (PAI-1). Mol Human Reprod 2012;18:410–416.
Caliceti C, Aquila G, Pannella M, Morelli MB, Fortini C, Pinton P, et al. 17β-Estradiol enhances signalling mediated by VEGF-A-delta-like ligand 4-notch1 axis in human endothelial cells. PloS One 2013:8:e71440.
Li S, Dang YY, Zhou XL, Huang B, Huang XH, Zhang ZR, et al. Formononetin promotes angiogenesis through the estrogen receptor alpha-enhanced ROCK pathway. Sci Reports 2015;5:16815.
Cai XX, Chen Z, Pan XK, Xia L, Chen P, Yang Y, et al. Inhibition of angiogenesis, fibrosis and thrombosis by tetramethylpyrazine: mechanisms contributing to the SDF-1/CXCR4 axis. PLoS One 2014;9:e88176.
Jia YC, Wang ZG, Zang AM, Jiao SC, Chen SM, Fu Y. Tetramethylpyrazine inhibits tumor growth of lung cancer through disrupting angiogenesis via BMP/Smad/Id-1 signaling. Int J Oncol 2016;48:2079–2086.
0
浏览量
0
Downloads
9
CSCD
- Meta-XML下载
- BibTeX下载
- Endnote下载
- RefMan 下载
- NoteExpress下载
- NoteFirst下载
关联资源
相关文章
相关作者
相关机构
- 邮编：100091
- 联系电话：010-62886827, 62877592 传真：010-62874291
- 技术支持由北京北大方正电子有限公司提供 京ICP备05067934号-1
京公网安备11010802024621 - 本系统建议在Chrome、 IE9+ 以上版本浏览器阅读本站内容，360浏览器请切换至极速模式
- Cookies帮助我们提供服务并提供个性化体验。使用本网站，即表示您同意我们使用Cookies
